Localization and function of adenosine receptor subtypes at the longitudinal muscle – Myenteric plexus of the rat ileum

2011 ◽  
Vol 59 (7) ◽  
pp. 1043-1055 ◽  
Author(s):  
Cátia Vieira ◽  
Fátima Ferreirinha ◽  
Isabel Silva ◽  
Margarida Duarte-Araújo ◽  
Paulo Correia-de-Sá
Keyword(s):  
Adenosine Receptor ◽  
Myenteric Plexus ◽  
Longitudinal Muscle ◽  
Receptor Subtypes ◽  
Rat Ileum ◽  
And Function

scholarly journals Molecular probes for the human adenosine receptors

2020 ◽  
Author(s):  
Xue Yang ◽  
Laura H. Heitman ◽  
Adriaan P. IJzerman ◽  
Daan van der Es
Keyword(s):  
Adenosine Receptor ◽  
Adenosine Receptors ◽  
Molecular Probes ◽  
G Protein Coupled Receptors ◽  
Receptor Subtypes ◽  
The Past ◽  
New Chemical Entities ◽  
And Function ◽  
Receptor Modulators ◽  
G Protein Coupled

AbstractAdenosine receptors, G protein–coupled receptors (GPCRs) that are activated by the endogenous ligand adenosine, have been considered potential therapeutic targets in several disorders. To date however, only very few adenosine receptor modulators have made it to the market. Increased understanding of these receptors is required to improve the success rate of adenosine receptor drug discovery. To improve our understanding of receptor structure and function, over the past decades, a diverse array of molecular probes has been developed and applied. These probes, including radioactive or fluorescent moieties, have proven invaluable in GPCR research in general. Specifically for adenosine receptors, the development and application of covalent or reversible probes, whether radiolabeled or fluorescent, have been instrumental in the discovery of new chemical entities, the characterization and interrogation of adenosine receptor subtypes, and the study of adenosine receptor behavior in physiological and pathophysiological conditions. This review summarizes these applications, and also serves as an invitation to walk another mile to further improve probe characteristics and develop additional tags that allow the investigation of adenosine receptors and other GPCRs in even finer detail.

Neurochemical phenotype and function of endomorphin-2-containing neurons in the myenteric plexus of the rat colon

2010 ◽  
Vol 34 (8) ◽  
pp. S19-S19
Author(s):  
Jun‑Ping Li ◽  
Chang‑Jun Gao ◽  
Bo‑Chang Lü ◽  
Ting Zhang ◽  
Jiang‑Bo Ma ◽  
...  
Keyword(s):  
Myenteric Plexus ◽  
Rat Colon ◽  
And Function

scholarly journals Expression and Function of Estrogen Receptor Subtypes in Granulosa Cells: Regulation by Estradiol and Forskolin1

Endocrinology ◽  
1999 ◽  
Vol 140 (9) ◽  
pp. 4320-4334 ◽  
Author(s):  
S. Chidananda Sharma ◽  
Jeffrey W. Clemens ◽  
Margareta D. Pisarska ◽  
JoAnne S. Richards
Keyword(s):  
Estrogen Receptor ◽  
Granulosa Cells ◽  
Receptor Subtypes ◽  
And Function

scholarly journals Intestinal crypt derived enteroid coculture in presence of peristaltic longitudinal muscle myenteric plexus

2020 ◽  
Author(s):  
Daniel E Levin ◽  
Arabinda Mandal ◽  
Mark A Fleming ◽  
Katherine H Bae ◽  
Brielle Gerry ◽  
...  
Keyword(s):  
Myenteric Plexus ◽  
Longitudinal Muscle ◽  
Culture Media ◽  
Ex Vivo ◽  
Enteric Neurons ◽  
Intestinal Cell ◽  
Cell Populations ◽  
Complex Interactions ◽  
Proliferation And Differentiation ◽  
Intestinal Peristalsis

