scholarly journals Transcriptome Maps of mRNP Biogenesis Factors Define Pre-mRNA Recognition

2014 ◽  
Vol 55 (5) ◽  
pp. 745-757 ◽  
Author(s):  
Carlo Baejen ◽  
Phillipp Torkler ◽  
Saskia Gressel ◽  
Katharina Essig ◽  
Johannes Söding ◽  
...  
Keyword(s):  
Mrnp Biogenesis

scholarly journals The THP1-SAC3-SUS1-CDC31 Complex Works in Transcription Elongation-mRNA Export Preventing RNA-mediated Genome Instability

2008 ◽  
Vol 19 (10) ◽  
pp. 4310-4318 ◽  
Author(s):  
Cristina González-Aguilera ◽  
Cristina Tous ◽  
Belén Gómez-González ◽  
Pablo Huertas ◽  
Rosa Luna ◽  
...  
Keyword(s):  
Genome Instability ◽  
Cytidine Deaminase ◽  
Transcription Elongation ◽  
Mrna Export ◽  
Feedback Mechanism ◽  
Rnase H ◽  
Genome Integrity ◽  
Activation Induced Cytidine Deaminase ◽  
Specific Pathway ◽  
Mrnp Biogenesis

The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions.

scholarly journals Nucleocytoplasmic mRNP export is an integral part of mRNP biogenesis

Chromosoma ◽  
2010 ◽  
Vol 120 (1) ◽  
pp. 23-38 ◽  
Author(s):  
Petra Björk ◽  
Lars Wieslander
Keyword(s):  
Mrnp Biogenesis

scholarly journals Expression of Bacterial Rho Factor in Yeast Identifies New Factors Involved in the Functional Interplay between Transcription and mRNP Biogenesis

2009 ◽  
Vol 29 (15) ◽  
pp. 4033-4044 ◽  
Author(s):  
Christine Mosrin-Huaman ◽  
Romy Honorine ◽  
A. Rachid Rahmouni
Keyword(s):  
Rna Polymerase Ii ◽  
Growth Defect ◽  
Large Set ◽  
Mrna Synthesis ◽  
Ribonucleoprotein Particle ◽  
Nuclear Exosome ◽  
Dependent Growth ◽  
Dose Dependent ◽  
Dynamic Interplay ◽  
Mrnp Biogenesis

ABSTRACT In eukaryotic cells, the nascent pre-mRNA molecule is coated sequentially with a large set of processing and binding proteins that mediate its transformation into an export-competent ribonucleoprotein particle (mRNP) that is ready for translation in the cytoplasm. We have implemented an original assay that monitors the dynamic interplay between transcription and mRNP biogenesis and that allows the screening for new factors linking mRNA synthesis to translation in Saccharomyces cerevisiae. The assay is based on the perturbation of gene expression induced by the bacterial Rho factor, an RNA-dependent helicase/translocase that acts as a competitor at one or several steps of mRNP biogenesis in yeast. We show that the expression of Rho in yeast leads to a dose-dependent growth defect that stems from its action on RNA polymerase II-mediated transcription. Rho expression induces the production of aberrant transcripts that are degraded by the nuclear exosome. A screen for dosage suppressors of the Rho-induced growth defect identified several genes that are involved in the different steps of mRNP biogenesis and export, as well as other genes with both known functions in transcription regulation and unknown functions. Our results provide evidence for an extensive cross talk between transcription, mRNP biogenesis, and export. They also uncover new factors that potentially are involved in these interconnected events.

scholarly journals Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
María S Domínguez-Sánchez ◽  
Carmen Sáez ◽  
Miguel A Japón ◽  
Andrés Aguilera ◽  
Rosa Luna
Keyword(s):  
Differential Expression ◽  
Human Cancers ◽  
Mrnp Biogenesis

scholarly journals Tho1, a Novel hnRNP, and Sub2 Provide Alternative Pathways for mRNP Biogenesis in Yeast THO Mutants

2006 ◽  
Vol 26 (12) ◽  
pp. 4387-4398 ◽  
Author(s):  
Sonia Jimeno ◽  
Rosa Luna ◽  
María García-Rubio ◽  
Andrés Aguilera
Keyword(s):  
Nuclear Protein ◽  
Rna Binding ◽  
Binding Activity ◽  
Dependent Manner ◽  
Mrna Accumulation ◽  
Alternative Pathways ◽  
Multicopy Suppressors ◽  
Rna Export ◽  
Nascent Mrna ◽  
Mrnp Biogenesis

ABSTRACT THO is a protein complex that functions in cotranscriptional mRNP formation. Yeast THO1 and SUB2 (Saccharomyces cerevisiae) were identified as multicopy suppressors of the expression defects of the hpr1Δ mutant of THO. Here we show that multicopy THO1 suppresses the mRNA accumulation and export defects and the hyperrecombination phenotype of THO mutants but not those of sub2Δ, thp1Δ, or spt4Δ. Similarly, Sub2 overexpression suppresses the RNA export defect of hpr1Δ. Tho1 is a conserved RNA binding nuclear protein that specifically binds to transcribed chromatin in a THO- and RNA-dependent manner and genetically interacts with the shuttling hnRNP Nab2. The ability of Tho1 to suppress hpr1Δ resides in its C-terminal half, which contains the RNA binding activity and is located after a SAP/SAF (scaffold-associated protein/scaffold-associated factor) domain. Altogether, these results suggest that Tho1 is an hnRNP that, similarly to Sub2, assembles onto the nascent mRNA during transcription and participates in mRNP biogenesis and export. Overexpression of Tho1 or Sub2 may provide alternative ways for mRNP formation and export in the absence of a functional THO complex.

scholarly journals The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis

Cell ◽  
2016 ◽  
Vol 167 (5) ◽  
pp. 1201-1214.e15 ◽  
Author(s):  
Anna Babour ◽  
Qingtang Shen ◽  
Julien Dos-Santos ◽  
Struan Murray ◽  
Alexandre Gay ◽  
...  
Keyword(s):  
Quality Control ◽  
Control Factor ◽  
Chromatin Remodeler ◽  
Mrnp Biogenesis

scholarly journals Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors

BMC Genetics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 80 ◽  
Author(s):  
Francisco Estruch ◽  
Christine Hodge ◽  
Natalia Gómez-Navarro ◽  
Lorena Peiró-Chova ◽  
Catherine V Heath ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Genetic Interactions ◽  
Mrnp Biogenesis
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