scholarly journals A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition

2017 ◽  
Vol 106 ◽  
pp. 74-84 ◽  
Author(s):  
Kirsty E. Clarke ◽  
Daniel M. Tams ◽  
Andrew P. Henderson ◽  
Mathilde F. Roger ◽  
Andrew Whiting ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neurite Outgrowth ◽  
Culture System ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cell ◽  
Three Dimensional Culture

scholarly journals Three-dimensional culture and cAMP signaling promote the maturation of human pluripotent stem cell-derived hepatocytes

Development ◽  
2013 ◽  
Vol 140 (15) ◽  
pp. 3285-3296 ◽  
Author(s):  
S. Ogawa ◽  
J. Surapisitchat ◽  
C. Virtanen ◽  
M. Ogawa ◽  
M. Niapour ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Camp Signaling ◽  
Human Pluripotent Stem Cell ◽  
Three Dimensional Culture

Culture optimization of muscle stem cell using bead-based three-dimensional culture system

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S93
Author(s):  
S. Lee ◽  
K. Kim ◽  
S. Park ◽  
S. Lee ◽  
H. Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Culture System ◽  
Three Dimensional ◽  
Muscle Stem Cell ◽  
Culture Optimization ◽  
Three Dimensional Culture

scholarly journals A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production

2014 ◽  
Vol 2 (5) ◽  
pp. 734-745 ◽  
Author(s):  
Tomomi G. Otsuji ◽  
Jiang Bin ◽  
Azumi Yoshimura ◽  
Misayo Tomura ◽  
Daiki Tateyama ◽  
...  
Keyword(s):  
Stem Cell ◽  
Culture System ◽  
Pluripotent Stem Cell ◽  
Large Scale ◽  
Functional Polymers ◽  
Human Pluripotent Stem Cell ◽  
Cell Production ◽  
Sphere Culture

Convergence of human pluripotent stem cell, organoid, and genome editing technologies

2021 ◽  
pp. 153537022098580
Author(s):  
Lin Wang ◽  
Zhaohui Ye ◽  
Yoon-Young Jang
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Genome Editing ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Experimental Models ◽  
Human Pluripotent Stem Cell ◽  
Research Areas ◽  
Technological Advances

The last decade has seen many exciting technological breakthroughs that greatly expanded the toolboxes for biological and biomedical research, yet few have had more impact than induced pluripotent stem cells and modern-day genome editing. These technologies are providing unprecedented opportunities to improve physiological relevance of experimental models, further our understanding of developmental processes, and develop novel therapies. One of the research areas that benefit greatly from these technological advances is the three-dimensional human organoid culture systems that resemble human tissues morphologically and physiologically. Here we summarize the development of human pluripotent stem cells and their differentiation through organoid formation. We further discuss how genetic modifications, genome editing in particular, were applied to answer basic biological and biomedical questions using organoid cultures of both somatic and pluripotent stem cell origins. Finally, we discuss the potential challenges of applying human pluripotent stem cell and organoid technologies for safety and efficiency evaluation of emerging genome editing tools.

scholarly journals Human Pluripotent Stem Cell-Derived Cardiac Cells: Application in Disease Modeling, Cell Therapy, and Drug Discovery

2021 ◽  
Vol 9 ◽  
Author(s):  
Juan Huang ◽  
Qi Feng ◽  
Li Wang ◽  
Bingying Zhou
Keyword(s):  
Stem Cell ◽  
Drug Discovery ◽  
Cell Therapy ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Three Dimensional ◽  
Cardiac Cells ◽  
Cardiac Diseases ◽  
Human Pluripotent Stem Cell ◽  
Species Specific

Cardiac diseases are the leading cause of deaths worldwide; however, to date, there has been limited progress in the development of therapeutic options for these conditions. Animal models have been the most extensively studied methods to recapitulate a wide variety of cardiac diseases, but these models exhibit species-specific differences in physiology, metabolism and genetics, which lead to inaccurate and unpredictable drug safety and efficacy results, resulting in drug attrition. The development of human pluripotent stem cell (hPSC) technology in theory guarantees an unlimited source of human cardiac cells. These hPSC-derived cells are not only well suited for traditional two-dimensional (2-D) monoculture, but also applicable to more complex systems, such as three-dimensional (3-D) organoids, tissue engineering and heart on-a-chip. In this review, we discuss the application of hPSCs in heart disease modeling, cell therapy, and next-generation drug discovery. While the hPSC-related technologies still require optimization, their advances hold promise for revolutionizing cell-based therapies and drug discovery.

scholarly journals Cardiac Tissues From Stem Cells

2021 ◽  
Vol 128 (6) ◽  
pp. 775-801
Author(s):  
Giulia Campostrini ◽  
Laura M. Windt ◽  
Berend J. van Meer ◽  
Milena Bellin ◽  
Christine L. Mummery
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Three Dimensional ◽  
Cell Types ◽  
The Body ◽  
Current Status ◽  
Human Pluripotent Stem Cell ◽  
3 Dimensional

The ability of human pluripotent stem cells to form all cells of the body has provided many opportunities to study disease and produce cells that can be used for therapy in regenerative medicine. Even though beating cardiomyocytes were among the first cell types to be differentiated from human pluripotent stem cell, cardiac applications have advanced more slowly than those, for example, for the brain, eye, and pancreas. This is, in part, because simple 2-dimensional human pluripotent stem cell cardiomyocyte cultures appear to need crucial functional cues normally present in the 3-dimensional heart structure. Recent tissue engineering approaches combined with new insights into the dialogue between noncardiomyocytes and cardiomyocytes have addressed and provided solutions to issues such as cardiomyocyte immaturity and inability to recapitulate adult heart values for features like contraction force, electrophysiology, or metabolism. Three-dimensional bioengineered heart tissues are thus poised to contribute significantly to disease modeling, drug discovery, and safety pharmacology, as well as provide new modalities for heart repair. Here, we review the current status of 3-dimensional engineered heart tissues.

scholarly journals A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76205 ◽  
Author(s):  
Sanna Vuoristo ◽  
Sanna Toivonen ◽  
Jere Weltner ◽  
Milla Mikkola ◽  
Jarkko Ustinov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Culture System ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Culture ◽  
Stem Cell Derivation ◽  
Induced Pluripotent

scholarly journals A Massive Suspension Culture System With Metabolic Purification for Human Pluripotent Stem Cell-Derived Cardiomyocytes

2014 ◽  
Vol 3 (12) ◽  
pp. 1473-1483 ◽  
Author(s):  
Natsuko Hemmi ◽  
Shugo Tohyama ◽  
Kazuaki Nakajima ◽  
Hideaki Kanazawa ◽  
Tomoyuki Suzuki ◽  
...  
Keyword(s):  
Stem Cell ◽  
Suspension Culture ◽  
Culture System ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell
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