Modulation of Cav1 and Cav2.2 channels induced by nitric oxide via cGMP-dependent protein kinase

2004 ◽  
Vol 45 (6) ◽  
pp. 885-893 ◽  
Author(s):  
Claudio Grassi ◽  
Marcello D’Ascenzo ◽  
Gian Battista Azzena
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals TREK-1 Regulation by Nitric Oxide and cGMP-dependent Protein Kinase

2001 ◽  
Vol 276 (47) ◽  
pp. 44338-44346 ◽  
Author(s):  
Sang Don Koh ◽  
Kevin Monaghan ◽  
Gerard P. Sergeant ◽  
Seungil Ro ◽  
Rebecca L. Walker ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Testosterone-induced relaxation of coronary arteries: activation of BKCa channels via the cGMP-dependent protein kinase

2012 ◽  
Vol 302 (1) ◽  
pp. H115-H123 ◽  
Author(s):  
Viju Deenadayalu ◽  
Yashoda Puttabyatappa ◽  
Alexander T. Liu ◽  
John N. Stallone ◽  
Richard E. White
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Protein Kinase ◽  
Coronary Arteries ◽  
Specific Inhibitor ◽  
No Production ◽  
Channel Activity ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein

Androgens are reported to have both beneficial and detrimental effects on human cardiovascular health. The aim of this study was to characterize nongenomic signaling mechanisms in coronary artery smooth muscle (CASM) and define the ionic basis of testosterone (TES) action. TES-induced relaxation of endothelium-denuded porcine coronary arteries was nearly abolished by 20 nM iberiotoxin, a highly specific inhibitor of large-conductance, calcium-activated potassium (BKCa) channels. Molecular patch-clamp studies confirmed that nanomolar concentrations of TES stimulated BKCa channel activity by ∼100-fold and that inhibition of nitric oxide synthase (NOS) activity by NG-monomethyl-l-arginine nearly abolished this effect. Inhibition of nitric oxide (NO) synthesis or guanylyl cyclase activity also attenuated TES-induced coronary artery relaxation but did not alter relaxation due to 8-bromo-cGMP. Furthermore, we detected TES-stimulated NO production in porcine coronary arteries and in human CASM cells via stimulation of the type 1 neuronal NOS isoform. Inhibition of the cGMP-dependent protein kinase (PKG) attenuated TES-stimulated BKCa channel activity, and direct assay determined that TES increased activity of PKG in a concentration-dependent fashion. Last, the stimulatory effect of TES on BKCa channel activity was mimicked by addition of purified PKG to the cytoplasmic surface of a cell-free membrane patch from CASM myocytes (∼100-fold increase). These findings indicate that TES-induced relaxation of endothelium-denuded coronary arteries is mediated, at least in part, by enhanced NO production, leading to cGMP synthesis and PKG activation, which, in turn, opens BKCa channels. These findings provide a molecular mechanism that could help explain why androgens have been reported to relax coronary arteries and relieve angina pectoris.

scholarly journals Relaxin Affects Smooth Muscle Biophysical Properties and Mechanical Activity of the Female Mouse Colon

Endocrinology ◽  
2015 ◽  
Vol 156 (12) ◽  
pp. 4398-4410 ◽  
Author(s):  
Roberta Squecco ◽  
Rachele Garella ◽  
Eglantina Idrizaj ◽  
Silvia Nistri ◽  
Fabio Francini ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Protein Kinase ◽  
Guanylate Cyclase ◽  
Membrane Properties ◽  
Mechanical Activity ◽  
Biophysical Properties ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein

The hormone relaxin (RLX) has been reported to influence gastrointestinal motility in mice. However, at present, nothing is known about the effects of RLX on the biophysical properties of the gastrointestinal smooth muscle cells (SMCs). Other than extending previous knowledge of RLX on colonic motility, the purpose of this study was to investigate the ability of the hormone to induce changes in resting membrane potential (RMP) and on sarcolemmal ion channels of colonic SMCs of mice that are related to its mechanical activity. To this aim, we used a combined mechanical and electrophysiological approach. In the mechanical experiments, we observed that RLX caused a decay of the basal tone coupled to an increase of the spontaneous contractions, completely abolished by the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one (ODQ). The electrophysiological results indicate for the first time that RLX directly affects the SMC biophysical properties inducing hyperpolarization of RMP and cycles of slow hyperpolarization/depolarization oscillations. The effects of RLX on RMP were abolished by ODQ as well as by a specific inhibitor of the cGMP-dependent protein kinase, KT5823. RLX reduced Ca2+ entry through the voltage-dependent L-type channels and modulated either voltage- or ATP-dependent K+ channels. These effects were abolished by ODQ, suggesting the involvement of the nitric oxide/guanylate cyclase pathway in the effects of RLX on RMP and ion channel modulation. These actions of RLX on membrane properties may contribute to the regulation of the proximal colon motility by the nitric oxide/cGMP/cGMP-dependent protein kinase pathway.

scholarly journals Gene Transfer of cGMP-Dependent Protein Kinase I Enhances the Antihypertrophic Effects of Nitric Oxide in Cardiomyocytes

Hypertension ◽  
2002 ◽  
Vol 39 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Kai C. Wollert ◽  
Beate Fiedler ◽  
Stepan Gambaryan ◽  
Albert Smolenski ◽  
Jörg Heineke ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Gene Transfer ◽  
Dependent Protein Kinase ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein
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