Highly-defined bioprinting of long-term vascularized scaffolds with Bio-Trap: Complex geometry functionalization and process parameters with computer aided tissue engineering

Materialia ◽  
2020 ◽  
Vol 9 ◽  
pp. 100560 ◽  
Author(s):  
Ruben Foresti ◽  
Stefano Rossi ◽  
Silvana Pinelli ◽  
Rossella Alinovi ◽  
Matteo Barozzi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Process Parameters ◽  
Complex Geometry ◽  
Computer Aided

3D Printing & Pharmaceutical Manufacturing: Opportunities and Challenges

2016 ◽  
Vol 5 (01) ◽  
pp. 4723 ◽  
Author(s):  
Bhusnure O. G.* ◽  
Gholve V. S. ◽  
Sugave B. K. ◽  
Dongre R. C. ◽  
Gore S. A. ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
3D Printing ◽  
Computer Aided Design ◽  
Patient Specific ◽  
Computer Design ◽  
3D Scaffolds ◽  
Engineering Research ◽  
Advantages And Disadvantages ◽  
Computer Aided

Many researchers have attempted to use computer-aided design (C.A.D) and computer-aided manufacturing (CAM) to realize a scaffold that provides a three-dimensional (3D) environment for regeneration of tissues and organs. As a result, several 3D printing technologies, including stereolithography, deposition modeling, inkjet-based printing and selective laser sintering have been developed. Because these 3D printing technologies use computers for design and fabrication, and they can fabricate 3D scaffolds as designed; as a consequence, they can be standardized. Growth of target tissues and organs requires the presence of appropriate growth factors, so fabrication of 3Dscaffold systems that release these biomolecules has been explored. A drug delivery system (D.D.S) that administrates a pharmaceutical compound to achieve a therapeutic effect in cells, animals and humans is a key technology that delivers biomolecules without side effects caused by excessive doses. 3D printing technologies and D. D. Ss have been assembled successfully, so new possibilities for improved tissue regeneration have been suggested. If the interaction between cells and scaffold system with biomolecules can be understood and controlled, and if an optimal 3D tissue regenerating environment is realized, 3D printing technologies will become an important aspect of tissue engineering research in the near future. 3D Printing promises to produce complex biomedical devices according to computer design using patient-specific anatomical data. Since its initial use as pre-surgical visualization models and tooling molds, 3D Printing has slowly evolved to create one-of-a-kind devices, implants, scaffolds for tissue engineering, diagnostic platforms, and drug delivery systems. Fuelled by the recent explosion in public interest and access to affordable printers, there is renewed interest to combine stem cells with custom 3D scaffolds for personalized regenerative medicine. Before 3D Printing can be used routinely for the regeneration of complex tissues (e.g. bone, cartilage, muscles, vessels, nerves in the craniomaxillofacial complex), and complex organs with intricate 3D microarchitecture (e.g. liver, lymphoid organs), several technological limitations must be addressed. Until recently, tablet designs had been restricted to the relatively small number of shapes that are easily achievable using traditional manufacturing methods. As 3D printing capabilities develop further, safety and regulatory concerns are addressed and the cost of the technology falls, contract manufacturers and pharmaceutical companies that experiment with these 3D printing innovations are likely to gain a competitive edge. This review compose the basics, types & techniques used, advantages and disadvantages of 3D printing

scholarly journals Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen

Gels ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 44
Author(s):  
Marfa N. Egorikhina ◽  
Yulia P. Rubtsova ◽  
Diana Ya. Aleynik
Keyword(s):  
Tissue Engineering ◽  
Proliferative Activity ◽  
Experimental Protocol ◽  
Hydrogel Scaffold ◽  
Hybrid Hydrogel ◽  
Hydrogel Scaffolds ◽  
High Viability ◽  
Scaffold Structure ◽  
Difficult Issue

