Nitric oxide mediated oxidative stress injury in rat skeletal muscle subjected to ischemia/reperfusion as evaluated by chemiluminescence

Nitric Oxide ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 196-203 ◽  
Author(s):  
Karina Zimiani ◽  
Flávia Alessandra Guarnier ◽  
Helen Cristrina Miranda ◽  
Maria Angelica Ehara Watanabe ◽  
Rubens Cecchini
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Ischemia Reperfusion ◽  
Rat Skeletal Muscle ◽  
Stress Injury ◽  
Oxidative Stress Injury

scholarly journals Antifatigue Effect of Luteolin-6-C-Neohesperidoside on Oxidative Stress Injury Induced by Forced Swimming of Rats through Modulation of Nrf2/ARE Signaling Pathways

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Fang-fang Duan ◽  
Ying Guo ◽  
Jing-wan Li ◽  
Ke Yuan
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Inflammatory Responses ◽  
Interleukin 1 ◽  
Chronic Fatigue ◽  
Forced Swimming ◽  
Sprague Dawley ◽  
Stress Injury ◽  
Metabolic Markers ◽  
Oxidative Stress Injury

Luteolin-6-C-neohesperidoside (LN) is a flavonoid isolated from moso bamboo leaf. This study was performed to evaluate the antifatigue effect of LN on a rat model undergoing the weight-loaded forced swimming test (FST). Briefly, male Sprague-Dawley rats (20–22 weeks old) were forced to undertake exhaustive swimming every other day for 3 weeks. Each swimming session was followed by the administration of distilled water, LN (25–75 mg/kg), or ascorbic acid (100 mg/kg) 1 h later. Oral administration of LN significantly improved exercise endurance; normalized alterations in energy metabolic markers; and decreased serum lactic acid, lactate dehydrogenase, and blood urea nitrogen levels of rats that underwent FST. Moreover, LN enhanced the activities of antioxidant enzymes and antioxidant capacity, as measured by enzyme activity assays, RT-PCR, and Western blotting, as well as decreasing the levels of proinflammatory cytokines such as tumor necrosis factor-α, interleukin-1β(IL-1β), and IL-6 and increasing the level of anti-inflammatory (IL-10) in the liver and skeletal muscle. These results suggested that LN reduces both physical and mental effects of chronic fatigue, probably by attenuating oxidative stress injury and inflammatory responses in the liver and skeletal muscle. This study thus supports the use of LN in functional foods for antifatigue and antioxidant effects.

scholarly journals Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Jianfeng Liu ◽  
Ping Zhu ◽  
Peng Song ◽  
Weiping Xiong ◽  
Haixu Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Therapeutic Potential ◽  
Heart Function ◽  
Ischemia Reperfusion ◽  
Ischemic Myocardium ◽  
Myocardial Recovery ◽  
Myocardial Repair ◽  
Stress Injury ◽  
Oxidative Stress Injury

The poor survival rate of transplanted stem cells in ischemic myocardium has limited their therapeutic efficacy. Curcumin has potent antioxidant property. This study investigates whether prior curcumin treatment protects stem cells from oxidative stress injury and improves myocardial recovery following cells transplantation. Autologous Sprague-Dawley rat adipose derived mesenchymal stem cells (ADSCs) were pretreated with or without curcumin. The hydrogen peroxide/serum deprivation (H2O2/SD) medium was used to mimic the ischemic conditionin vitro. Cytoprotective effects of curcumin on ADSCs were evaluated. Curcumin pretreatment significantly increased cell viability and VEGF secretion, and decreased cell injury and apoptosis via regulation of PTEN/Akt/p53 and HO-1 signal proteins expression. The therapeutic potential of ADSCs implantation was investigated in myocardial ischemia-reperfusion injury (IRI) model. Transplantation of curcumin pretreated ADSCs not only resulted in better heart function, higher cells retention, and smaller infarct size, but also decreased myocardial apoptosis, promoted neovascularization, and increased VEGF level in ischemic myocardium. Together, priming of ADSCs with curcumin improved tolerance to oxidative stress injury and resulted in enhancement of their therapeutic potential of ADSCs for myocardial repair. Curcumin pretreatment is a promising adjuvant strategy for stem cells transplantation in myocardial restoration.

scholarly journals Bone marrow mesenchymal stem cells stimulated by tumor necrosis factor-α can promote the repair of fatty liver cell oxidative stress injury and fatty liver ischemia-reperfusion injury

2021 ◽  
Author(s):  
Yuying Tan ◽  
Jiali Qiu ◽  
Weiqi Zhang ◽  
Yan Xie ◽  
Chiyi Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fatty Liver ◽  
Liver Cell ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Stress Injury ◽  
Oxidative Stress Injury ◽  
Tnf Α

Mesenchymal stem cells (MSCs) have great prospects for the treatment of ischemia-reperfusion injury (IRI) after liver transplantation. At this stage, the main factor limiting MSCs in the treatment of fatty liver IRI of the donor liver is the residence time of stem cells at the site of inflammatory injury. This study investigated whether bone marrow mesenchymal stem cells (BMSCs) stimulated by tumor necrosis factor-α (TNF-α) can promote the repair of fatty liver cell oxidative stress injury and fatty liver IRI in rats. The results indicated the BMSCs treatment group stimulated by TNF-α had lower indexes and significantly improved oxidative stress damage in vitro through Transwell chamber co-culture experiment, compared with the control group. In vivo, compared with the PBS group and the BMSCs group, the indexes of the BMSCs treatment group stimulated by TNF-α were reduced, and the degree of tissue damage was significantly reduced. BMSCs can repair fatty liver cell oxidative stress injury and fatty liver IRI, however, BMSCs stimulated by TNF-α can promote the repair of tissues and cells.

scholarly journals H2O2 and Nitric Oxide-mediated Oxidative Stress Induce Apoptosis in Rat Skeletal Muscle Myoblasts

1996 ◽  
Vol 55 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Martin Stangel ◽  
Uwe K. Zettl ◽  
Eilhard Mix ◽  
Jürgen Zielasek ◽  
Klaus V. Toyka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Rat Skeletal Muscle

Nitric Oxide Synthase-Independent Generation of Nitric Oxide in Rat Skeletal Muscle Ischemia-Reperfusion Injury

Nitric Oxide ◽  
1999 ◽  
Vol 3 (1) ◽  
pp. 75-84 ◽  
Author(s):  
D.A. Lepore ◽  
A.V. Kozlov ◽  
A.G. Stewart ◽  
J.V. Hurley ◽  
W.A. Morrison ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Nitric Oxide Synthase ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Rat Skeletal Muscle ◽  
Muscle Ischemia ◽  
Oxide Synthase
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