scholarly journals CED-9 and EGL-1: A Duo Also Regulating Mitochondrial Network Morphology

2006 ◽  
Vol 21 (6) ◽  
pp. 730-732 ◽  
Author(s):  
Jérôme Estaquier ◽  
Damien Arnoult
Keyword(s):  
Mitochondrial Network ◽  
Network Morphology

Analysis of Mitochondrial Network Morphology in Cultured Myoblasts from Patients with Mitochondrial Disorders

2015 ◽  
Vol 39 (5) ◽  
pp. 340-350 ◽  
Author(s):  
J. Sládková ◽  
J. Spáčilová ◽  
M. Čapek ◽  
M. Tesařová ◽  
H. Hansíková ◽  
...  
Keyword(s):  
Mitochondrial Disorders ◽  
Mitochondrial Network ◽  
Network Morphology

scholarly journals Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line

Viruses ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 266 ◽  
Author(s):  
Karolina Gregorczyk ◽  
Zbigniew Wyżewski ◽  
Joanna Szczepanowska ◽  
Felix Toka ◽  
Matylda Mielcarska ◽  
...  
Keyword(s):  
Cell Line ◽  
Mitochondrial Network ◽  
Macrophage Cell Line ◽  
Ectromelia Virus ◽  
Macrophage Cell ◽  
Murine Fibroblasts ◽  
Network Morphology

A simple ImageJ macro tool for analyzing mitochondrial network morphology in mammalian cell culture

2017 ◽  
Vol 119 (3) ◽  
pp. 315-326 ◽  
Author(s):  
Andrew J. Valente ◽  
Lucas A. Maddalena ◽  
Ellen L. Robb ◽  
Fereshteh Moradi ◽  
Jeffrey A. Stuart
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Mitochondrial Network ◽  
Network Morphology

scholarly journals Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 643
Author(s):  
Iwona Lasocka ◽  
Lidia Szulc-Dąbrowska ◽  
Michał Skibniewski ◽  
Ewa Skibniewska ◽  
Karolina Gregorczyk-Zboroch ◽  
...  
Keyword(s):  
Glass Slide ◽  
Mitochondrial Morphology ◽  
Graphene Monolayer ◽  
Fluorescence Intensity Ratio ◽  
Mitochondrial Network ◽  
Glass Cover ◽  
3T3 Fibroblasts ◽  
Focal Contacts ◽  
Cover Slide ◽  
Network Morphology

This study investigates the effect of graphene scaffold on morphology, viability, cytoskeleton, focal contacts, mitochondrial network morphology and activity in BALB/3T3 fibroblasts and provides new data on biocompatibility of the “graphene-family nanomaterials”. We used graphene monolayer applied onto glass cover slide by electrochemical delamination method and regular glass cover slide, as a reference. The morphology of fibroblasts growing on graphene was unaltered, and the cell viability was 95% compared to control cells on non-coated glass slide. There was no significant difference in the cell size (spreading) between both groups studied. Graphene platform significantly increased BALB/3T3 cell mitochondrial activity (WST-8 test) compared to glass substrate. To demonstrate the variability in focal contacts pattern, the effect of graphene on vinculin was examined, which revealed a significant increase in focal contact size comparing to control-glass slide. There was no disruption in mitochondrial network morphology, which was branched and well connected in relation to the control group. Evaluation of the JC-1 red/green fluorescence intensity ratio revealed similar levels of mitochondrial membrane potential in cells growing on graphene-coated and uncoated slides. These results indicate that graphene monolayer scaffold is cytocompatible with connective tissue cells examined and could be beneficial for tissue engineering therapy.

scholarly journals Mitochondria reorganization upon proliferation arrest predicts individual yeast cell fate

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Damien Laporte ◽  
Laëtitia Gouleme ◽  
Laure Jimenez ◽  
Ines Khemiri ◽  
Isabelle Sagot
Keyword(s):  
Yeast Cell ◽  
Cell Fate ◽  
Individual Cell ◽  
Population Based ◽  
Yeast Cells ◽  
Mitochondrial Network ◽  
Cell Level ◽  
Quiescent Cells ◽  
The Individual ◽  
Network Morphology

