scholarly journals Polypyrimidine Tract Binding Protein Stabilizes the Encephalomyocarditis Virus IRES Structure via Binding Multiple Sites in a Unique Orientation

2009 ◽  
Vol 34 (5) ◽  
pp. 556-568 ◽  
Author(s):  
Panagiota Kafasla ◽  
Nina Morgner ◽  
Tuija A.A. Pöyry ◽  
Stephen Curry ◽  
Carol V. Robinson ◽  
...  
Keyword(s):  
Binding Protein ◽  
Encephalomyocarditis Virus ◽  
Polypyrimidine Tract ◽  
Polypyrimidine Tract Binding ◽  
Polypyrimidine Tract Binding Protein

scholarly journals The Polypyrimidine Tract Binding Protein Is Required for Efficient Picornavirus Gene Expression and Propagation

2005 ◽  
Vol 79 (10) ◽  
pp. 6172-6179 ◽  
Author(s):  
Paola M. Florez ◽  
October M. Sessions ◽  
Eric J. Wagner ◽  
Matthias Gromeier ◽  
Mariano A. Garcia-Blanco
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Binding Protein ◽  
Viral Gene Expression ◽  
Encephalomyocarditis Virus ◽  
Mammalian Host ◽  
Viral Gene ◽  
Polypyrimidine Tract ◽  
Polypyrimidine Tract Binding ◽  
Polypyrimidine Tract Binding Protein

ABSTRACT Mammalian host factors required for efficient viral gene expression and propagation have been often recalcitrant to genetic analysis. A case in point is the function of cellular factors that trans-activate internal ribosomal entry site (IRES)-driven translation, which is operative in many positive-stranded RNA viruses, including all picornaviruses. These IRES trans-acting factors have been elegantly studied in vitro, but their in vivo importance for viral gene expression and propagation has not been widely confirmed experimentally. Here we use RNA interference to deplete mammalian cells of one such factor, the polypyrimidine tract binding protein, and test its requirement in picornavirus gene expression and propagation. Depletion of the polypyrimidine tract binding protein resulted in a marked delay of particle propagation and significantly decreased synthesis and accumulation of viral proteins of poliovirus and encephalomyocarditis virus. These effects could be partially restored by expression of an RNA interference-resistant exogenous polypyrimidine tract binding protein. These data indicate a critical role for the polypyrimidine tract binding protein in picornavirus gene expression and strongly suggest a requirement for efficient IRES-dependent translation.

scholarly journals Polypyrimidine Tract Binding Protein Modulates Efficiency of Polyadenylation

2004 ◽  
Vol 24 (15) ◽  
pp. 6889-6889 ◽  
Author(s):  
Pedro Castelo-Branco ◽  
Andre Furger ◽  
Matthew Wollerton ◽  
Christopher Smith ◽  
Alexandra Moreira ◽  
...  
Keyword(s):  
Binding Protein ◽  
Polypyrimidine Tract ◽  
Polypyrimidine Tract Binding ◽  
Polypyrimidine Tract Binding Protein
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