A novel chitosan hydrogel membrane by an improved electrophoretic deposition and its characteristics in vitro and in vivo

2017 ◽  
Vol 74 ◽  
pp. 287-297 ◽  
Author(s):  
Wen-Wen Li ◽  
Hai-Yan Wang ◽  
Yu-Qing Zhang
Keyword(s):  
Electrophoretic Deposition ◽  
Chitosan Hydrogel ◽  
Hydrogel Membrane

scholarly journals Yb3+-containing chitosan hydrogels induce B-16 melanoma cell anoikis via a Fak-dependent pathway

2019 ◽  
Vol 8 (1) ◽  
pp. 645-660 ◽  
Author(s):  
Yu Miao ◽  
Jiawei Lu ◽  
Junhui Yin ◽  
Changchun Zhou ◽  
Yaping Guo ◽  
...  
Keyword(s):  
Acetic Acid Solution ◽  
Tumor Model ◽  
In Vivo Studies ◽  
High Recurrence Rate ◽  
Mixed Solution ◽  
Chitosan Hydrogel ◽  
Chitosan Hydrogels ◽  
Dermal Cells

AbstractMelanoma is the most lethal dermal tumor, and a high recurrence rate and skin defects are two main serious problems. An antimelanoma material,which effectively inhibits tumor recurrence and possesses excellent biocompatibility, is urgently needed to treat melanoma. In this study, we developed a novel antitumor Yb3+ [Yb(NO3)3]containing chitosan hydrogel (Yb-CS hydrogel) by dissolving Yb(NO3)3 and chitosan in acetic acid solution and forming composite hydrogels by a freeze-drying process after adding NaOH to the mixed solution. In vitro studies demonstrated that the Yb3+ produces effect of inducing cell death in Yb-CS hydrogel. Moreover, we found that the Yb-CS hydrogel inhibited a focal adhesion kinase (FAK)-dependent signaling pathway and induced B-16 cell anoikis. However, the Yb-CS hydrogel was less effective on L929 normal mouse dermal cells. In vivo studies showed that the Yb-CS hydrogel inhibited the recurrence of melanoma in a mouse bare xenograft tumor model. We concluded that the Yb-CS hydrogel could potentially be used in the antimelanoma field, especially in the inhibition of melanoma recurrence.

scholarly journals Retraction of “Flexible and Microporous Chitosan Hydrogel/Nano ZnO Composite Bandages for Wound Dressing: In Vitro and In Vivo Evaluation”

2019 ◽  
Vol 11 (31) ◽  
pp. 28596-28596 ◽  
Author(s):  
P. T. Sudheesh Kumar ◽  
Vinoth-Kumar Lakshmanan ◽  
T.V. Anilkumar ◽  
C. Ramya ◽  
P. Reshmi ◽  
...  
Keyword(s):  
Wound Dressing ◽  
In Vivo Evaluation ◽  
Chitosan Hydrogel ◽  
Nano Zno

Macrophage polarization in vitro and in vivo modified by contact with fragmented chitosan hydrogel

2021 ◽  
Author(s):  
Ysander Boxberg ◽  
Sylvia Soares ◽  
Camille Giraudon ◽  
Laurent David ◽  
Maud Viallon ◽  
...  
Keyword(s):  
Macrophage Polarization ◽  
Chitosan Hydrogel

Thermo-responsive chitosan hydrogel for healing of full-thickness wounds infected with XDR bacteria isolated from burn patients: In vitro and in vivo animal model

2020 ◽  
Vol 164 ◽  
pp. 4475-4486
Author(s):  
Zahra Aliakbar Ahovan ◽  
Sadjad Khosravimelal ◽  
Behnaz Sadat Eftekhari ◽  
Soraya Mehrabi ◽  
Ali Hashemi ◽  
...  
Keyword(s):  
Animal Model ◽  
Burn Patients ◽  
Full Thickness ◽  
Chitosan Hydrogel ◽  
In Vivo Animal Model

scholarly journals 259 Injectable chitosan hydrogel for localized delivery of immune checkpoint inhibitors

