High-affinity choline uptake and acetylcholine-metabolizing enzymes in CNS white matter. A quantitative study

2008 ◽  
Vol 53 (6-8) ◽  
pp. 193-198 ◽  
Author(s):  
Bjørnar Hassel ◽  
Volker Solyga ◽  
Andreas Lossius
Keyword(s):  
White Matter ◽  
Quantitative Study ◽  
Choline Uptake ◽  
Metabolizing Enzymes ◽  
High Affinity

Effects of ovariectomy and estradiol benzoate on high affinity choline uptake, ACh synthesis, and release from rat cerebral cortical synaptosomes

1987 ◽  
Vol 403 (2) ◽  
pp. 389-392 ◽  
Author(s):  
Cecilia A. O'Malley ◽  
R. Dean Hautamaki ◽  
Mark Kelley ◽  
Edwin M. Meyer
Keyword(s):  
Estradiol Benzoate ◽  
Choline Uptake ◽  
High Affinity

Mapping of the distribution of high affinity choline uptake and choline acetyltransferase in the striatum

1980 ◽  
Vol 194 (2) ◽  
pp. 583-587 ◽  
Author(s):  
Yukio Takano ◽  
Yoshiro Kohjimoto ◽  
Kouchi Uchimura ◽  
Hiro-o Kamiya
Keyword(s):  
Choline Acetyltransferase ◽  
Choline Uptake ◽  
High Affinity

scholarly journals Choline Uptake in Agrobacterium tumefaciens by the High-Affinity ChoXWV Transporter

2011 ◽  
Vol 193 (19) ◽  
pp. 5119-5129 ◽  
Author(s):  
M. Aktas ◽  
K. A. Jost ◽  
C. Fritz ◽  
F. Narberhaus
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Choline Uptake ◽  
High Affinity

scholarly journals Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Chen Zhao ◽  
Mengjuan Lin ◽  
Yasi Pan ◽  
Baoping Yu
Keyword(s):  
Normal Saline ◽  
Specific Inhibitor ◽  
Visceral Hypersensitivity ◽  
Choline Uptake ◽  
Uptake System ◽  
Threshold Intensity ◽  
Choline Transporter ◽  
High Affinity ◽  
Acetylcholine Concentration ◽  
High Affinity Choline Transporter

Background. Visceral hypersensitivity is a common feature of irritable bowel syndrome. Cholinergic system involves in the development of visceral hypersensitivity, and high-affinity choline transporter (CHT1) is of crucial importance in choline uptake system. However, involvement of CHT1 in visceral hypersensitivity remains unknown. The research aimed to study the CHT1 expression in dorsal root ganglions (DRGs) and the role of CHT1 in visceral hypersensitivity. Methods. Repetitive water avoidance stress (WAS) was used to induce visceral hypersensitivity in rats. Colorectal distension (CRD) was determined, and the abdominal withdrawal reflex (AWR) and threshold intensity data were recorded to measure the visceral sensitivity. After intraperitoneal injection of hemicholinium-3 (HC-3), the specific inhibitor of CHT1, CRD data were also recorded. The CHT1 expression of DRGs was investigated by Western blotting, immunohistochemistry, and quantitative RT-PCR. Acetylcholine levels in the DRGs were detected by the assay kit. Results. Repetitive WAS increased the AWR score of CRD at high distension pressure and decreased the mean threshold of rats. The CHT1 expression and acetylcholine concentration of DRG were significantly increased in WAS rats. After the administration of HC-3, the AWR score in WAS group was significantly increased at higher distension pressure while the threshold intensity was significantly reduced compared to the normal saline group. Acetylcholine concentration was significantly lower than the normal saline rats. Conclusion. Our research firstly reports that CHT1 is overexpressed in noninflammatory visceral hypersensitivity, and blockage of CHT1 can enhance the visceral hypersensitivity. CHT1 may play an inhibitory role in visceral hypersensitivity.

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