Combinatorial delivery of superparamagnetic iron oxide nanoparticles (γFe 2 O 3 ) and doxorubicin using folate conjugated redox sensitive multiblock polymeric nanocarriers for enhancing the chemotherapeutic efficacy in cancer cells

2017 ◽  
Vol 75 ◽  
pp. 1128-1143 ◽  
Author(s):  
Chetan Nehate ◽  
M.R. Aji Alex ◽  
Arun Kumar ◽  
Veena Koul
Keyword(s):  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Polymeric Nanocarriers ◽  
Chemotherapeutic Efficacy

scholarly journals Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

2021 ◽  
Author(s):  
Ali Ghorbani Ranjbary ◽  
Golnaz Karbalaei Saleh ◽  
Mohammadreza Azimi
Keyword(s):  
Dna Damage ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Mtt Assay ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Ht 29

Abstract Today, research into nanoparticles for diagnostic and therapeutic use in cancer is on the rise. In the present study, the effect of superparamagnetic iron oxide nanoparticles on the formation of apoptosis in HT-29 cells is investigated.In this study, we investigated the mechanisms of apoptosis induced by superparamagnetic iron oxide nanoparticles after MTT assay and determining the appropriate dose of 2.5 µg / mL to induce apoptosis in HT-29 cells.Superparamagnetic iron oxide nanoparticles increased the levels of ROS, Ca2 +, and DNA damage in HT-29 cells. Also, at the level of protein and mRNA, they increased the expression of Caspes 3 and 9 and significantly decreased Bcl-2 compared to the control group (P <0.0001).Fe3O4 causes apoptosis in cancer cells by increasing the level of ROS and intracellular calcium, followed by increasing the expression of caspases 3 and 9 and decreasing the expression of Bcl-2, as well as direct DNA damage.

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