2021 ◽  
Vol 9 (Suppl 3) ◽  
pp. A281-A281
Author(s):  
Siena Mantooth ◽  
David Zaharoff ◽  
Siena Mantooth
Keyword(s):  
Checkpoint Inhibitors ◽  
Release Kinetics ◽  
Laboratory Animals ◽  
Tumor Treatment ◽  
Chitosan Hydrogel ◽  
Tissue Phantom ◽  
Model Protein ◽  
Localized Delivery

BackgroundSystemic delivery of checkpoint inhibitors risks the development of immune-related adverse events (irAEs) in up to 85% of patients.1 Localized delivery methods with slow-release kinetics have the potential to avoid systemic exposure and reduce irAEs. Direct tumor injection is extremely difficult, as saline-based solutions are rapidly excluded from the high-pressure tumor environment. Utilizing hydrogels as a delivery medium and local depot can address this shortcoming. To this end, we developed an injectable chitosan-based hydrogel for intratumoral delivery of checkpoint antibodies.MethodsHydrogelLow-viscosity, 80% deacetylated chitosan (Heppe Medical Chitosan; Halle, Germany) was reacted with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the presence of β-glycerophosphate at room temperature for 48 hours. The mixture was then washed with ethanol and dried at 60°C. The resulting solid was dissolved in phosphate buffered saline (PBS) at concentrations from 35–70 mg/mL.In vitro release. 300 μg/mL bovine serum albumin (BSA) labeled with fluorescein-5-isothiocyanate (FITC) as a model protein drug was loaded into the hydrogel. The hydrogel was injected through a 28g needle and incubated with PBS. Samples were taken over a week period. Release kinetics were analyzed by fitting fluorescence data to zero-order, first-order, and Korsmeyer-Peppas models. To visualize retention after injection, dye-loaded hydrogels or dye in PBS alone were injected into a 0.6 wt% agar tissue phantom.In vivo imaging and tumor treatment. Flank MC38 tumors will be established in C57BL/6 mice. At tumor volumes of 50–100 mm3, 200 ug of fluorescently labeled aCTLA-4 and aPD-L1 included in the chitosan hydrogel will be delivered intratumorally. Images will be captured using an In Vivo Imaging System (IVIS). Antitumor activity will be assessed in a separate cohort using unlabeled antibodies.ResultsThe chitosan hydrogel was found to be injectable in needles as thin as 28g. After exiting the needle, the hydrogel reformed (figure 1A). Upon injection into the tissue phantom, dyed PBS immediately leaked out, primarily through the needle track, while the dyed hydrogel was retained (figure 1B). In vitro release studies demonstrated long-term, nearly zero-order, week-long sustained release (figure 1C). In vivo retention and tumor treatment studies are ongoing.Abstract 259 Figure 1Injectable chitosan hydrogel. (A) Re-formed BSA-FITC hydrogel in 1x PBS; (B) (i) Retained hydrogel in agar tissue phantom, (ii) Excluded 1x PBS in agar tissue phantom; (C) Release kinetics in 1x PBS.ConclusionsA novel injectable chitosan hydrogel was found to provide sustained release of a large model protein over a 1–2 week period with favorable in vitro kinetics. Importantly, this hydrogel can be engineered to provide faster or slower release as needed. Ongoing studies in vivo will quantify intratumoral retention, systemic dissemination, and antitumor activity.AcknowledgementsThis work is supported by the National Science Foundation Graduate Research Fellowship.ReferenceHommes J, Verheijden R, Suijkerbuijk K, Hamann D. Biomarkers of checkpoint inhibitor induced immune-related adverse events—a comprehensive review. Front Oncol 2021;10:1–16.Ethics ApprovalAnimal use was in compliance with the Public Health Service Policy on Human Care and Use of Laboratory Animals. All experiments involving laboratory animals were approved by the Institutional Animal Care and Use Committee at North Carolina State University (Protocol #19–795).

