Exploring genome-wide datasets of MHC class II antigen presentation

2013 ◽  
Vol 55 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Ruud H. Wijdeven ◽  
Jeroen M. Bakker ◽  
Petra Paul ◽  
Jacques Neefjes
Keyword(s):  
Antigen Presentation ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Genome Wide ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

scholarly journals A Genome-wide Multidimensional RNAi Screen Reveals Pathways Controlling MHC Class II Antigen Presentation

Cell ◽  
2011 ◽  
Vol 145 (2) ◽  
pp. 268-283 ◽  
Author(s):  
Petra Paul ◽  
Tineke van den Hoorn ◽  
Marlieke L.M. Jongsma ◽  
Mark J. Bakker ◽  
Rutger Hengeveld ◽  
...  
Keyword(s):  
Antigen Presentation ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Rnai Screen ◽  
Genome Wide ◽  
A Genome ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

Towards a systems understanding of MHC class I and MHC class II antigen presentation

2011 ◽  
Vol 11 (12) ◽  
pp. 823-836 ◽  
Author(s):  
Jacques Neefjes ◽  
Marlieke L. M. Jongsma ◽  
Petra Paul ◽  
Oddmund Bakke
Keyword(s):  
Antigen Presentation ◽  
Mhc Class I ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Class I ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

scholarly journals Regulation of MHC Class II Antigen Presentation by Sorting of Recycling HLA-DM/DO and Class II within the Multivesicular Body

2001 ◽  
Vol 167 (2) ◽  
pp. 884-892 ◽  
Author(s):  
Marcel van Lith ◽  
Marieke van Ham ◽  
Alexander Griekspoor ◽  
Esther Tjin ◽  
Desiree Verwoerd ◽  
...  
Keyword(s):  
Antigen Presentation ◽  
Mhc Class Ii ◽  
Multivesicular Body ◽  
Class Ii ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

scholarly journals Alternative Endogenous Protein Processing via an Autophagy-Dependent Pathway Compensates for Yersinia-Mediated Inhibition of Endosomal Major Histocompatibility Complex Class II Antigen Presentation

2010 ◽  
Vol 78 (12) ◽  
pp. 5138-5150 ◽  
Author(s):  
Holger Rüssmann ◽  
Klaus Panthel ◽  
Brigitte Köhn ◽  
Stefan Jellbauer ◽  
Sebastian E. Winter ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Antigen Presentation ◽  
Mhc Class Ii ◽  
Virulence Factors ◽  
Antigen Processing ◽  
Class Ii ◽  
T Cell Response ◽  
Histocompatibility Complex ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

ABSTRACT Extracellular Yersinia pseudotuberculosis employs a type III secretion system (T3SS) for translocating virulence factors (Yersinia outer proteins [Yops]) directly into the cytosol of eukaryotic cells. Recently, we used YopE as a carrier molecule for T3SS-dependent secretion and translocation of listeriolysin O (LLO) from Listeria monocytogenes. We demonstrated that translocation of chimeric YopE/LLO into the cytosol of macrophages by Yersinia results in the induction of a codominant antigen-specific CD4 and CD8 T-cell response in orally immunized mice. In this study, we addressed the requirements for processing and major histocompatibility complex (MHC) class II presentation of chimeric YopE proteins translocated into the cytosol of macrophages by the Yersinia T3SS. Our data demonstrate the ability of Yersinia to counteract exogenous MHC class II antigen presentation of secreted hybrid YopE by the action of wild-type YopE and YopH. In the absence of exogenous MHC class II antigen presentation, an alternative pathway was identified for YopE fusion proteins originating in the cytosol. This endogenous antigen-processing pathway was sensitive to inhibitors of phagolysosomal acidification and macroautophagy, but it did not require the function either of the proteasome or of transporters associated with antigen processing. Thus, by an autophagy-dependent mechanism, macrophages are able to compensate for the YopE/YopH-mediated inhibition of the endosomal MHC class II antigen presentation pathway for exogenous antigens. This is the first report demonstrating that autophagy might enable the host to mount an MHC class II-restricted CD4 T-cell response against translocated bacterial virulence factors. We provide critical new insights into the interaction between the mammalian immune system and a human pathogen.

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