scholarly journals Cell Types, Network Homeostasis, and Pathological Compensation from a Biologically Plausible Ion Channel Expression Model

Neuron ◽  
2014 ◽  
Vol 82 (4) ◽  
pp. 809-821 ◽  
Author(s):  
Timothy O’Leary ◽  
Alex H. Williams ◽  
Alessio Franci ◽  
Eve Marder
Keyword(s):  
Ion Channel ◽  
Cell Types ◽  
Channel Expression ◽  
Expression Model

scholarly journals Cell Types, Network Homeostasis, and Pathological Compensation from a Biologically Plausible Ion Channel Expression Model

Neuron ◽  
2015 ◽  
Vol 88 (6) ◽  
pp. 1308 ◽  
Author(s):  
Timothy O’Leary ◽  
Alex H. Williams ◽  
Alessio Franci ◽  
Eve Marder
Keyword(s):  
Ion Channel ◽  
Cell Types ◽  
Channel Expression ◽  
Expression Model

VOCCs and TREK-1 ion channel expression in human tenocytes

2007 ◽  
Vol 292 (3) ◽  
pp. C1053-C1060 ◽  
Author(s):  
Merzesh Magra ◽  
Steven Hughes ◽  
Alicia J. El Haj ◽  
Nicola Maffulli
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Ionic Currents ◽  
Cell Types ◽  
Functional Expression ◽  
Tissue Cell ◽  
Pharmacological Management ◽  
Whole Cell ◽  
Voltage Gated ◽  
Channel Expression

Mechanosensitive and voltage-gated ion channels are known to perform important roles in mechanotransduction in a number of connective tissues, including bone and muscle. It is hypothesized that voltage-gated and mechanosensitive ion channels also may play a key role in some or all initial responses of human tenocytes to mechanical stimulation. However, to date there has been no direct investigation of ion channel expression by human tenocytes. Human tenocytes were cultured from patellar tendon samples harvested from five patients undergoing routine total knee replacement surgery (mean age: 66 yr; range: 63–73 yr). RT-PCR, Western blotting, and whole cell electrophysiological studies were performed to investigate the expression of different classes of ion channels within tenocytes. Human tenocytes expressed mRNA and protein encoding voltage-operated calcium channel (VOCC) subunits (Ca α1A, Ca α1C, Ca α1D, Ca α2δ1) and the mechanosensitive tandem pore domain potassium channel (2PK+) TREK-1. They exhibit whole cell currents consistent with the functional expression of these channels. In addition, other ionic currents were detected within tenocytes consistent with the expression of a diverse array of other ion channels. VOCCs and TREK channels have been implicated in mechanotransduction signaling pathways in numerous connective tissue cell types. These mechanisms may be present in human tenocytes. In addition, human tenocytes may express other channel currents. Ion channels may represent potential targets for the pharmacological management of chronic tendinopathies.

Differential expression of ORCC and CFTR induced by low temperature in CF airway epithelial cells

1995 ◽  
Vol 268 (1) ◽  
pp. C243-C251 ◽  
Author(s):  
M. E. Egan ◽  
E. M. Schwiebert ◽  
W. B. Guggino
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Incubation Temperature ◽  
Cell Types ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Channel Activity ◽  
Patch Clamp Recording ◽  
Cl Channel ◽  
Channel Expression

When nonepithelial cell types expressing the delta F508-cystic fibrosis transmembrane conductance regulator (CFTR) mutation are grown at reduced temperatures, the mutant protein can be properly processed. The effect of low temperatures on Cl- channel activity in airway epithelial cells that endogenously express the delta F508-CFTR mutation has not been investigated. Therefore, we examined the effect of incubation temperature on both CFTR and outwardly rectifying Cl- channel (ORCC) activity in normal, in cystic fibrosis (CF)-affected, and in wild-type CFTR-complemented CF airway epithelia with use of a combination of inside-out and whole cell patch-clamp recording, 36Cl- efflux assays, and immunocytochemistry. We report that incubation of CF-affected airway epithelial cells at 25-27 degrees C is associated with the appearance of a protein kinase A-stimulated CFTR-like Cl- conductance. In addition to the appearance of CFTR Cl- channel activity, there is, however, a decrease in the number of active ORCC when cells are grown at 25-27 degrees C, suggesting that the decrease in incubation temperature may be associated with multiple alterations in ion channel expression and/or regulation in airway epithelial cells.