The most difficult issue when using tissue engineering products is enabling the ability to store them without losing their restorative capacity. The numbers and viability of mesenchymal stem cells encapsulated in a hydrogel scaffold after cryostorage at −80 °C (by using, individually, two kinds of cryoprotectors—Bambanker and 10% DMSO (Dimethyl sulfoxide) solution) for 3, 6, 9, and 12 months were determined, with subsequent assessment of cell proliferation after 96 h. The analysis of the cellular component was performed using fluorescence microscopy and the two fluorochromes—Hoechst 3334 and NucGreenTM Dead 488. The experimental protocol ensured the preservation of cells in the scaffold structure, retaining both high viability and proliferative activity during storage for 3 months. Longer storage of scaffolds led to their significant changes. Therefore, after 6 months, the proliferative activity of cells decreased. Cryostorage of scaffolds for 9 months led to a decrease in cells’ viability and proliferative activity. As a result of cryostorage of scaffolds for 12 months, a decrease in viability and proliferative activity of cells was observed, as well as pronounced changes in the structure of the hydrogel. The described scaffold cryostorage protocol could become the basis for the development of storage protocols for such tissue engineering products, and for helping to extend the possibilities of their clinical use while accelerating their commercialization.

A Paradigm for Functional Tissue Engineering

2000 ◽  
Author(s):  
David L. Butler
Keyword(s):  
Tissue Engineering ◽  
Tendon Repair ◽  
National Committee ◽  
New Paradigm ◽  
Functional Tissue Engineering ◽  
Functional Tissue ◽  
Tissue Engineer ◽  
Surgical Implants ◽  
The Us

Abstract Clinicians, biologists, and engineers face difficult challenges in engineering effective, cell-based composites for repair of orthopaedic and cardiovascular tissues. Whether repairing articular cartilage, bone, or blood vessel, the demands placed on the surgical implants can threaten the long-term success of the procedure. In 1998, the US National Committee on Biomechanics addressed this problem by suggesting a new paradigm for tissue engineering called “functional tissue engineering” or FTE. FTE seeks to address several important questions. What are the biomechanical demands placed upon the normal tissue and hence the tissue engineered implant after surgery? What parameters should a tissue engineer design into the implant before surgery? And what biomechanical parameters should the tissue engineer track to determine if the resulting repair is successful? To illustrate the principles, this presentation will discuss tendon repair as a model system for functional tissue engineering.

scholarly journals The Influence of Exposure Energy Density on Porosity and Microhardness of the SLM Additive Manufactured Elements

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2304 ◽  
Author(s):  
Janusz Kluczyński ◽  
Lucjan Śnieżek ◽  
Krzysztof Grzelak ◽  
Janusz Mierzyński
Keyword(s):  
Computer Aided Design ◽  
Complex Geometry ◽  
Final Analysis ◽  
Machine Building ◽  
Layer By Layer ◽  
Laser Melting ◽  
Measurement Results ◽  
Computer Aided ◽  
Exposure Energy ◽  
Aided Design

Selective laser melting (SLM) is an additive manufacturing technique. It allows elements with very complex geometry to be produced using metallic powders. A geometry of manufacturing elements is based only on 3D computer-aided design (CAD) data. The metal powder is melted selectively layer by layer using an ytterbium laser. This paper contains the results of porosity and microhardness analysis made on specimens manufactured during a specially prepared process. Final analysis helped to discover connections between changing hatching distance, exposure speed and porosity. There were no significant differences in microhardness and porosity measurement results in the planes perpendicular and parallel to the machine building platform surface.

Current trends in the design of scaffolds for computer-aided tissue engineering

2014 ◽  
Vol 10 (2) ◽  
pp. 580-594 ◽  
Author(s):  
S.M. Giannitelli ◽  
D. Accoto ◽  
M. Trombetta ◽  
A. Rainer
Keyword(s):  
Tissue Engineering ◽  
Current Trends ◽  
Computer Aided

Effect of process parameters on the properties of selective laser sintered Poly(3-hydroxybutyrate) scaffolds for bone tissue engineering

2012 ◽  
Vol 7 (4) ◽  
pp. 275-285 ◽  
Author(s):  
T.F. Pereira ◽  
M.A.C. Silva ◽  
M.F. Oliveira ◽  
I.A. Maia ◽  
J.V.L. Silva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Process Parameters

