Apoptosis in epididymis of sand rat Psammomys obesus, Cretzschmar, 1828: Effects of seasonal variations, castration and efferent duct ligation

Morphologie ◽  
2021 ◽  
Author(s):  
R. Menad ◽  
L. Lakabi ◽  
M. Fernini ◽  
S. Smaï ◽  
T. Gernigon Spychalowicz ◽  
...  
Keyword(s):  
Seasonal Variations ◽  
Psammomys Obesus ◽  
Sand Rat ◽  
Efferent Duct ◽  
Duct Ligation

GPER1 in sand rat epididymis: Effects of seasonal variations, castration and efferent ducts ligation

2017 ◽  
Vol 183 ◽  
pp. 9-20 ◽  
Author(s):  
Rafik Menad ◽  
Meriem Fernini ◽  
Souaâd Smaï ◽  
Xavier Bonnet ◽  
Thérèse Gernigon-Spychalowicz ◽  
...  
Keyword(s):  
Seasonal Variations ◽  
Sand Rat ◽  
Efferent Ducts ◽  
Rat Epididymis

scholarly journals Arrested apoptosis without nuclear fragmentation produced by efferent duct ligation in round spermatids and multinucleated giant cells of rat testis

Reproduction ◽  
2003 ◽  
pp. 879-887 ◽  
Author(s):  
E Anton
Keyword(s):  
Acid Phosphatase ◽  
Nuclear Membrane ◽  
Sertoli Cells ◽  
Giant Cells ◽  
Differential Staining ◽  
Multinucleated Giant Cells ◽  
Nuclear Fragmentation ◽  
Efferent Duct ◽  
Duct Ligation ◽  
Acid Phosphatase Reaction

The apoptotic process evoked by efferent duct ligation in the testes of adult rats was followed for 10 days by differential staining for haematoxylin-eosin, periodic acid-Schiff and a modified trichrome technique in optical microscopy and by ultrastructural localization of acid phosphatase. Round spermatids showed the first effects of efferent duct ligation. At day 3 after ligation, annular clumps of chromatin with typical apoptotic characteristics appeared against the nuclear membrane of these cells. Afterwards, membranous structures and a wide separation between the two layers of the nuclear membrane were observed but nuclear fragmentation did not occur and apoptotic granules were not seen. Cytoplasmic components were also altered, and severely damaged organoids and empty vacuoles lacking acid phosphatase reaction were frequently seen. On day 2 after efferent duct ligation, multinucleated giant cells appeared, which displayed similar characteristics as spermatids and showed no acid phosphatase reaction. Although abnormal spermatids and multinucleated giant cells were surrounded by the cytoplasm of Sertoli cells, neither lysosomal acid phosphatase nor phagocytic activity was detected. It is concluded that efferent duct ligation specifically affects round immature spermatids eliciting a partial nuclear apoptotic response that is not accompanied by autophagic or heterophagic activity and without lysosomal participation in Sertoli cells.

Studies on the "labile-bound" glucose compartment in erythrocytes: studies on Psammomys obesus (sand rat) and preliminary studies on human erythrocytes.

1985 ◽  
Vol 31 (7) ◽  
pp. 1219-1221 ◽  
Author(s):  
M Halperin ◽  
J H Adler
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Human Erythrocytes ◽  
Psammomys Obesus ◽  
Sand Rat ◽  
Fluid Compartment ◽  
Fast Kinetic ◽  
Fluid Compartments

Abstract Enzymatic (glucose oxidase) measurement of glucose concentration in the fluid compartment of Psammomys erythrocytes (Gfe) and of its concentration in the fluid compartment of blood plasma (Gfp) gives the ratio (mean +/- SD): Gfe/Gfp = 1.50 +/- 0.43 (n = 12, 23 degrees C). However, when we added 3H-labeled glucose (G*) in vitro to the whole blood, the ratio after 2 min was G*fe/G*fp = 0.90 (SD 0.11) and after 5 min G*fe/G*fp = 0.97 (SD 0.12). These calculations were based on previous determination of the fractional volumes of the fluid and non-fluid compartments in Psammomys blood. The results suggest that there is more than one compartment of measurable glucose in Psammomys erythrocytes. Glucose undergoes a fast free transfer between the plasma and the erythrocyte fluids, and a much slower transmission to another measurable compartment in the erythrocyte, where it is loosely bound to other molecules. This loosely bound glucose does not participate in the fast kinetic transmission across the erythrocyte membrane, but it is measurable by the glucose-oxidase-based method. Preliminary studies on human erythrocytes lead to similar conclusions.

Changes in testicular blood flow and testosterone production during aspermatogenesis after irradiation

1983 ◽  
Vol 98 (1) ◽  
pp. 35-46 ◽  
Author(s):  
J. Wang ◽  
K. A. A. Galil ◽  
B. P. Setchell
Keyword(s):  
Blood Flow ◽  
Leydig Cells ◽  
Capillary Permeability ◽  
Extracellular Fluid ◽  
Testis Weight ◽  
Γ Irradiation ◽  
Adult Rats ◽  
Efferent Duct ◽  
Duct Ligation ◽  
Testicular Blood Flow

Exposure of the testes of anaesthetized adult rats to 527 rads of γ-irradiation caused testis weight to fall slowly at first and then more rapidly from 21 days afterwards, reaching a minimum at 52 days, when spermatogenesis was severely disrupted. The weights of the accessory organs and the concentrations of testosterone in peripheral blood were slightly reduced; the concentrations in blood from the testicular veins were lower than control at shorter intervals after irradiation, but at later times tended to be similar or greater than control. Testicular blood flow per testis followed testis weight closely, and as a result the production of testosterone by the smaller testes (calculated as the product of plasma flow and the veno–arterial difference in testosterone concentration) was markedly reduced especially when the rats had been stimulated with human chorionic gonadotrophin (hCG). Serum FSH and LH rose appreciably as testis weight fell but there was a proportionately greater rise in FSH than LH, in comparison with surgically castrated animals. Increased amounts of extratubular, extracellular fluid were found in the aspermatogenic testes, but injection of hCG still caused increases in capillary permeability and the amount of fluid in the testis. These results indicate that during aspermatogenesis following irradiation (as with heat and efferent duct ligation) the capacity of the testes to secrete testosterone is severely limited by decreased testicular blood flow, not by the ability of the Leydig cells to release testosterone into their immediate environment.

scholarly journals Establishment of an albino sand rat (Psammomys obesus) colony and comparison with the natural coloured animal

1978 ◽  
Vol 12 (1) ◽  
pp. 13-17
Author(s):  
Y. Shaham ◽  
J. Lelyveld ◽  
U. Marder ◽  
H. Mendelssohn ◽  
G. Paz ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Seminal Vesicle ◽  
Male Fertility ◽  
Reproductive Capacity ◽  
Psammomys Obesus ◽  
Sand Rat ◽  
Testosterone Levels ◽  
Laboratory Colony

The lifespan and fertility of albino sand rats were found to be severely reduced in comparison with a laboratory colony of brown sand rats. The albinos were also much more susceptible to diabetes, as judged by their glucose tolerance. In fact, untreated albinos had a higher incidence of diabetic response than coloured sand rats fed a diabetogenic diet. The albino sand rats reproduced poorly because of a reduction in male fertility. Circulating testosterone levels and seminal vesicle weights were reduced in the albinos. It is speculated that the reduction in reproductive capacity is related to diabetes.

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