Preparation, characterisation and in-vitro biocompatibility study of a bone graft developed from waste bovine teeth for bone regeneration

2020 ◽  
Vol 22 ◽  
pp. 100732 ◽  
Author(s):  
Jithendra T. Ratnayake ◽  
Eden D. Ross ◽  
George J. Dias ◽  
Kyle M. Shanafelt ◽  
Shay S. Taylor ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
In Vitro Biocompatibility ◽  
Biocompatibility Study

scholarly journals PDMS-enhanced slowly degradable Ca-P-Si scaffold: Material characterization, fabrication and in vitro biocompatibility study

2021 ◽  
Vol 19 ◽  
pp. 228080002110232
Author(s):  
Tao Wu ◽  
Zhanpeng Li ◽  
Yadong Chen ◽  
Qiang Liu ◽  
Jingshu Zhang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Water Distribution ◽  
Bmp Signaling ◽  
Apatite Formation ◽  
Osteoblast Cells ◽  
In Vitro Biocompatibility ◽  
Phosphate Silicate ◽  
Biocompatibility Study

A slowly degradable bone scaffold can well maintain the balance between new bone regeneration and scaffold resorption, esp. for seniors or patients suffering from pathological diseases, because too fast degradation can lead to the loss of long-term biological stability and result in scaffold failure. In this present study, calcium phosphate silicate (CPS) and polydimethylsiloxane (PDMS) were blended in different ratios to formulate slurries for scaffold fabrication. The effects of crosslinked PDMS on the CPS material properties were first characterized and the most viable formulation of CPS-PDMS slurry was determined based on the aforementioned results to 3D fabricate scaffolds. The biocompatibility of CPS-PDMS was further evaluated based on the scaffold extract’s cytotoxicity to osteoblast cells. Furthermore, real-time PCR was used to investigate the effects of scaffold extract to increase osteoblast proliferation. It is showed that the crosslinked PDMS interfered with CPS hydration and reduced both setting rate and compressive strength of CPS. In addition, CPS porosity was also found to increase with PDMS due to uneven water distribution as a result of increased hydrophobicity. Degradation and mineralization studies show that CPS-PDMS scaffold was slowly degradable and induced apatite formation. In addition, the in vitro analyses show that the CPS-PDMS scaffold did not exert any cytotoxic effect on osteoblast cells but could improve the cell proliferation via the TGFβ/BMP signaling pathway. In conclusion, CPS-PDMS scaffold is proved to be slowly degradable and biocompatible. Further analyses are therefore needed to demonstrate CPS-PDMS scaffold applications in bone regeneration.

scholarly journals A GREEN APPROACH FOR THE SYNTHESIS OF DRUG DELIVERY SYSTEM, MESOPOROUS SILICA GRAFTED ACRYLAMIDE –β- CYCLODEXTRIN COMPOSITE, FOR THE CONTROLLED RELEASE OF CURCUMIN

2018 ◽  
Vol 11 (9) ◽  
pp. 372
Author(s):  
Manohar D Mullassery ◽  
Noeline B Fernandez ◽  
Surya R ◽  
Diana Thomas
Keyword(s):  
Drug Delivery ◽  
Controlled Delivery ◽  
X Ray Diffraction ◽  
In Vitro Biocompatibility ◽  
Green Approach ◽  
Solvent Free Conditions ◽  
Good Biocompatibility ◽  
Biocompatibility Study

Objective: The scope of the present study was the preparation and characterization of a novel composite acrylamide β-cyclodextrin grafted 3-aminopropyltriethoxysilane bentonite (AMCD-g-APSB), for the controlled delivery of curcumin (CUR).Methods: AMCD-g-APSB, was synthesized by solvent-free conditions using microwave irradiation. The structure and surface morphology of the composite was established using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, etc.Results: The swelling percentage of the composite depends on both time and pH of the medium. The maximum swelling of the composite occurred at a pH of 7.4. The maximum drug encapsulation was occurring at a pH 3. About 96.5% of drug was loaded at pH 3. In vitro biocompatibility study was performed, and the result showed good biocompatibility of the composite in the concentration range 2.5–50 μg/ml.Conclusions: Drug delivery study of the composite proved that CUR could be successfully released in a controlled manner in the colon without much loses of the drug in the stomach.

scholarly journals Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 497 ◽  
Author(s):  
Moumita Ghosh ◽  
Michal Halperin-Sternfeld ◽  
Itzhak Grinberg ◽  
Lihi Adler-Abramovich
Keyword(s):  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Bone Tissue Regeneration ◽  
Pcr Analysis ◽  
Alizarin Red ◽  
In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

Fabrication and in-vitro biocompatibility of freeze-dried CTS-nHA and CTS-nBG scaffolds for bone regeneration applications

2020 ◽  
Vol 149 ◽  
pp. 1-10 ◽  
Author(s):  
Pawan Kumar ◽  
Meenu Saini ◽  
Brijnandan S. Dehiya ◽  
Ahmad Umar ◽  
Anil Sindhu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
In Vitro Biocompatibility ◽  
Freeze Dried

In vitro and in vivo biocompatibility study on acellular sheep periosteum for guided bone regeneration

2020 ◽  
Vol 15 (1) ◽  
pp. 015013
Author(s):  
Jing He ◽  
Zhenning Li ◽  
Tianhao Yu ◽  
Weizuo Wang ◽  
Meihan Tao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Biocompatibility Study
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