Most cells spend the majority of their life in a non-proliferating state. When proliferation cessation is irreversible, cells are senescent. By contrast, if the arrest is only temporary, cells are defined as quiescent. These cellular states are hardly distinguishable without triggering proliferation resumption, hampering thus the study of quiescent cells properties. Here we show that quiescent and senescent yeast cells are recognizable based on their mitochondrial network morphology. Indeed, while quiescent yeast cells display numerous small vesicular mitochondria, senescent cells exhibit few globular mitochondria. This allowed us to reconsider at the individual-cell level, properties previously attributed to quiescent cells using population-based approaches. We demonstrate that cell’s propensity to enter quiescence is not influenced by replicative age, volume or density. Overall, our findings reveal that quiescent cells are not all identical but that their ability to survive is significantly improved when they exhibit the specific reorganization of several cellular machineries.

Altered mitochondrial network morphology and regulatory proteins in mitochondrial quality control in myotubes from severely obese humans with or without type 2 diabetes

2020 ◽  
Vol 45 (3) ◽  
pp. 283-293
Author(s):  
Anders E. Gundersen ◽  
Benjamin A. Kugler ◽  
Paul M. McDonald ◽  
Alexey Veraksa ◽  
Joseph A. Houmard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Mitochondrial Fission ◽  
Mitochondrial Network ◽  
Mitochondrial Quality Control ◽  
Control Processes ◽  
Severely Obese ◽  
Type 2 Diabetic ◽  
Network Morphology

Healthy mitochondrial networks are maintained via balanced integration of mitochondrial quality control processes (biogenesis, fusion, fission, and mitophagy). The purpose of this study was to investigate the effects of severe obesity and type 2 diabetes (T2D) on mitochondrial network morphology and expression of proteins regulating mitochondrial quality control processes in cultured human myotubes. Primary human skeletal muscle cells were isolated from biopsies from lean, severely obese nondiabetic individuals and severely obese type 2 diabetic individuals (n = 8–9/group) and were differentiated to myotubes. Mitochondrial network morphology was determined in live cells via confocal microscopy and protein markers of mitochondrial quality control were measured by immunoblotting. Myotubes from severely obese nondiabetic and type 2 diabetic humans exhibited fragmented mitochondrial networks (P < 0.05). Mitochondrial fission protein Drp1 (Ser616) phosphorylation was higher in myotubes from severely obese nondiabetic humans when compared with the lean controls (P < 0.05), while mitophagy protein Parkin expression was lower in myotubes from severely obese individuals with T2D in comparison to the other groups (P < 0.05). These data suggest that regulatory proteins in mitochondrial quality control processes, specifically mitochondrial fission protein Drp1 (Ser616) phosphorylation and mitophagy protein Parkin, are intrinsically dysregulated at cellular level in skeletal muscle from severely obese nondiabetic and type 2 diabetic humans, respectively. These differentially expressed mitochondrial quality control proteins may play a role in mitochondrial fragmentation evident in skeletal muscle from severely obese and type 2 diabetic humans. Novelty Mitochondrial network morphology and mitochondrial quality control proteins are intrinsically dysregulated in skeletal muscle cells from severely obese humans with or without T2D.

Freeze-etch TEM of aqueous polymer gel network morphology

1986 ◽  
Vol 44 ◽  
pp. 796-797
Author(s):  
J. A. N. Zasadzinski ◽  
R. K. Prud'homme
Keyword(s):  
Metal Ion ◽  
Polymer Network ◽  
Polymer Gels ◽  
Polymer Gel ◽  
Deformation History ◽  
Crosslinked Polymer ◽  
Aqueous Polymer ◽  
History Of ◽  
Gel Network ◽  
Network Morphology

The rheological and mechanical properties of crosslinked polymer gels arise from the structure of the gel network. In turn, the structure of the gel network results from: thermodynamically determined interactions between the polymer chain segments, the interactions of the crosslinking metal ion with the polymer, and the deformation history of the network. Interpretations of mechanical and rheological measurements on polymer gels invariably begin with a conceptual model of,the microstructure of the gel network derived from polymer kinetic theory. In the present work, we use freeze-etch replication TEM to image the polymer network morphology of titanium crosslinked hydroxypropyl guars in an attempt to directly relate macroscopic phenomena with network structure.

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