Phase contrast enhanced high resolution X-ray imaging and tomography of soft tissue

2007 ◽  
Vol 571 (1-2) ◽  
pp. 69-72 ◽  
Author(s):  
Jan Jakubek ◽  
Carlos Granja ◽  
Jiri Dammer ◽  
Robert Hanus ◽  
Tomas Holy ◽  
...  
Keyword(s):  
High Resolution ◽  
Soft Tissue ◽  
Phase Contrast ◽  
X Ray ◽  
X Ray Imaging ◽  
Contrast Enhanced

scholarly journals Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue Tumors

2021 ◽  
Vol 20 ◽  
pp. 153303382110101
Author(s):  
Thet-Thet Lwin ◽  
Akio Yoneyama ◽  
Hiroko Maruyama ◽  
Tohoru Takeda
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
Soft Tissue Tumors ◽  
X Ray ◽  
3 Dimensional ◽  
Computed Tomography Images ◽  
X Ray Imaging ◽  
X Ray Computed

Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.

scholarly journals Early Tumor Development Captured Through Nondestructive, High Resolution Differential Phase Contrast X-ray Imaging

2013 ◽  
Vol 180 (5) ◽  
pp. 448-454 ◽  
Author(s):  
A. Beheshti ◽  
B. R. Pinzer ◽  
J. T. McDonald ◽  
M. Stampanoni ◽  
L. Hlatky
Keyword(s):  
High Resolution ◽  
Phase Contrast ◽  
Tumor Development ◽  
X Ray ◽  
Differential Phase ◽  
X Ray Imaging ◽  
Differential Phase Contrast ◽  
Early Tumor

Enhanced renal image contrast by ethanol fixation in phase-contrast X-ray computed tomography

2014 ◽  
Vol 21 (4) ◽  
pp. 795-800 ◽  
Author(s):  
Ryota Shirai ◽  
Takuya Kunii ◽  
Akio Yoneyama ◽  
Takahito Ooizumi ◽  
Hiroko Maruyama ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Phase Contrast ◽  
Image Contrast ◽  
Fixation Technique ◽  
Outer Medulla ◽  
X Ray ◽  
X Ray Imaging ◽  
Fixation Techniques ◽  
Outer Stripe ◽  
Fine Structures

Phase-contrast X-ray imaging using a crystal X-ray interferometer can depict the fine structures of biological objects without the use of a contrast agent. To obtain higher image contrast, fixation techniques have been examined with 100% ethanol and the commonly used 10% formalin, since ethanol causes increased density differences against background due to its physical properties and greater dehydration of soft tissue. Histological comparison was also performed. A phase-contrast X-ray system was used, fitted with a two-crystal X-ray interferometer at 35 keV X-ray energy. Fine structures, including cortex, tubules in the medulla, and the vessels of ethanol-fixed kidney could be visualized more clearly than that of formalin-fixed tissues. In the optical microscopic images, shrinkage of soft tissue and decreased luminal space were observed in ethanol-fixed kidney; and this change was significantly shown in the cortex and outer stripe of the outer medulla. The ethanol fixation technique enhances image contrast by approximately 2.7–3.2 times in the cortex and the outer stripe of the outer medulla; the effect of shrinkage and the physical effect of ethanol cause an increment of approximately 78% and 22%, respectively. Thus, the ethanol-fixation technique enables the image contrast to be enhanced in phase-contrast X-ray imaging.

Characterization using Phase-Contrast Enhanced X-Ray Imaging

2005 ◽  
Author(s):  
Bernard Kozioziemski ◽  
John D. Moody ◽  
James D. Sater ◽  
Harry E. Martz ◽  
Anton Barty ◽  
...  
Keyword(s):  
Phase Contrast ◽  
X Ray ◽  
X Ray Imaging ◽  
Contrast Enhanced

scholarly journals High Resolution Phase Contrast X-ray Imaging of Cosmic Dust Captured in Aerogel

2005 ◽  
Vol 11 (S02) ◽  
Author(s):  
J Sheffield-Parker ◽  
G A Graham ◽  
S C Mayo ◽  
N Teslich ◽  
P G Grant ◽  
...  
Keyword(s):  
High Resolution ◽  
Phase Contrast ◽  
Cosmic Dust ◽  
X Ray ◽  
X Ray Imaging

scholarly journals High-throughput, high-resolution X-ray phase contrast tomographic microscopy for visualisation of soft tissue

2009 ◽  
Vol 186 ◽  
pp. 012043 ◽  
Author(s):  
S A McDonald ◽  
F Marone ◽  
C Hintermüller ◽  
J-C Bensadoun ◽  
P Aebischer ◽  
...  
Keyword(s):  
High Resolution ◽  
Soft Tissue ◽  
High Throughput ◽  
Phase Contrast ◽  
X Ray

scholarly journals A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria Pierantoni ◽  
Isabella Silva Barreto ◽  
Malin Hammerman ◽  
Lissa Verhoeven ◽  
Elin Törnquist ◽  
...  
Keyword(s):  
High Resolution ◽  
Soft Tissue ◽  
Image Quality ◽  
Achilles Tendon ◽  
Phase Contrast ◽  
Optimization Approach ◽  
Mechanical Stimuli ◽  
Invasive Approach ◽  
Contrast Enhanced ◽  
Micro Tomography

AbstractAchilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.

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