Nanostructured poly (lactic acid) electrospun fiber with high loadings of TiO2 nanoparticles: Insights into bactericidal activity and cell viability

2017 ◽  
Vol 71 ◽  
pp. 381-385 ◽  
Author(s):  
T.V. Toniatto ◽  
B.V.M. Rodrigues ◽  
T.C.O. Marsi ◽  
R. Ricci ◽  
F.R. Marciano ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Viability ◽  
Tio2 Nanoparticles ◽  
Bactericidal Activity ◽  
Electrospun Fiber ◽  
Poly Lactic Acid

scholarly journals Drug Release from Electrospun Poly(Lactic Acid) Membranes and Their Cell Viability in Vitro Test

2012 ◽  
Vol 44 ◽  
pp. 866-868 ◽  
Author(s):  
A.P.S. Immich ◽  
M. Lis ◽  
L.H. Catalani ◽  
R.L. Boemo ◽  
J.A. Tornero
Keyword(s):  
Lactic Acid ◽  
Drug Release ◽  
Cell Viability ◽  
Poly Lactic Acid ◽  
In Vitro Test ◽  
Vitro Test

A superhydrophobic poly(lactic acid) electrospun nanofibrous membrane surface-functionalized with TiO2 nanoparticles and methyltrichlorosilane for oil/water separation and dye adsorption

2019 ◽  
Vol 43 (39) ◽  
pp. 15823-15831 ◽  
Author(s):  
Zixuan Zhou ◽  
Lejing Liu ◽  
Weizhong Yuan
Keyword(s):  
Methylene Blue ◽  
Lactic Acid ◽  
Tio2 Nanoparticles ◽  
Membrane Surface ◽  
Dye Adsorption ◽  
Nanofibrous Membrane ◽  
Poly Lactic Acid ◽  
Water Separation ◽  
Oil Water Separation ◽  
Oil Water

A superhydrophobic PLA electrospun nanofibrous membrane surface-functionalized with TiO2 nanoparticles and methyltrichlorosilane can achieve oil/water separation and methylene blue adsorption.

Preparation and characterization of poly(lactic acid)-grafted TiO2 nanoparticles with improved dispersions

2009 ◽  
Vol 255 (15) ◽  
pp. 6795-6801 ◽  
Author(s):  
Yan-Bing Luo ◽  
Xiu-Li Wang ◽  
Da-Yun Xu ◽  
Yu-Zhong Wang
Keyword(s):  
Lactic Acid ◽  
Tio2 Nanoparticles ◽  
Poly Lactic Acid

scholarly journals Poly (Lactic Acid) Electrospun fibers added with Rumex hymenosepalus extract: preparation and characterization.

Biotecnia ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 108-115
Author(s):  
Lorena Armenta Villegas
Keyword(s):  
Lactic Acid ◽  
Electrospun Fiber ◽  
Electrospun Nanofibers ◽  
Alkaline Treatment ◽  
Wound Dressings ◽  
Poly Lactic Acid ◽  
Electrospun Fibers ◽  
Interaction Sites ◽  
Vis Spectroscopy ◽  
Parameter Modification

In this work Poly (lactic acid) (PLA) electrospun fibers added with Rumex hymenosepalus extract were prepared and characterized. A morphologic characterization study gave support to choose the parameters that allow the obtention of uniform fibers, within the parameters that was studied were rate flow varied from 0.8-2.0 mL/h and voltage variation between 15-20 kV. The morphology of the electrospun nanofibers was examined by scanning electron microcopy, statistical study was made to identify the fibers diameters obtained with parameter modification. Since PLA is well known as hydrophobic material, an alkaline hydrolysis was performed to improve the interaction sites of the electrospun fiber and R. hymenosepalus extract. FTIR-ATR study was performed to evaluate the behavior of the PLA electrospun fibers and PLA hydrolyzed electrospun fibers and it´s interaction with the R. hymenosepalus extract. Antioxidant release study was carried out using UV-Vis spectroscopy and with this evaluation was determined that the alkaline treatment help to control the Rh release rate. The obtained results let the electrospun fibers of PLA-Rh were considered useful for antioxidant release applications like wound dressings.

