Microarray expression analysis in idiopathic and LRRK2-associated Parkinson's disease

2012 ◽  
Vol 45 (1) ◽  
pp. 462-468 ◽  
Author(s):  
Teresa Botta-Orfila ◽  
Eduard Tolosa ◽  
Ellen Gelpi ◽  
Alex Sànchez-Pla ◽  
Maria-José Martí ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Expression Analysis ◽  
Microarray Expression Analysis ◽  
Microarray Expression

Microarray expression analysis of genes and pathways involved in growth plate cartilage injury responses and bony repair

Bone ◽  
2012 ◽  
Vol 50 (5) ◽  
pp. 1081-1091 ◽  
Author(s):  
Carmen E. Macsai ◽  
Kristen R. Georgiou ◽  
Bruce K. Foster ◽  
Andrew C.W. Zannettino ◽  
Cory J. Xian
Keyword(s):  
Growth Plate ◽  
Expression Analysis ◽  
Cartilage Injury ◽  
Growth Plate Cartilage ◽  
Microarray Expression Analysis ◽  
Microarray Expression

scholarly journals Alteration of gene expression in mice after glaucoma filtration surgery

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Keisuke Adachi ◽  
Yosuke Asada ◽  
Toshiaki Hirakata ◽  
Miki Onoue ◽  
Satoshi Iwamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Marker Gene ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Microarray Expression Analysis ◽  
Epithelial Wound Healing ◽  
Microarray Expression

Abstract To clarify the early alterations of gene expression using a mouse model of glaucoma filtration surgery, we carried out microarray expression analysis. Using BALB/c mice, a filtration surgery model was made by incision of the limbal conjunctiva, followed by the insertion of a 33G needle tip into the anterior chamber, and 11-0 nylon sutures. Subgroups of mice were treated intraoperatively with 0.4 mg/ml mitomycin-C (MMC). At day 3 after surgery the bleb was maintained. The bleb region tissue was sampled 3 days after the filtration surgery, and gene expression analysis was carried out using a mouse Agilent 8 × 60 K array. We found 755 hyperexpressed transcripts in the bleb region compared to control conjunctiva. The hyperexpressed transcripts included epithelial cell metaplasia-related (Il1b, Krt16, Sprr1b), inflammation-related (Ccl2, Il6) and wound healing-related (Lox, Timp1) genes. We also found downregulation of a goblet cell marker gene (Gp2) in the bleb conjunctiva. MMC treatment suppressed elastin (Eln) gene expression and enhanced keratinization-related gene expression (Krt1, Lor) in the bleb region. Our results suggest the importance of epithelial wound healing after filtration surgery, and this filtration surgery model will be a useful tool for further pathophysiological analysis.

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