Abstract The role of enteric neurons in driving intestinal peristalsis has been known for over a century. However, in recent decades, scientists have begun to unravel additional complex interactions between this nerve plexus and other cell populations in the intestine. Investigations into these potential interactions is complicated by a paucity of tractable models of these cellular relationships. Here, we describe a novel technique for ex vivo coculture of enteroids, so called “mini-guts,” in juxtaposition to the longitudinal muscle myenteric plexus (LMMP). Key to this system, we developed a LMMP culture media that: 1) allows the LMMP to maintain ex vivo peristalsis for 2 weeks along with proliferation of neurons, glia, smooth muscle and fibroblast cells, and 2) supports the proliferation and differentiation of the intestinal stem cells into enteroids complete with epithelial enterocytes, Paneth cells, goblet cells and enteroendocrine cells. Importantly, this technique identifies a culture condition that supports both the metabolic needs of intestinal epithelium as well as neuronal elements, demonstrating the feasibility of maintaining these two populations in a single culture system. This sets the stage for experiments to better define the regulatory interactions of these two important intestinal cell populations.

Comparative pharmacology of human adenosine receptor subtypes – characterization of stably transfected receptors in CHO cells

1997 ◽  
Vol 357 (1) ◽  
pp. 1-9 ◽  
Author(s):  
K.-N. Klotz ◽  
J. Hessling ◽  
J. Hegler ◽  
C. Owman ◽  
B. Kull ◽  
...  
Keyword(s):  
Adenosine Receptor ◽  
Cho Cells ◽  
Receptor Subtypes

scholarly journals Differential targeting and function of α2A and α2C adrenergic receptor subtypes in cultured sympathetic neurons

2006 ◽  
Vol 51 (3) ◽  
pp. 397-413 ◽  
Author(s):  
Patricia C. Brum ◽  
Carl M. Hurt ◽  
Olga G. Shcherbakova ◽  
Brian Kobilka ◽  
Timothy Angelotti
Keyword(s):  
Adrenergic Receptor ◽  
Sympathetic Neurons ◽  
Receptor Subtypes ◽  
Cultured Sympathetic Neurons ◽  
And Function

Evidence for a glutamatergic neural pathway in the myenteric plexus

1991 ◽  
Vol 261 (4) ◽  
pp. G693-G700 ◽  
Author(s):  
J. W. Wiley ◽  
Y. X. Lu ◽  
C. Owyang
Keyword(s):  
Myenteric Plexus ◽  
Longitudinal Muscle ◽  
Muscle Tension ◽  
Nmda Receptor Antagonist ◽  
Aminobutyric Acid ◽  
Dependent Manner ◽  
Neural Pathway ◽  
Concentration Dependent Manner ◽  
Excitatory Neurotransmitter ◽  
Somatostatin 14

The objective of this study was to determine whether L-glutamate (L-Glu) may serve as a neurotransmitter candidate in the guinea pig myenteric plexus. We observed that [3H]Glu and gamma-[3H]aminobutyric acid were synthesized from [3H]glutamine and released from neurons of the myenteric plexus during K+ and 1,1-dimethyl-4-phenylpiperazinium-evoked depolarization in a concentration-dependent manner. Muscle tension studies performed on ileal longitudinal muscle-myenteric plexus (LM-MP) preparations revealed that L-Glu [mean effective dose (ED50) 2.5 x 10(-5) M] produced concentration-dependent contractions, which were unaffected by hexamethonium but abolished by tetrodotoxin, atropine, and magnesium, suggesting that L-Glu acts via N-methyl-D-aspartate (NMDA)-type receptors that stimulate a cholinergic neural pathway unaffected by ganglionic blockade. In addition, L-Glu (ED50 4 x 10(-5) M) and NMDA (ED50 2 x 10(-4) M) stimulated concentration-dependent release of [3H]acetylcholine (ACh) from LM-MP sections, which was inhibited by tetrodotoxin, magnesium, and the NMDA receptor antagonist D-2-amino-5-phosphonovaleric acid (AP-5). L-Glu-mediated release of [3H]ACh was enhanced by theophylline (10-6 M) and 3-isobutyl-1-methylxanthine (1 mM) and was significantly reduced by the adenylate cyclase inhibitor, 2',5'-dideoxyadenosine (10(-4) M) and somatostatin-14 (10(-6) M), which inhibits adenosine 3',5'-cyclic monophosphate (cAMP)-dependent cholinergic transmission in the myenteric plexus. These studies suggest that L-Glu may serve as an excitatory neurotransmitter in the myenteric plexus via its action on NMDA-type receptors, which are coupled to cAMP-dependent release of ACh.

Sign in / Sign up

Export Citation Format

Share Document