Vascularization strategies for tissue engineering for tracheal reconstruction

2021 ◽  
Author(s):  
Fei Sun ◽  
Yi Lu ◽  
Zhihao Wang ◽  
Hongcan Shi
Keyword(s):  
Tissue Engineering ◽  
Cell Metabolism ◽  
Microvascular Network ◽  
Related Factors ◽  
Engineering Technology ◽  
Term Survival ◽  
Tracheal Reconstruction ◽  
Tracheal Replacement ◽  
Therapy Strategies

Tissue engineering technology provides effective alternative treatments for tracheal reconstruction. The formation of a functional microvascular network is essential to support cell metabolism and ensure the long-term survival of grafts. Although several tracheal replacement therapy strategies have been developed in the past, the critical significance of the formation of microvascular networks in 3D scaffolds has not attracted sufficient attention. Here, we review key technologies and related factors of microvascular network construction in tissue-engineered trachea and explore optimized preparation processes of vascularized functional tissues for clinical applications.

Process Characterization and the Effect of Process Defects on Flip Chip Reliability

1999 ◽  
Author(s):  
Brian J. Lewis ◽  
Hilary Sasso
Keyword(s):  
Process Parameters ◽  
Flip Chip ◽  
Failure Modes ◽  
Shock Testing ◽  
Fine Pitch ◽  
Process Characterization ◽  
Solder Bumps ◽  
Optimum Height ◽  
High Yields

Abstract Processing fine pitch flip chip devices continues to pose problems for packaging and manufacturing engineers. Optimizing process parameters such that defects are limited and long-term reliability of the assembly is increased can be a very tedious task. Parameters that effect the robustness of the process include the flux type and placement parameters. Ultimately, these process parameters can effect the long-term reliability of the flip chip assembly by either inhibiting or inducing process defects. Therefore, care is taken to develop a process that is robust enough to supply high yields and long term reliability, but still remains compatible with a standard surface mount technology process. This is where process optimization becomes most critical and difficult. What is the optimum height of the flux thin film used for a dip process? What force is required to insure that the solder bumps make contact with the pads? What are the limiting boundaries in which high yields and high reliabilities are achieved, while maintaining a streamlined, proven process? The following study evaluates a set of process parameters and their impact on process defects and reliability. The study evaluates process parameters including, flux type, flux application parameters, placement force and placement accuracy to determine their impact. Solder voiding, inadequate solder wetting, and crack propagation and delamination in the underfill layer are defects examined in the study. Assemblies will be subjected to liquid-to-liquid thermal shock testing (−55° C to 125°C) to determine failure modes due to the aforementioned defects. The results will show how changes in process parameters effect yield and reliability.

A multi-scale E-Jet 3D printing regulated by structured multi-physics field

2021 ◽  
Author(s):  
Kai Li ◽  
Yihui Zhao ◽  
Maiqi Liu ◽  
Xiaoying Wang ◽  
Fangyuan Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Aspect Ratio ◽  
Process Parameters ◽  
High Aspect Ratio ◽  
Thermal Field ◽  
Structural Features ◽  
Microfluidic Chips ◽  
Multi Scale ◽  
Fluid Field

Abstract Micro/nano scale structure as important functional part have been widely used in wearable flexible sensors, gas sensors, biological tissue engineering, microfluidic chips super capacitors and so on. Here a multi-scale electrohydrodynamic jet (E-Jet) 3D printing approach regulated by structured multi-physics fields was demonstrated to generate 800 nm scale 2D geometries and high aspect ratio 3D structures. The simulation model of jetting process under resultant effect of top fluid field, middle electric field and bottom thermal field was established. And the physical mechanism and scale law of jet formation were studied. The effects of thermal field temperature, applied voltage and flow rate on the jet behaviors were studied; and the range of process parameters of stable jet was obtained. The regulation of printing parameters was used to manufacture the high resolution gradient graphics and the high aspect ratio structure with tight interlayer bonding. The structural features could be flexibly adjusted by reasonably matching the process parameters. Finally, PCL/PVP composite scaffolds with cell-scale fiber and ordered fiber spacing were printed. The proposed E-Jet printing method provides an alternative approach for the application of biopolymer materials in tissue engineering.

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