Electrophoretic Deposition of Porous Ti Coatings for Bone Implants: In Vitro and In Vivo Evaluation

2015 ◽  
Vol 654 ◽  
pp. 144-148
Author(s):  
Annabel Braem ◽  
Bram Neirinck ◽  
Omer Van der Biest ◽  
Jef Vleugels
Keyword(s):  
Electrophoretic Deposition ◽  
Bone Ingrowth ◽  
Processing Route ◽  
Porous Ti ◽  
High Adhesion ◽  
Metallurgical Processing ◽  
Plasma Sprayed ◽  
Potential Use

A new powder metallurgical processing route for porous Ti coatings on Ti-6Al-4V substrates based on the electrophoretic deposition (EPD) of TiH2 suspensions is presented. After dehydrogenation and sintering in vacuum, coatings with a fully interconnected porosity (up to 51%, interconnective pore channels (IPC) of 2-50 µm) and high adhesion strength (up to 47 MPa) are obtained. Further evaluation of these coatings for potential use in biomedical implants shows that EPD Ti coatings are significantly less prone to bacterial adhesion compared to state-of-the-art vacuum plasma sprayed (VPS) coatings, while still allowing substantial bone ingrowth. Using EPD, the coating process can easily be transferred to complex-shaped implant components.

scholarly journals Bi-functional oxidized dextran–based hydrogel inducing microtumors: An in vitro three-dimensional lung tumor model for drug toxicity assays

2017 ◽  
Vol 8 ◽  
pp. 204173141771839 ◽  
Author(s):  
Dhaval Kedaria ◽  
Rajesh Vasita
Keyword(s):  
Drug Screening ◽  
Drug Toxicity ◽  
Disease Modeling ◽  
Lung Tumor ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Chitosan Hydrogel ◽  
Oxidized Dextran

Cancer is a serious death causing disease having 8.2 million deaths in 2012. In the last decade, only about 10% of chemotherapeutic compounds showed productivity in drug screening. Two-dimensional culture assays are the most common in vitro drug screening models, which do not precisely model the in vivo condition for reliable preclinical drug screening. Three-dimensional scaffold–based cell cultures perhaps mimic tumor microenvironment and recapitulate physiologically more relevant tumor. This study was carried out to develop bi-functional oxidized dextran–based cell instructive hydrogel that provides three-dimensional environment to cancer cells for inducing microtumor. Oxidized dextran was blended with thiolated chitosan to fabricate an in situ self-gelable hydrogel (modified dextran–chitosan) in a one-step process. The hydrogels characterization revealed cross-linked network structure with highly porous structure and water absorption. The modified dextran–chitosan hydrogel showed reduced hydrophobicity and has reduced protein absorption, which resulted in changing the A549 cell adhesiveness, and encouraged them to form microtumor. The cells were proliferated in clusters having spherical morphology with randomly oriented stress fiber and large nucleus. Further microtumors were studied for hypoxia where reactive oxygen species generation demonstrated 15-fold increase as compared to monolayer culture. Drug-sensitivity results showed that microtumors generated on modified dextran–chitosan hydrogel showed resistance to doxorubicin with having 33%–58% increased growth than two-dimensional monolayer model at concentrations of 25–100 µM. In summary, the modified dextran–chitosan scaffold can provide surface chemistry that induces three-dimensional microtumors with physiologically relevant properties to in vivo tumor including growth, morphology, extracellular matrix production, hypoxic phenotype, and drug response. This model can be potentially utilized for drug toxicity studies and cancer disease modeling to understand tumor phenotype and progression.

BMSCs laden injectable amino-diethoxypropane modified alginate-chitosan hydrogel for hyaline cartilage reconstruction

2015 ◽  
Vol 3 (9) ◽  
pp. 1990-2005 ◽  
Author(s):  
Wenbing Wan ◽  
Qingtao Li ◽  
Haiyun Gao ◽  
Liangpeng Ge ◽  
Yuqing Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Hyaline Cartilage ◽  
Chitosan Hydrogel ◽  
Injectable Hydrogel ◽  
Cartilage Reconstruction

We developed an injectable hydrogel composed of amino-diethoxypropane modified alginate and chitosan, and also investigated bone marrow mesenchy + mal stromal cells (BMSCs) laden hydrogel for cartilage reconstruction in vitro and in vivo.

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