Modular nanostructured peptide materials for cell sheet regeneration

2006 ◽  
Vol 2 (4) ◽  
pp. 275
Author(s):  
J Collier
Keyword(s):  
Cell Sheet ◽  
Peptide Materials

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Biomaterials ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Tsung-Szu Yeh ◽  
Yu-Hua Dean Fang ◽  
Chia-Hsin Lu ◽  
Shao-Chieh Chiu ◽  
Chia-Lin Yeh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Sheet ◽  
Myocardium Infarction ◽  
Adipose Derived Stem Cell

scholarly journals Long-term outcomes of autologous skeletal myoblast cell-sheet transplantation for end-stage ischemic cardiomyopathy

2021 ◽  
Author(s):  
Satoshi Kainuma ◽  
Shigeru Miyagawa ◽  
Koichi Toda ◽  
Yasushi Yoshikawa ◽  
Hiroki Hata ◽  
...  
Keyword(s):  
Ischemic Cardiomyopathy ◽  
Cell Sheet ◽  
Long Term Outcomes ◽  
Skeletal Myoblast ◽  
Myoblast Cell ◽  
End Stage

Liver bioengineering: Recent trends/advances in decellularization and cell sheet technologies towards translation into the clinic

Life Sciences ◽  
2021 ◽  
pp. 119373
Author(s):  
Maryam Asadi ◽  
Mostafa Khalili ◽  
Hajie Lotfi ◽  
Sevil Vaghefi Moghaddam ◽  
Nosratollah Zarghami ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Recent Trends

Peptide Materials for Smart Therapeutic Applications

2021 ◽  
Vol 29 (1) ◽  
pp. 2-14
Author(s):  
Jeonghun Lee ◽  
Chulhee Kim
Keyword(s):  
Therapeutic Applications ◽  
Peptide Materials

scholarly journals Impact of endoscopic submucosal dissection and epithelial cell sheet engraftment on systemic cytokine dynamics in patients with oesophageal cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akira Yoshida ◽  
Tomoaki Takata ◽  
Tsutomu Kanda ◽  
Naoyuki Yamaguchi ◽  
Hitomi Minami ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Inflammatory Cytokines ◽  
Oesophageal Cancer ◽  
Cell Sheet ◽  
Major Complication ◽  
Tumour Microenvironment ◽  
Oesophageal Stricture ◽  
Submucosal Dissection ◽  
Autologous Cell ◽  
Cytokine Levels

AbstractThe tumour microenvironment (TME) plays an important role in cancer development, progression, and metastasis. Various cytokines are present in the TME in oesophageal cancer. Oesophageal stricture is a major complication of endoscopic submucosal dissection (ESD) for oesophageal cancer, and inflammatory cytokines are closely related to its pathogenesis. However, the cytokine crosstalk involved in the oesophageal cancer TME and post-ESD stricture has not been fully elucidated. This study investigated the comprehensive cytokine dynamics following ESD in patients with oesophageal cancer. In addition, the effect of a novel preventive technique for post-ESD stricture, autologous cell sheet engraftment, on cytokine levels was evaluated. Various pro-inflammatory and anti-tumorigenic cytokines were elevated in patients with oesophageal cancer, and ESD transiently influenced cytokine concentrations. IL-1β and TNF-α, two major pro-inflammatory cytokines that induce oesophageal stricture, were significantly suppressed by cell sheet engraftment. In conclusion, this study revealed the distinct cytokine dynamics after ESD in patients with oesophageal cancer, together with the effect of autologous cell sheet engraftment on cytokine fluctuation. These results can accelerate research on the TME and therapeutic strategies for oesophageal cancer.

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