Probing lateral diffusion in lipid membranes on nanoscale by PFG NMR with high gradient strength

2007 ◽  
Vol 25 (4) ◽  
pp. 493-496 ◽  
Author(s):  
Konstantin Ulrich ◽  
Monica Sanders ◽  
Sergey Vasenkov
Keyword(s):  
Lipid Membranes ◽  
Lateral Diffusion ◽  
High Gradient ◽  
Gradient Strength ◽  
Pfg Nmr

Application of Pulsed Field Gradient NMR with High Gradient Strength for Studies of Self-Diffusion in Lipid Membranes on the Nanoscale

Langmuir ◽  
2008 ◽  
Vol 24 (14) ◽  
pp. 7365-7370 ◽  
Author(s):  
Konstantin Ulrich ◽  
Monica Sanders ◽  
Farida Grinberg ◽  
Petrik Galvosas ◽  
Sergey Vasenkov
Keyword(s):  
Field Gradient ◽  
Lipid Membranes ◽  
Pulsed Field Gradient Nmr ◽  
High Gradient ◽  
Pulsed Field ◽  
Self Diffusion ◽  
Gradient Strength

scholarly journals Induction of Highly Curved Structures in Relation to Membrane Permeabilization and Budding by the Triterpenoid Saponins, α- and δ-Hederin

2013 ◽  
Vol 288 (20) ◽  
pp. 14000-14017 ◽  
Author(s):  
Joseph Lorent ◽  
Cécile S. Le Duff ◽  
Joelle Quetin-Leclercq ◽  
Marie-Paule Mingeot-Leclercq
Keyword(s):  
Lipid Membranes ◽  
Pore Formation ◽  
Lateral Diffusion ◽  
Molecular Shape ◽  
Membrane Curvature ◽  
Membrane Permeabilization ◽  
Spontaneous Curvature ◽  
Unilamellar Vesicles ◽  
Generalized Polarization ◽  
Triterpenoid Saponins

The interactions of triterpenoid monodesmosidic saponins, α-hederin and δ-hederin, with lipid membranes are involved in their permeabilizing effect. Unfortunately, the interactions of these saponins with lipid membranes are largely unknown, as are the roles of cholesterol or the branched sugar moieties (two for α-hederin and one for δ-hederin) on the aglycone backbone, hederagenin. The differences in sugar moieties are responsible for differences in the molecular shape of the saponins and the effects on membrane curvature that should be the most positive for α-hederin in a transbilayer direction. In large unilamellar vesicles and monocyte cells, we showed that membrane permeabilization was dependent on the presence of membrane cholesterol and saponin sugar chains, being largest for α-hederin and smallest for hederagenin. In the presence of cholesterol, α-hederin induced the formation of nonbilayer phases with a higher rate of Brownian tumbling or lateral diffusion. A reduction of Laurdan's generalized polarization in relation to change in order of the polar heads of phospholipids was observed. Using giant unilamellar vesicles, we visualized the formation of wrinkled borders, the decrease in liposome size, budding, and the formation of macroscopic pores. All these processes are highly dependent on the sugars linked to the aglycone, with α-hederin showing a greater ability to induce pore formation and δ-hederin being more efficient in inducing budding. Hederagenin induced intravesicular budding but no pore formation. Based on these results, a curvature-driven permeabilization mechanism dependent on the interaction between saponin and sterols and on the molecular shape of the saponin and its ability to induce local spontaneous curvature is proposed.

Diffusion NMR and bicelle morphology

2011 ◽  
Vol 89 (9) ◽  
pp. 1021-1035 ◽  
Author(s):  
Peter M. Macdonald ◽  
Ronald Soong
Keyword(s):  
Lateral Diffusion ◽  
Diffusion Method ◽  
Nmr Studies ◽  
Diffusion Nmr ◽  
Magnetically Aligned Bicelles ◽  
Diffusion Studies ◽  
Membrane Bound ◽  
Nmr Diffusion ◽  
Pfg Nmr ◽  
Magnetically Aligned

