Characterization of microvascular endothelial cells isolated from the dermis of adult mouse tails

2011 ◽  
Vol 82 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Dodanim Talavera-Adame ◽  
Tina T. Ng ◽  
Ankur Gupta ◽  
Silvia Kurtovic ◽  
Gordon D. Wu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adult Mouse ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

Nucleoside and nucleobase transporters of primary human cardiac microvascular endothelial cells: characterization of a novel nucleobase transporter

2007 ◽  
Vol 293 (6) ◽  
pp. H3325-H3332 ◽  
Author(s):  
Derek B. J. Bone ◽  
James R. Hammond
Keyword(s):  
Endothelial Cells ◽  
Cell Types ◽  
Microvascular Endothelial Cells ◽  
Nucleoside Transporter ◽  
Active Metabolites ◽  
Equilibrative Nucleoside Transporter ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells ◽  
Nucleobase Transport

Levels of cardiovascular active metabolites, like adenosine, are regulated by nucleoside transporters of endothelial cells. We characterized the nucleoside and nucleobase transport capabilities of primary human cardiac microvascular endothelial cells (hMVECs). hMVECs accumulated 2-[3H]chloroadenosine via the nitrobenzylmercaptopurine riboside-sensitive equilibrative nucleoside transporter 1 (ENT1) at a Vmaxof 3.4 ± 1 pmol·μl−1·s−1, with no contribution from the nitrobenzylmercaptopurine riboside-insensitive ENT2. Inhibition of 2-chloroadenosine uptake by ENT1 blockers produced monophasic inhibition curves, which are also compatible with minimal ENT2 expression. The nucleobase [3H]hypoxanthine was accumulated within hMVECs ( Km= 96 ± 37 μM; Vmax= 1.6 ± 0.3 pmol·μl−1·s−1) despite the lack of a known nucleobase transport system. This novel transporter was dipyridamole-insensitive but could be inhibited by adenine ( Ki= 19 ± 7 μM) and other purine nucleobases, including chemotherapeutic analogs. A variety of other cell types also expressed the nucleobase transporter, including the nucleoside transporter-deficient PK( 15 ) cell line (PK15NTD). Further characterization of [3H]hypoxanthine uptake in the PK15NTD cells showed no dependence on Na+or H+. PK15NTD cells expressing human ENT2 accumulated 4.5-fold more [3H]hypoxanthine in the presence of the ENT2 inhibitor dipyridamole than did PK15NTD cells or hMVECs, suggesting trapping of ENT2-permeable metabolites. Understanding the nucleoside and nucleobase transporter profiles in the vasculature will allow for further study into their roles in pathophysiological conditions such as hypoxia or ischemia.

Characterization of adhesion receptors on cultured microvascular endothelial cells derived from the retroorbital connective tissue of patients with Graves' ophthalmopathy

1996 ◽  
Vol 134 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Armin E Heufelder ◽  
Peter C Scriba
Keyword(s):  
T Cells ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Connective Tissue ◽  
Adhesion Molecule ◽  
Microvascular Endothelial Cells ◽  
Cell Populations ◽  
Graves Ophthalmopathy ◽  
Microvascular Endothelial