Effects of TiO2 nanoparticles on the photodegradation of poly(lactic acid)

2018 ◽  
Vol 135 (30) ◽  
pp. 46509 ◽  
Author(s):  
Yanbing Luo ◽  
Yuzhen Cao ◽  
Gang Guo
Keyword(s):  
Lactic Acid ◽  
Tio2 Nanoparticles ◽  
Poly Lactic Acid

scholarly journals Volume-by-volume bioprinting of chondrocytes-alginate bioinks in high temperature thermoplastic scaffolds for cartilage regeneration

2019 ◽  
Vol 244 (1) ◽  
pp. 13-21 ◽  
Author(s):  
JM Baena ◽  
G Jiménez ◽  
E López-Ruiz ◽  
C Antich ◽  
C Griñán-Lisón ◽  
...  
Keyword(s):  
Lactic Acid ◽  
High Temperature ◽  
Cell Viability ◽  
High Temperatures ◽  
Cartilage Tissue ◽  
Poly Lactic Acid ◽  
Layer By Layer ◽  
3D Bioprinting ◽  
Thermoplastic Polymers ◽  
Mesh Structure

Biofabrication technologies with layer-by-layer simultaneous deposition of a polymeric matrix and cell-laden bioinks (also known as bioprinting) offer an alternative to conventional treatments to regenerate cartilage tissue. Thermoplastic polymers, like poly-lactic acid, are easy to print using fused deposition modeling, and the shape, mesh structure, biodegradation time, and stiffness can be easily controlled. Besides some of them being clinically approved, the high manufacturing temperatures used in bioprinting applications with these clinically available thermoplastics decrease cell viability. Geometric restriction prevents cell contact with the heated printed fibers, increasing cell viability but comprising the mechanical performance and biodegradation time of the printed parts. The objective of this study was to develop a novel volume-by-volume 3D-biofabrication process that divides the printed part into different volumes and injects the cells after each volume has been printed, once the temperature of the printed thermoplastic fibers has decreased. In order to show the suitability of this process, chondrocytes were isolated from osteoarthritic patient samples and after characterization were used to test the feasibility of the process. Human chondrocytes were bioprinted together with poly-lactic acid and apoptosis, proliferation and metabolic activity were analyzed. This novel volume-by-volume 3D-biofabrication procedure prints a mesh structure layer-by-layer with a high adhesion surface/volume ratio, driving a rapid decrease in the temperature, avoiding contact with cells in high temperature zones. In our study, chondrocytes survived the manufacturing process, with 90% of viability, 2 h after printing, and, after seven days in culture, chondrocytes proliferated and totally colonized the scaffold. The use of the volume-by-volume-based biofabrication process presented in this study shows valuable potential in the short-term development of bioprint-based clinical therapies for cartilage injuries. Impact statement 3D bioprinting represents a novel advance in the area of regenerative biomedicine and tissue engineering for the treatment of different pathologies, among which are those related to cartilage. Currently, the use of different thermoplastic polymers, such as PLA or PCL, for bioprinting processes presents an important limitation: the high temperatures that are required for extrusion affect the cell viability and the final characteristics of the construct. In this work, we present a novel bioprinting process called volume-by-volume (VbV) that allows us to preserve cell viability after bioprinting. This procedure allows cell injection at a safe thermoplastic temperature, and also allows the cells to be deposited in the desired areas of the construct, without the limitations caused by high temperatures. The VbV process could make it easier to bring 3D bioprinting into the clinic, allowing the generation of tissue constructs with polymers that are currently approved for clinical use.

scholarly journals In Vitro Degradation of Plasticized PLA Electrospun Fiber Mats: Morphological, Thermal and Crystalline Evolution

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2975
Author(s):  
Adrián Leonés ◽  
Laura Peponi ◽  
Marcela Lieblich ◽  
Rosario Benavente ◽  
Stefano Fiori
Keyword(s):  
Lactic Acid ◽  
Electrospun Fiber ◽  
Hydrolytic Degradation ◽  
Degree Of Crystallinity ◽  
Poly Lactic Acid ◽  
In Vitro Test ◽  
Fiber Mats ◽  
Whole Process ◽  
Vitro Test

In the present work, fiber mats of poly(lactic acid), PLA, plasticized by different amounts of oligomer lactic acid, OLA, were obtained by electrospinning in order to investigate their long term hydrolytic degradation. This was performed in a simulated body fluid for up to 352 days, until the complete degradation of the samples is reached. The evolution of the plasticized electrospun mats was followed in terms of morphological, thermal, chemical and crystalline changes. Mass variation and water uptake of PLA-based electrospun mats, together with pH stability of the immersion media, were also studied during the in vitro test. The results showed that the addition of OLA increases the hydrolytic degradation rate of PLA electrospun fiber mats. Moreover, by adding different amounts of OLA, the time of degradation of the electrospun fiber mats can be modulated over the course of a year. Effectively, by increasing the amount of OLA, the diameter of the electrospun fibers decreases more rapidly during degradation. On the other hand, the degree of crystallinity and the dimension of the α crystals of the electrospun fiber mats are highly affected not only by the presence but also by the amount of OLA during the whole process.

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