scholarly journals Systemic pregabalin attenuates facial hypersensitivity and noxious stimulus-evoked release of glutamate in medullary dorsal horn in a rodent model of trigeminal neuropathic pain

2013 ◽  
Vol 62 (6) ◽  
pp. 831-835 ◽  
Author(s):  
Naresh Kumar ◽  
Pavel S. Cherkas ◽  
Vidya Varathan ◽  
Makiko Miyamoto ◽  
Chen Yu Chiang ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Rodent Model ◽  
Noxious Stimulus ◽  
Trigeminal Neuropathic Pain ◽  
Medullary Dorsal Horn

scholarly journals Astroglia in Medullary Dorsal Horn (Trigeminal Spinal Subnucleus Caudalis) Are Involved in Trigeminal Neuropathic Pain Mechanisms

2009 ◽  
Vol 29 (36) ◽  
pp. 11161-11171 ◽  
Author(s):  
A. Okada-Ogawa ◽  
I. Suzuki ◽  
B. J. Sessle ◽  
C.-Y. Chiang ◽  
M. W. Salter ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Pain Mechanisms ◽  
Trigeminal Neuropathic Pain ◽  
Medullary Dorsal Horn

Endomorphin-1 and Endomorphin-2 Modulate Responses of Trigeminal Neurons Evoked by N-Methyl-d-Aspartic Acid and Somatosensory Stimuli

2000 ◽  
Vol 83 (6) ◽  
pp. 3570-3574 ◽  
Author(s):  
Xiao-Min Wang ◽  
Kai-Ming Zhang ◽  
Layron O. Long ◽  
Carmina A. Flores ◽  
Sukhbir S. Mokha
Keyword(s):  
Dorsal Horn ◽  
Opioid Receptor ◽  
Inhibitory Effect ◽  
Noxious Stimulus ◽  
Evoked Responses ◽  
Natural Stimulus ◽  
Nociceptive Neurons ◽  
Medullary Dorsal Horn ◽  
Trigeminal Neurons ◽  
Μ Opioid Receptor

The present study investigated the modulation of N-methyl-d-aspartate (NMDA)-evoked and peripheral cutaneous stimulus-evoked responses of trigeminal neurons by endomorphins, endogenous ligands for the μ-opioid receptor. Effects of endomorphins, administered microiontophoretically, were tested on the responses of nociceptive neurons recorded in the superficial and deeper dorsal horn of the medulla (trigeminal nucleus caudalis) in anesthetized rats. Endomorphin-1 and endomorphin-2 predominantly reduced the NMDA-evoked responses, producing an inhibitory effect of 54.1 ± 2.96% (mean ± SE; n = 34, P < 0.001) in 92% (34/37) of neurons and 63.6 ± 3.61% ( n = 32, P< 0.001) in 91% (32/35) of neurons, respectively. The inhibitory effect of endomorphins was modality specific; noxious stimulus-evoked responses were reduced more than nonnoxious stimulus-evoked responses. Naloxone applied at iontophoretic current that blocked the inhibitory effect of [d-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin, reduced the peak inhibitory effect of endomorphins on the NMDA- and natural stimulus-evoked responses. We suggest that endomorphins by acting at μ-opioid receptor selectively modulate noxious stimulus-evoked responses in the medullary dorsal horn.

scholarly journals Microglia and Inhibitory Circuitry in the Medullary Dorsal Horn: Laminar and Time-Dependent Changes in a Trigeminal Model of Neuropathic Pain

2021 ◽  
Author(s):  
Nuria García-Magro ◽  
Yasmina B. Martin ◽  
Pilar Negredo ◽  
Francisco Zafra ◽  
Carlos Avendaño
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Substantial Reduction ◽  
Negative Control ◽  
Infraorbital Nerve ◽  
Survival Times ◽  
Medullary Dorsal Horn ◽  
Inhibitory Circuitry ◽  
Basic Features ◽  
Heterogeneous Reduction