Changes in ion channel expression and function associated with cardiac arrhythmogenic remodeling by Sorbs2

2021 ◽  
Vol 1867 (12) ◽  
pp. 166247
Author(s):  
Ling-Ling Qian ◽  
Xiaojing Sun ◽  
Jingchun Yang ◽  
Xiao-Li Wang ◽  
Michael J. Ackerman ◽  
...  
Keyword(s):  
Ion Channel ◽  
Channel Expression ◽  
And Function

scholarly journals Ion channel expression patterns in glioblastoma stem cells with functional and therapeutic implications for malignancy

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0172884 ◽  
Author(s):  
Julia Pollak ◽  
Karan G. Rai ◽  
Cory C. Funk ◽  
Sonali Arora ◽  
Eunjee Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ion Channel ◽  
Expression Patterns ◽  
Glioblastoma Stem Cells ◽  
Therapeutic Implications ◽  
Channel Expression

scholarly journals Kv2.1 cell surface clusters are insertion platforms for ion channel delivery to the plasma membrane

2012 ◽  
Vol 23 (15) ◽  
pp. 2917-2929 ◽  
Author(s):  
Emily Deutsch ◽  
Aubrey V. Weigel ◽  
Elizabeth J. Akin ◽  
Phil Fox ◽  
Gentry Hansen ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Cell Surface ◽  
Ion Channel ◽  
Protein Trafficking ◽  
Hippocampal Neurons ◽  
Surface Membrane ◽  
Cell Types ◽  
Homogeneous Surface ◽  
Hek Cells ◽  
Membrane Protein Trafficking

Voltage-gated K+ (Kv) channels regulate membrane potential in many cell types. Although the channel surface density and location must be well controlled, little is known about Kv channel delivery and retrieval on the cell surface. The Kv2.1 channel localizes to micron-sized clusters in neurons and transfected human embryonic kidney (HEK) cells, where it is nonconducting. Because Kv2.1 is postulated to be involved in soluble N-ethylmaleimide–sensitive factor attachment protein receptor–mediated membrane fusion, we examined the hypothesis that these surface clusters are specialized platforms involved in membrane protein trafficking. Total internal reflection–based fluorescence recovery after photobleaching studies and quantum dot imaging of single Kv2.1 channels revealed that Kv2.1-containing vesicles deliver cargo at the Kv2.1 surface clusters in both transfected HEK cells and hippocampal neurons. More than 85% of cytoplasmic and recycling Kv2.1 channels was delivered to the cell surface at the cluster perimeter in both cell types. At least 85% of recycling Kv1.4, which, unlike Kv2.1, has a homogeneous surface distribution, is also delivered here. Actin depolymerization resulted in Kv2.1 exocytosis at cluster-free surface membrane. These results indicate that one nonconducting function of Kv2.1 is to form microdomains involved in membrane protein trafficking. This study is the first to identify stable cell surface platforms involved in ion channel trafficking.

scholarly journals Chronic PKC Activation Inhibits the Repolarizing Cardiac Current by Decreasing the Functional ion Channel Expression at the Plasma Membrane

2011 ◽  
Vol 100 (3) ◽  
pp. 284a
Author(s):  
Kaleef Williams ◽  
Jin O-Uchi ◽  
Angelica Martinez Perez ◽  
Coeli M.B. Lopes
Keyword(s):  
Plasma Membrane ◽  
Ion Channel ◽  
Channel Expression ◽  
Pkc Activation
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