Heufelder AE, Scriba PC. Characterization of adhesion receptors on cultured microvascular endothelial cells derived from the retroorbital connective tissue of patients with Graves' ophthalmopathy. Eur J Endocrinol 1996:134:51–60 T lymphocytes have been demonstrated recently to play an important role in the pathogenesis and propagation of Graves' ophthalmopathy (GO). Recruitment of T cells to the retroorbital tissue in GO involves the activation of certain adhesion molecules both in the vascular endothelium and in the extravascular connective tissue within the retroorbital space. To characterize the interactions between orbital endothelial cells (OECs) and circulating T cells in vitro, we designed a two-step immunopurification procedure with bead-immobilized Ulex europaeus I lectin and anti-human endothelial cell antigen (CD3I) monoclonal antibody for rapid and reproducible isolation of highly pure microvascular endothelial cell populations from small quantities of retroorbital connective tissue. Endothelial origin of the resulting cell populations was confirmed by positive immunoreactivity for von Willebrand factor. CD 3 I and thrombomodulin. Under baseline conditions, GO-OECs, but not normal OECs, expressed intercellular adhesion molecule 1 (ICAM-1) and CD44 immunoreactivity but no immunoreactivity for endothelial leukocyte adhesion molecule I (ELAM-1) and vascular cell adhesion molecule I (VCAM-1) was detected. Exposure of GO-OEC and normal OEC monolayers to interferon γ, interleukin 1 α and tumor necrosis factor α resulted in marked up-regulation of immunoreactivity for ICAM-1 and in induction of ELAM-1 and VCAM-1. Blocking experiments using monoclonal antibodies directed against various adhesion molecules demonstrated that interactions between matched activated T lymphocytes and OECs were mediated by integrin-dependent ICAM-1/leukocyte function-associated antigen 1 (LFA-1): VCAM-1/very late antigen 4 (VLA-4)) and integrin-independent (CD44) pathways, and revealed marked differences when comparing GO-OECs and normal OECs. In conclusion, the availability of OECs from affected retroorbital tissue of patients with GO provides a valuable tool for studying further the mechanisms responsible for orbit-specific lymphocyte recruitment in GO. Armin E Heufelder, Molecular Thyroid Research Unit, Medizinische Klinik, Klinikum Innenstadt, Ziemssenstrasse 1, 80336 München, Germany

Isolation and Characterization of Fetus Human Retinal Microvascular Endothelial Cells

2010 ◽  
Vol 44 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Zhang Xiaozhuang ◽  
Luo Xianqiong ◽  
Jiang Jingbo ◽  
Huang Shuiqing ◽  
Yan Jie ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Isolation And Characterization ◽  
Microvascular Endothelial

Long-term culture and characterization of human limbal microvascular endothelial cells

1990 ◽  
Vol 51 (6) ◽  
pp. 645-650 ◽  
Author(s):  
François Marceau ◽  
Hélène M. Boisjoly ◽  
Eric Wagner ◽  
Simone Lille ◽  
Raynald Roy
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Long Term Culture ◽  
Microvascular Endothelial

scholarly journals Characterization of immortalized human dermal microvascular endothelial cells (HMEC-1) for the study of HDL functionality

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Mónica Muñoz-Vega ◽  
Felipe Massó ◽  
Araceli Páez ◽  
Elizabeth Carreón-Torres ◽  
Hector A. Cabrera-Fuentes ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Hdl Functionality ◽  
Microvascular Endothelial

scholarly journals Immunoelectron Microscopic Characterization of Human Dermal Lymphatic Microvascular Endothelial Cells: Differential Expression of CD31, CD34, and Type IV Collagen with Lymphatic Endothelial Cells vs Blood Capillary Endothelial Cells in Normal Human Skin, Lymphangioma, and Hemangioma In Situ

1998 ◽  
Vol 46 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Birthe Sauter ◽  
Dagmar Foedinger ◽  
Barbara Sterniczky ◽  
Klaus Wolff ◽  
Klemens Rappersberger
Keyword(s):  
Endothelial Cells ◽  
Human Skin ◽  
Type Iv Collagen ◽  
Microvascular Endothelial Cells ◽  
Lymphatic Endothelial Cells ◽  
Normal Human Skin ◽  
Type Iv ◽  
Normal Human ◽  
Microvascular Endothelial

We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial cells. With blood microvascular endothelial cells, we observed uniform labeling of the luminal cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial cells in normal human skin and in lymphangiomas displayed, in addition to a luminal labeling, pronounced expression of CD31 and CD34 along the abluminal cell membranes. Moreover, CD31 was preferentially detected within intercellular junctions. The expression of CD34 was mostly confined to abluminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial cells.

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