Development of a quantitative bioassay to assess preventive compounds against inflammation-based carcinogenesis

Nitric Oxide ◽  
2011 ◽  
Vol 25 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Kunishige Onuma ◽  
Yuka Suenaga ◽  
Ryuta Sakaki ◽  
Satoshi Yoshitome ◽  
Yu Sato ◽  
...  
Keyword(s):  
Quantitative Bioassay

BIOASSAY CHARACTERISTICS OF INDIVIDUAL URINARY GONADOTROPHIN EXTRACTS

1963 ◽  
Vol 42 (2) ◽  
pp. 214-224
Author(s):  
Raymond C. Mellinger ◽  
Jalileh A. Mansour ◽  
Richmond W. Smith
Keyword(s):  
Reference Material ◽  
Young Women ◽  
Reference Standard ◽  
General Acceptance ◽  
Modifying Factors ◽  
Large Numbers ◽  
The World ◽  
Quantitative Bioassay ◽  
Different Parts ◽  
Age And Sex

ABSTRACT A reference standard is widely sought for use in the quantitative bioassay of pituitary gonadotrophin recovered from urine. The biologic similarity of pooled urinary extracts obtained from large numbers of subjects, utilizing groups of different age and sex, preparing and assaying the materials by varying techniques in different parts of the world, has lead to a general acceptance of such preparations as international gonadotrophin reference standards. In the present study, however, the extract of pooled urine from a small number of young women is shown to produce a significantly different bioassay response from that of the reference materials. Gonadotrophins of individual subjects likewise varied from the multiple subject standards in many instances. The cause of these differences is thought to be due to the modifying influence of non-hormonal substances extracted from urine with the gonadotrophin and not necessarily to variations in the gonadotrophins themselves. Such modifying factors might have similar effects in a comparative assay of pooled extracts contributed by many subjects, but produce significant variations when material from individual subjects is compared. It is concluded that the expression of potency of a gonadotrophic extract in terms of pooled reference material to which it is not essentially similar may diminish rather than enhance the validity of the assay.

A Microbiological Assay for Penicillic Acid1

1978 ◽  
Vol 41 (6) ◽  
pp. 432-434 ◽  
Author(s):  
F. J. OLIVIGNI ◽  
L. B. BULLERMAN
Keyword(s):  
Bacillus Subtilis ◽  
Biological Activity ◽  
Thin Layer ◽  
Linear Relationship ◽  
Acid Concentration ◽  
Spore Suspension ◽  
Liquid Media ◽  
Penicillic Acid ◽  
Bioassay Method ◽  
Quantitative Bioassay

Six bactertial cultures were studied in a search for an organism sensitive to penicillic acid suitable for use in a quantitative bioassay of this mycotoxin. A vegetative culture and a commercially prepared spore suspension of Bacillus subtilis were both sensitive to as little as 1 μg of penicillic acid and exhibited a linear relationship between 1 and 100 μg. The bioassay method was comparable in accuracy to thin layer chromatographic assay. The procedure was used to verify the biological activity of sample extracts, as well as to quantitate penicillic acid concentration in samples of liquid media and corn. The bioassay is sensitive, rapid (15–17 h), simple and inexpensive.

scholarly journals Surface-Enhanced Raman Spectroscopy Based Quantitative Bioassay on Aptamer-Functionalized Nanopillars Using Large-Area Raman Mapping

ACS Nano ◽  
2013 ◽  
Vol 7 (6) ◽  
pp. 5350-5359 ◽  
Author(s):  
Jaeyoung Yang ◽  
Mirko Palla ◽  
Filippo Giacomo Bosco ◽  
Tomas Rindzevicius ◽  
Tommy Sonne Alstrøm ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Mapping ◽  
Large Area ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Quantitative Bioassay

A quantitative bioassay for HIV-1 based on trans-activation

Science ◽  
1988 ◽  
Vol 239 (4836) ◽  
pp. 184-187 ◽  
Author(s):  
B. Felber ◽  
G. Pavlakis
Keyword(s):  
Quantitative Bioassay ◽  
Hiv 1

A novel quantitative bioassay for newly synthesized compounds as HIV-1 Tat–TAR RNA interaction inhibitors

2008 ◽  
Vol 136 ◽  
pp. S75
Author(s):  
Ming Yang ◽  
Meizi He ◽  
Ruifang Pang ◽  
Chunlei Zhang ◽  
Fei Yu ◽  
...  
Keyword(s):  
Tar Rna ◽  
Rna Interaction ◽  
Quantitative Bioassay ◽  
Hiv 1
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