scholarly journals Comparison of laser speckle contrast imaging with laser Doppler for assessing microvascular function

2011 ◽  
Vol 82 (3) ◽  
pp. 326-332 ◽  
Author(s):  
Garry A. Tew ◽  
Markos Klonizakis ◽  
Helen Crank ◽  
J. David Briers ◽  
Gary J. Hodges
Keyword(s):  
Microvascular Function ◽  
Laser Speckle ◽  
Laser Doppler ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

scholarly journals Reproducibility and normalization of reactive hyperemia using laser speckle contrast imaging

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244795
Author(s):  
Behnia Rezazadeh Shirazi ◽  
Rudy J. Valentine ◽  
James A. Lang
Keyword(s):  
Reactive Hyperemia ◽  
Intraclass Correlation ◽  
Microvascular Dysfunction ◽  
Microvascular Function ◽  
Rate Of Change ◽  
Laser Speckle ◽  
Maximal Flow ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast

Background Impaired perfusion indices signal potential microvascular dysfunction preceding atherosclerosis and other cardiometabolic pathologies. Post-occlusive reactive hyperemia (PORH), a vasodilatory response following a mechanically induced ischemia, is a transient increase in perfusion and can assess microvascular function. The greatest blood flow change corresponding to the first minute of hyperemia (represented by time-to-peak, hyperemic velocity, AUC within 1st min) has been shown to indicate microvascular dysfunction. However, the reproducibility of these temporal kinetic indices of the PORH response is unknown. Our aim was to examine the inter- and intra-day reproducibility and standardization of reactive hyperemia, with emphasis on the kinetic indices of PORH, using laser speckle contrast imaging (LSCI) technique. Methods and results Seventeen healthy adults (age = 24 ± 3 years) completed three PORH bouts over two lab visits. LSCI region of interest was a standardized 10 cm region on the dominant ventral forearm. A 5-min brachial artery occlusion period induced by inflating an arm cuff to 200 mmHg, preceded a 4-min hyperemic period. Inter- and intra-day reliability and reproducibility of cutaneous vascular conductance (LSCI flux / mean arterial pressure) were determined using intraclass correlation (ICC) and coefficient of variation (CV%). Maximal flow and area under the curve standardized to zero perfusion showed intra- and inter-day reliability (ICC > 0.70). Time to maximal flow (TMF) was not reproducible (inter-day CV = 18%). However, alternative kinetic indices such as 1-min AUC and overshoot rate-of-change (ORC), represented as a piecewise function (at 5s, 10s, 15s, and 20s into hyperemia), were reproducible (CV< 11%). Biological zero was a reliable normalization point. Conclusion PORH measured with LSCI is a reliable assessment of microvascular function. However, TMF or its derived hyperemic velocity are not recommended for longitudinal assessment. Piecewise ORC and 1-min AUC are reliable alternatives to assess the kinetic response of PORH.

Comparison of laser speckle contrast imaging and laser-Doppler fluxmetry in boys and men

2020 ◽  
Vol 128 ◽  
pp. 103927
Author(s):  
Gary J. Hodges ◽  
Panagiota Klentrou ◽  
Stephen S. Cheung ◽  
Bareket Falk
Keyword(s):  
Laser Speckle ◽  
Laser Doppler ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Laser Doppler Fluxmetry

scholarly journals Performance of noninvasive laser Doppler flowmetry and laser speckle contrast imaging methods in diagnosis of Buerger disease

Medicine ◽  
2018 ◽  
Vol 97 (43) ◽  
pp. e12979
Author(s):  
Loukman Omarjee ◽  
Antoine Larralde ◽  
Vincent Jaquinandi ◽  
Olivier Stivalet ◽  
Guillaume Mahe
Keyword(s):  
Laser Doppler Flowmetry ◽  
Laser Speckle ◽  
Laser Doppler ◽  
Contrast Imaging ◽  
Doppler Flowmetry ◽  
Laser Speckle Contrast Imaging ◽  
Imaging Methods ◽  
Speckle Contrast

scholarly journals Use of Laser Speckle Contrast Imaging to Assess Digital Microvascular Function in Primary Raynaud Phenomenon and Systemic Sclerosis: A Comparison Using the Raynaud Condition Score Diary

2015 ◽  
Vol 42 (7) ◽  
pp. 1163-1168 ◽  
Author(s):  
John D. Pauling ◽  
Jacqueline A. Shipley ◽  
Darren J. Hart ◽  
Anita McGrogan ◽  
Neil J. McHugh
Keyword(s):  
Systemic Sclerosis ◽  
Objective Assessment ◽  
Microvascular Function ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Raynaud Phenomenon ◽  
Laser Speckle Contrast Imaging ◽  
Speckle Contrast ◽  
Condition Score ◽  
Cold Challenge

Objective.Evaluate objective assessment of digital microvascular function using laser speckle contrast imaging (LSCI) in a cross-sectional study of patients with primary Raynaud phenomenon (RP) and systemic sclerosis (SSc), comparing LSCI with both infrared thermography (IRT) and subjective assessment using the Raynaud Condition Score (RCS) diary.Methods.Patients with SSc (n = 25) and primary RP (n = 18) underwent simultaneous assessment of digital perfusion using LSCI and IRT with a cold challenge on 2 occasions, 2 weeks apart. The RCS diary was completed between assessments. The relationship between objective and subjective assessments of RP was evaluated. Reproducibility of LSCI/IRT was assessed, along with differences between primary RP and SSc, and the effect of sex.Results.There was moderate-to-good correlation between LSCI and IRT (Spearman rho 0.58–0.84, p < 0.01), but poor correlation between objective assessments and the RCS diary (p > 0.05 for all analyses). Reproducibility of IRT and LSCI was moderate at baseline (ICC 0.51–0.63) and immediately following cold challenge (ICC 0.56–0.86), but lower during reperfusion (ICC 0.3–0.7). Neither subjective nor objective assessments differentiated between primary RP and SSc. Men reported lower median daily frequency of RP attacks (0.82 vs 1.93, p = 0.03). Perfusion using LSCI/IRT was higher in men for the majority of assessments.Conclusion.Objective and subjective methods provide differing information on microvascular function in RP. There is good convergent validity of LSCI with IRT and acceptable reproducibility of both modalities. Neither subjective nor objective assessments could differentiate between primary RP and SSc. Influence of sex on subjective and objective assessment of RP warrants further evaluation.

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