Craniofacial neuropathic pain affects millions of people worldwide and is often difficult to treat. Two key mechanisms underlying this condition are a loss of the negative control exerted by inhibitory interneurons and an early microglial reaction. Basic features of these mechanisms, however, are still poorly understood. Using the chronic constriction injury of the infraorbital nerve (CCI-IoN) model of neuropathic pain in mice, we have examined the changes in the expression of GAD, the synthetic enzyme of GABA, and GlyT2, the membrane transporter of glycine, as well as the microgliosis that occur at early (5 days) and late (21 days) stages post-CCI in the medullary and upper spinal dorsal horn. Our results show that CCI-IoN induces a down-regulation of GAD at both postinjury survival times, uniformly across the superficial laminae. The expression of GlyT2 showed a more discrete and heterogeneous reduction due to the basal presence in lamina III of &lsquo;patches&rsquo; of higher expression, interspersed within a less immunoreactive &lsquo;matrix&rsquo;, which showed a more substantial reduction in the expression of GlyT2. These patches coincided with foci lacking any perceptible microglial reaction, which stood out against a more diffuse areas of strong microgliosis. These findings may provide clues to better understand the neural mechanisms underlying allodynia in neuropathic pain syndromes.

scholarly journals Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

2012 ◽  
Vol 61 (8) ◽  
pp. 1276-1279 ◽  
Author(s):  
Naresh Kumar ◽  
Pavel S. Cherkas ◽  
C.Y. Chiang ◽  
Jonathan O. Dostrovsky ◽  
Barry J. Sessle ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Noxious Stimulus ◽  
Medullary Dorsal Horn ◽  
Microdialysis Study

scholarly journals An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve

2017 ◽  
Vol 18 (8) ◽  
pp. 899-907 ◽  
Author(s):  
Weihua Ding ◽  
Zerong You ◽  
Shiqian Shen ◽  
Jinsheng Yang ◽  
Grewo Lim ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Chronic Constriction Injury ◽  
Rodent Model ◽  
Infraorbital Nerve ◽  
Trigeminal Neuropathic Pain

scholarly journals Microglia and Inhibitory Circuitry in the Medullary Dorsal Horn: Laminar and Time-Dependent Changes in a Trigeminal Model of Neuropathic Pain

2021 ◽  
Vol 22 (9) ◽  
pp. 4564
Author(s):  
Nuria García-Magro ◽  
Yasmina B. Martin ◽  
Pilar Negredo ◽  
Francisco Zafra ◽  
Carlos Avendaño
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Substantial Reduction ◽  
Negative Control ◽  
Infraorbital Nerve ◽  
Survival Times ◽  
Medullary Dorsal Horn ◽  
Inhibitory Circuitry ◽  
Basic Features ◽  
Heterogeneous Reduction

Craniofacial neuropathic pain affects millions of people worldwide and is often difficult to treat. Two key mechanisms underlying this condition are a loss of the negative control exerted by inhibitory interneurons and an early microglial reaction. Basic features of these mechanisms, however, are still poorly understood. Using the chronic constriction injury of the infraorbital nerve (CCI-IoN) model of neuropathic pain in mice, we have examined the changes in the expression of GAD, the synthetic enzyme of GABA, and GlyT2, the membrane transporter of glycine, as well as the microgliosis that occur at early (5 days) and late (21 days) stages post-CCI in the medullary and upper spinal dorsal horn. Our results show that CCI-IoN induces a down-regulation of GAD at both postinjury survival times, uniformly across the superficial laminae. The expression of GlyT2 showed a more discrete and heterogeneous reduction due to the basal presence in lamina III of ‘patches’ of higher expression, interspersed within a less immunoreactive ‘matrix’, which showed a more substantial reduction in the expression of GlyT2. These patches coincided with foci lacking any perceptible microglial reaction, which stood out against a more diffuse area of strong microgliosis. These findings may provide clues to better understand the neural mechanisms underlying allodynia in neuropathic pain syndromes.

scholarly journals The grimace scale reliably assesses chronic pain in a rodent model of trigeminal neuropathic pain

2017 ◽  
Vol 2 ◽  
pp. 13-17 ◽  
Author(s):  
Titilola Akintola ◽  
Charles Raver ◽  
Paige Studlack ◽  
Olivia Uddin ◽  
Radi Masri ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Neuropathic Pain ◽  
Rodent Model ◽  
Trigeminal Neuropathic Pain
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