Potentiostatic pulse-deposition of calcium phosphate on magnesium alloy for temporary implant applications — An in vitro corrosion study

2013 ◽  
Vol 33 (2) ◽  
pp. 675-679 ◽  
Author(s):  
M. Bobby Kannan ◽  
O. Wallipa
Keyword(s):  
Magnesium Alloy ◽  
Calcium Phosphate ◽  
Corrosion Study ◽  
Pulse Deposition

In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy

Biomaterials ◽  
2009 ◽  
Vol 30 (8) ◽  
pp. 1512-1523 ◽  
Author(s):  
Liping Xu ◽  
Feng Pan ◽  
Guoning Yu ◽  
Lei Yang ◽  
Erlin Zhang ◽  
...  
Keyword(s):  
Magnesium Alloy ◽  
Calcium Phosphate ◽  
In Vivo Evaluation

scholarly journals In Vitro Corrosion Study of Friction Stir Processed WE43 Magnesium Alloy in a Simulated Body Fluid

Materials ◽  
2016 ◽  
Vol 9 (7) ◽  
pp. 542 ◽  
Author(s):  
Genghua Cao ◽  
Datong Zhang ◽  
Weiwen Zhang ◽  
Wen Zhang
Keyword(s):  
Magnesium Alloy ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Corrosion Study ◽  
Friction Stir ◽  
We43 Magnesium Alloy

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials

2006 ◽  
Vol 69 (12) ◽  
pp. 976-982 ◽  
Author(s):  
Nenad Ignjatović ◽  
Petar Ninkov ◽  
Vesna Kojić ◽  
Miloš Bokurov ◽  
Vladimir Srdić ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals Calcium phosphate phase transition in the presence of Aminosilane

2014 ◽  
Vol 70 (a1) ◽  
pp. C67-C67
Author(s):  
Babak Mostaghaci ◽  
Brigitta Loretz ◽  
Robert Haberkorn ◽  
Guido Kickelbick ◽  
Claus-Michael Lehr
Keyword(s):  
Phase Transition ◽  
Calcium Phosphate ◽  
Blood Compatibility ◽  
Transfection Efficiency ◽  
Hek293 Cells ◽  
Step Method ◽  
Calcium Phosphate Nanoparticles ◽  
Amine Groups ◽  
The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

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