Design and synthesis of reduced graphene oxide based supramolecular scaffold: A benign microbial resistant network for enzyme immobilization and cell growth

2017 ◽  
Vol 75 ◽  
pp. 1168-1177 ◽  
Author(s):  
Maloy Kr. Mondal ◽  
Suprabhat Mukherjee ◽  
Swadhin K. Saha ◽  
Pranesh Chowdhury ◽  
Santi P. Sinha Babu
2019 ◽  
Vol 9 (2) ◽  
pp. 326 ◽  
Author(s):  
Somasekhar Chinnadayyala ◽  
Jinsoo Park ◽  
Yonghyun Choi ◽  
Jae-Hee Han ◽  
Ajay Yagati ◽  
...  

The improved binding ability of graphene–nanoparticle composites to proteins or molecules can be utilized to develop new cell-based assays. In this study, we fabricated reduced graphene oxide–gold nanoparticles (rGO-AuNP) electrodeposited onto a transparent indium tin oxide (ITO) electrode and investigated the feasibility of the electrochemical impedance monitoring of cell growth. The electrodeposition of rGO–AuNP on the ITO was optically and electrochemically characterized in comparison to bare, rGO-, and AuNP-deposited electrodes. The cell growth on the rGO–AuNP/ITO electrode was analyzed via electrochemical impedance measurement together with the microscopic observation of HEK293 cells transfected with a green fluorescent protein expression vector. The results showed that rGO–AuNP was biocompatible and induced an increase in cell adherence to the electrode when compared to the bare, AuNP-, or rGO-deposited ITO electrode. At 54 h cultivation, the average and standard deviation of the saturated normalized impedance magnitude of the rGO–AuNP/ITO electrode was 3.44 ± 0.16, while the value of the bare, AuNP-, and rGO-deposited ITO electrode was 2.48 ± 0.15, 2.61 ± 0.18, and 3.01 ± 0.25, respectively. The higher saturated value of the cell impedance indicates that the impedimetric cell-based assay has a broader measurement range. Thus, the rGO–AuNP/ITO electrode can be utilized for label-free and real-time impedimetric cell-based assays with wider dynamic range.


2017 ◽  
Vol 9 (3) ◽  
pp. 2213-2222 ◽  
Author(s):  
Sanjay K. S. Patel ◽  
Seung Ho Choi ◽  
Yun Chan Kang ◽  
Jung-Kul Lee

2018 ◽  
Vol 6 (11) ◽  
pp. 2987-2997 ◽  
Author(s):  
M. C. Serrano ◽  
M. J. Feito ◽  
A. González-Mayorga ◽  
R. Diez-Orejas ◽  
M. C. Matesanz ◽  
...  

rGO microfibers mediate polarization of RAW-264.7 macrophages towards an M2 phenotype and support neural progenitor cell growth.


2019 ◽  
Vol 12 (5) ◽  
pp. 5775-5785 ◽  
Author(s):  
Yanxia Liu ◽  
Qiuchen Xu ◽  
Ran Wang ◽  
Yiteng Zheng ◽  
Zhen Wang ◽  
...  

2011 ◽  
Vol 21 (28) ◽  
pp. 10504 ◽  
Author(s):  
Huan-Wen Wang ◽  
Zhong-Ai Hu ◽  
Yan-Qin Chang ◽  
Yan-Li Chen ◽  
Hong-Ying Wu ◽  
...  

2021 ◽  
Vol 45 (15) ◽  
pp. 6630-6639
Author(s):  
Hanping Xu ◽  
Yue Li ◽  
Mengying Jia ◽  
Linlin Cui ◽  
Cheng Chen ◽  
...  

A novel, environmentally friendly and freestanding 3D flexible film electrode (CMC–PPy@RGO) was prepared by a simple vacuum filtration method.


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