scholarly journals RPA Phosphorylation Inhibits DNA Resection

2019 ◽  
Vol 75 (1) ◽  
pp. 145-153.e5 ◽  
Author(s):  
Michael M. Soniat ◽  
Logan R. Myler ◽  
Hung-Che Kuo ◽  
Tanya T. Paull ◽  
Ilya J. Finkelstein
Keyword(s):  
Dna Resection

scholarly journals MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading

2020 ◽  
Vol 77 (2) ◽  
pp. 395-410.e3 ◽  
Author(s):  
Axel Delamarre ◽  
Antoine Barthe ◽  
Christophe de la Roche Saint-André ◽  
Pierre Luciano ◽  
Romain Forey ◽  
...  
Keyword(s):  
Chromatin Accessibility ◽  
Replication Forks ◽  
Stalled Replication Forks ◽  
Dna Resection

scholarly journals Human BRCA1–BARD1 ubiquitin ligase activity counteracts chromatin barriers to DNA resection

2016 ◽  
Vol 23 (7) ◽  
pp. 647-655 ◽  
Author(s):  
Ruth M Densham ◽  
Alexander J Garvin ◽  
Helen R Stone ◽  
Joanna Strachan ◽  
Robert A Baldock ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Ubiquitin Ligase Activity ◽  
Dna Resection

scholarly journals The DNA resection protein CtIP promotes mammary tumorigenesis

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32172-32183 ◽  
Author(s):  
Colleen R. Reczek ◽  
Reena Shakya ◽  
Yana Miteva ◽  
Matthias Szabolcs ◽  
Thomas Ludwig ◽  
...  
Keyword(s):  
Mammary Tumorigenesis ◽  
Dna Resection

scholarly journals Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks

2015 ◽  
Vol 44 (2) ◽  
pp. 669-682 ◽  
Author(s):  
Diego Dibitetto ◽  
Matteo Ferrari ◽  
Chetan C. Rawal ◽  
Attila Balint ◽  
TaeHyung Kim ◽  
...  
Keyword(s):  
Double Strand Breaks ◽  
Strand Breaks ◽  
Dna Resection

scholarly journals Coordinated nuclease activities counteract Ku at single-ended DNA double-strand breaks

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Pauline Chanut ◽  
Sébastien Britton ◽  
Julia Coates ◽  
Stephen P. Jackson ◽  
Patrick Calsou
Keyword(s):  
Homologous Recombination ◽  
Mammalian Cells ◽  
Double Strand Breaks ◽  
Exonuclease Activity ◽  
Endonuclease Activity ◽  
Dna Double Strand Breaks ◽  
Additional Mechanism ◽  
Strand Breaks ◽  
Rad51 Focus ◽  
Dna Resection

Abstract Repair of single-ended DNA double-strand breaks (seDSBs) by homologous recombination (HR) requires the generation of a 3′ single-strand DNA overhang by exonuclease activities in a process called DNA resection. However, it is anticipated that the highly abundant DNA end-binding protein Ku sequesters seDSBs and shields them from exonuclease activities. Despite pioneering works in yeast, it is unclear how mammalian cells counteract Ku at seDSBs to allow HR to proceed. Here we show that in human cells, ATM-dependent phosphorylation of CtIP and the epistatic and coordinated actions of MRE11 and CtIP nuclease activities are required to limit the stable loading of Ku on seDSBs. We also provide evidence for a hitherto unsuspected additional mechanism that contributes to prevent Ku accumulation at seDSBs, acting downstream of MRE11 endonuclease activity and in parallel with MRE11 exonuclease activity. Finally, we show that Ku persistence at seDSBs compromises Rad51 focus assembly but not DNA resection.

scholarly journals Mechanism of DNA resection during intrachromosomal recombination and immunoglobulin class switching

2012 ◽  
Vol 199 (7) ◽  
pp. i11-i11
Author(s):  
Anne Bothmer ◽  
Philipp C. Rommel ◽  
Anna Gazumyan ◽  
Federica Polato ◽  
Colleen R. Reczek ◽  
...  
Keyword(s):  
Class Switching ◽  
Immunoglobulin Class ◽  
Intrachromosomal Recombination ◽  
Immunoglobulin Class Switching ◽  
Dna Resection
Sign in / Sign up

Export Citation Format

Share Document