This minireview focuses on diffusion NMR studies in bicelles. Following a discourse on diffusion fundamentals, and a comparative overview of fluorescence and NMR-based techniques for measuring diffusion, the pulsed field gradient (PFG) NMR diffusion method is introduced, emphasizing its specific advantages and limitations when applied to diffusion measurements in macroscopically oriented lamellar systems such as magnetically aligned bicelles. The utility of PFG NMR diffusion measurements in bicellar model membrane systems for examining lateral diffusion of membrane-bound molecular species is demonstrated, along with certain features of lateral diffusion that such studies illuminate. Further, those aspects of bicelle morphology that have been resolved using PFG NMR diffusion studies of various molecular weight soluble polymeric species are reviewed. The discussion concludes with an outline of future prospects for diffusion NMR studies in bicelles.

scholarly journals Bicellar Mixtures Containing Pluronic F68: Morphology and Lateral Diffusion from Combined SANS and PFG NMR Studies

Langmuir ◽  
2010 ◽  
Vol 26 (4) ◽  
pp. 2630-2638 ◽  
Author(s):  
Ronald Soong ◽  
Mu-Ping Nieh ◽  
Eric Nicholson ◽  
John Katsaras ◽  
Peter M. Macdonald
Keyword(s):  
Lateral Diffusion ◽  
Nmr Studies ◽  
Pluronic F68 ◽  
Pfg Nmr

Relation Between Structure and Diffusion in Nanostructured Porous Solids and in Lipid Membranes

2005 ◽  
Vol 899 ◽  
Author(s):  
Sergey Vasenkov ◽  
Jörg Kärger
Keyword(s):  
Lipid Membranes ◽  
Molecular Transport ◽  
Domain Size ◽  
Porous Solids ◽  
Diffusion Studies ◽  
Lipid Diffusion ◽  
Effective Diffusivities ◽  
Pfg Nmr ◽  
Fcc Catalysts ◽  
And Diffusion

AbstractPulsed field gradient (PFG) NMR technique has been applied to study molecular transport in two different types of nanostructured materials, viz. in fluid catalytic cracking (FCC) catalysts and in lipid membranes. Diffusion studies have been performed for a broad range of molecular displacements covering displacements that are as small as a fraction of a micron. The effective diffusivities recorded on various length scales are used to evaluate the relevance of various transport modes in the particles of FCC catalysts for the rate of molecular exchange between catalyst particles and the surrounding atmosphere. This rate is shown to be primarily related to the diffusion in the meso- and macropores of the particles under the condition of fast molecular exchange between these pores and the zeolite crystals located in the particles. Studies of lipid membranes are focused on developing fundamental understanding of the influence of various types of domains on lateral mobility of lipids. A meaningful study of this influence requires an ability of monitoring lipid diffusion for different displacements that are smaller and larger than the domain size. First PFG NMR data along this direction are presented.

Lipid lateral diffusion in binary and ternary systems of phosphatidylcholines, sphingomyelins and sterols studied by pfg-NMR

2007 ◽  
Vol 149 ◽  
pp. S13-S14
Author(s):  
Greger Orädd ◽  
Göran Lindblom ◽  
Katrin K. Halling ◽  
Thomas K.M. Nyholm ◽  
J. Peter Slotte
Keyword(s):  
Lateral Diffusion ◽  
Ternary Systems ◽  
Pfg Nmr ◽  
Binary And Ternary Systems

scholarly journals Enzyme Catalysis Enhances Lateral Lipid Motility and Directional Particle Transport on Membranes

2021 ◽  
Author(s):  
Ambika Somasundar ◽  
Niladri Sekhar Mandal ◽  
Ayusman Sen
Keyword(s):  
Lipid Membranes ◽  
Enzyme Catalysis ◽  
Lateral Diffusion ◽  
Cholesterol Content ◽  
Supported Bilayers ◽  
Membrane Fluctuations ◽  
Diffusion Enhancement ◽  
Membrane Bound ◽  
Passive Tracers ◽  
Membrane Bound Enzymes

