The tyrosine phosphatase inhibitor orthovanadate mimics NGF-induced neuroprotective signaling in rat hippocampal neurons

2004 ◽  
Vol 44 (7) ◽  
pp. 505-520 ◽  
Author(s):  
Norbert Gerling ◽  
Carsten Culmsee ◽  
Susanne Klumpp ◽  
Josef Krieglstein
Keyword(s):  
Hippocampal Neurons ◽  
Tyrosine Phosphatase ◽  
Phosphatase Inhibitor ◽  
Neuroprotective Signaling

Evidence that tyrosine phosphorylation may increase tight junction permeability

1995 ◽  
Vol 108 (2) ◽  
pp. 609-619 ◽  
Author(s):  
J.M. Staddon ◽  
K. Herrenknecht ◽  
C. Smales ◽  
L.L. Rubin
Keyword(s):  
Endothelial Cells ◽  
Tight Junction ◽  
Tyrosine Phosphorylation ◽  
Mdck Cells ◽  
Tyrosine Phosphatase ◽  
Adherens Junction ◽  
Brain Endothelial Cells ◽  
Phenylarsine Oxide ◽  
Phosphatase Inhibitor ◽  
Tight Junction Permeability

Tight junction permeability control is important in a variety of physiological and pathological processes. We have investigated the role of tyrosine phosphorylation in the regulation of tight junction permeability. MDCK epithelial cells and brain endothelial cells were grown on filters and tight junction permeability was determined by transcellular electrical resistance (TER). The tyrosine phosphatase inhibitor pervanadate caused a concentration- and time-dependent decrease in TER in both MDCK and brain endothelial cells. However, as expected, pervanadate resulted in the tyrosine phosphorylation of many proteins; hence interpretation of its effects are extremely difficult. Phenylarsine oxide, a more selective tyrosine phosphatase inhibitor, caused the tyrosine phosphorylation of relatively few proteins as analyzed by immunoblotting of whole cell lysates. This inhibitor, like pervanadate, also elicited a decrease in TER in the two cell types. In the MDCK cells, the action of phenylarsine oxide could be reversed by the subsequent addition of the reducing agent 2,3-dimercaptopropanol. Immunocytochemistry revealed that phenylarsine oxide rapidly stimulated the tyrosine phosphorylation of proteins associated with intercellular junctions. Because of the known influence of the adherens junction on tight junctions, we analyzed immunoprecipitates of the E-cadherin/catenin complex from MDCK cells treated with phenylarsine oxide. This revealed an increase in the tyrosine phosphorylation of beta-catenin, but not of alpha-catenin. However, the tight junction associated protein ZO-1 was also tyrosine phosphorylated after PAO treatment. These data indicate that tight junction permeability may be regulated via mechanisms involving tyrosine phosphorylation of adherens junction and tight junction proteins.

scholarly journals A protein tyrosine phosphatase inhibitor, pervanadate, inhibits angiotensin II-Induced β-arrestin cleavage

2009 ◽  
Vol 28 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Sei-Heon Jang ◽  
Si Ae Hwang ◽  
Mijin Kim ◽  
Sung-Hae Yun ◽  
Moon-Sook Kim ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine ◽  
Phosphatase Inhibitor ◽  
Protein Tyrosine Phosphatase Inhibitor

Regulation by the autophosphorylation site in overexpressed pp60c-src

1988 ◽  
Vol 8 (10) ◽  
pp. 4541-4546
Author(s):  
T E Kmiecik ◽  
P J Johnson ◽  
D Shalloway
Keyword(s):  
Kinase Activity ◽  
Tyrosine Phosphatase ◽  
Phosphatase Inhibitor ◽  
Autophosphorylation Site

We show that overexpressed pp60c-src is phosphorylated at Tyr-416 and has increased specific kinase activity when isolated from cells incubated with vanadate, a tyrosine phosphatase inhibitor. This supports the hypothesis that transient Tyr-416 phosphorylation modulates the activity of overexpressed pp60c-src in vivo. Mutagenesis indicates that Tyr-416 modulates pp60v-src activity as well.

scholarly journals Inhibitory effect of tyrosine phosphatase inhibitor on peroxynitrite-induced cell death

1998 ◽  
Vol 76 ◽  
pp. 227
Author(s):  
Makio Saeki ◽  
Sadaaki Maeda ◽  
Yasuhiro Ooi ◽  
Takashi Yamada ◽  
Tomohiko Kanesaki ◽  
...  
Keyword(s):  
Cell Death ◽  
Tyrosine Phosphatase ◽  
Inhibitory Effect ◽  
Phosphatase Inhibitor

scholarly journals Structure of the Complex of an Iminopyridinedione Protein Tyrosine Phosphatase 4A3 Phosphatase Inhibitor with Human Serum Albumin

2020 ◽  
Vol 98 (6) ◽  
pp. 648-657
Author(s):  
Mateusz P. Czub ◽  
Adam M. Boulton ◽  
Ettore J. Rastelli ◽  
Nikhil R. Tasker ◽  
Taber S. Maskrey ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine ◽  
Phosphatase Inhibitor
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