The dynamic interplay between the composition of lipid membranes and the behavior of membrane-bound enzymes is critical to the understanding of cellular function and viability, and the design of membrane-based biosensing platforms. While there is a significant body of knowledge on how lipid composition and dynamics affect membrane-bound enzymes, little is known about how enzyme catalysis influences the motility and lateral transport in lipid membranes. Using enzymes-attached lipids in supported bilayers (SLB), we show catalysis-induced enhanced lateral diffusion of lipids in the bilayer. Enhancing the membrane viscosity by increasing the cholesterol content in the bilayer suppresses the overall diffusion but not the relative diffusion enhancement of the enzyme-attached lipids. We also provide direct evidence of catalysis-induced membrane fluctuations leading to the enhanced diffusion of passive tracers resting on the SLB. Additionally, by using active enzyme patches, we demonstrate the directional transport of tracers on SLBs. These are first steps in understanding diffusion and transport in lipid membranes due to active, out-of-equilibrium processes that are the hallmark of living systems. In general, our study demonstrates how active enzymes can be used to control diffusion and transport in confined 2-D environments.

scholarly journals Biophysical characterization reveals the similarities of liposomes produced using microfluidics and electroformation

2019 ◽  
Author(s):  
Michael Schaich ◽  
Diana Sobota ◽  
Hannah Sleath ◽  
Jehangir Cama ◽  
Ulrich F. Keyser
Keyword(s):  
Diffusion Coefficients ◽  
Lipid Membranes ◽  
Fluorescence Recovery After Photobleaching ◽  
Lateral Diffusion ◽  
Experimental Conditions ◽  
Biophysical Characterization ◽  
Lipid Mixtures ◽  
Significant Interest ◽  
Versatile Tool

AbstractGiant Unilamellar Vesicles (GUVs) are a versatile tool in many branches of science, including biophysics and synthetic biology. Octanol-Assisted Liposome Assembly (OLA), a recently developed microfluidic technique enables the production and testing of GUVs within a single device under highly controlled experimental conditions. It is therefore gaining significant interest as a platform for use in drug discovery, the production of artificial cells and more generally for controlled studies of the properties of lipid membranes. In this work, we expand the capabilities of the OLA technique by forming GUVs of tunable binary lipid mixtures of DOPC, DOPG and DOPE. Using fluorescence recovery after photobleaching we investigated the lateral diffusion coefficients of lipids in OLA liposomes and found the expected values in the range of 1 μm2/s for the lipid systems tested. We studied the OLA derived GUVs under a range of conditions and compared the results with electroformed vesicles. Overall, we found the lateral diffusion coefficients of lipids in vesicles obtained with OLA to be quantitatively similar to those in vesicles obtained via traditional electroformation. Our results provide a quantitative biophysical validation of the quality of OLA derived GUVs, which will facilitate the wider use of this versatile platform.

scholarly journals Lateral Diffusion Coefficients of an Eicosanyl-Based Bisglycerophosphocholine Determined by PFG-NMR and FRAP

Langmuir ◽  
2007 ◽  
Vol 23 (11) ◽  
pp. 6276-6280 ◽  
Author(s):  
Wilma Febo-Ayala ◽  
David P. Holland ◽  
Scott A. Bradley ◽  
David H. Thompson
Keyword(s):  
Diffusion Coefficients ◽  
Lateral Diffusion ◽  
Pfg Nmr

scholarly journals Pfg NMR studies of lateral diffusion in oriented lipid bilayers

2005 ◽  
Vol 19 (4) ◽  
pp. 191-198 ◽  
Author(s):  
Greger Orädd ◽  
Göran Lindblom
Keyword(s):  
Lipid Bilayers ◽  
Lateral Diffusion ◽  
Short Review ◽  
Lipid Domains ◽  
Nmr Studies ◽  
Membrane Biology ◽  
Diffusion Technique ◽  
Nmr Diffusion ◽  
Pfg Nmr

The pfg-NMR diffusion technique is proposed to have an appreciable potential for future biophysical investigations in the field of membrane biology. Topics like transport of molecules both across and in the plane of the membrane can be successfully studied, as well as the formation of lipid domains and their intrinsic dynamics can be scrutinized. This short review will introduce the fundamental aspects of orientation dependent NMR interactions and the technique of macroscopically oriented bilayers for eliminating the unwanted effects of those interactions. The pfg-NMR technique will be briefly introduced and finally, some recent results illustrating the potential of the method are presented.

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