scholarly journals Conserved Translational Frameshift in dsDNA Bacteriophage Tail Assembly Genes

2004 ◽  
Vol 16 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Jun Xu ◽  
Roger W. Hendrix ◽  
Robert L. Duda
Keyword(s):  
Translational Frameshift ◽  
Tail Assembly

A Programmed Translational Frameshift is Required for the Synthesis of a Bacteriophage λ Tail Assembly Protein

1993 ◽  
Vol 234 (1) ◽  
pp. 124-139 ◽  
Author(s):  
Margaret E. Levin ◽  
Roger W. Hendrix ◽  
Sherwood R. Casjens
Keyword(s):  
Bacteriophage Λ ◽  
Translational Frameshift ◽  
Tail Assembly

scholarly journals Fluorescent T7 display phages obtained by translational frameshift

2006 ◽  
Vol 34 (20) ◽  
pp. e137-e137 ◽  
Author(s):  
Erik J. Slootweg ◽  
Hans J.H.G. Keller ◽  
Mark A. Hink ◽  
Jan Willem Borst ◽  
Jaap Bakker ◽  
...  
Keyword(s):  
Translational Frameshift

scholarly journals Study and characterization of tobacco mosaic virus head-to-tail assembly assisted by aniline polymerization

2006 ◽  
pp. 3019 ◽  
Author(s):  
Zhongwei Niu ◽  
Michael Bruckman ◽  
Venkata S. Kotakadi ◽  
Jinbo He ◽  
Todd Emrick ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Tail Assembly ◽  
Aniline Polymerization

Identification of a tail assembly gene cluster from deep-sea thermophilic bacteriophage GVE2

Virus Genes ◽  
2009 ◽  
Vol 38 (3) ◽  
pp. 507-514 ◽  
Author(s):  
Suijie Wu ◽  
Bin Liu ◽  
Xiaobo Zhang
Keyword(s):  
Gene Cluster ◽  
Deep Sea ◽  
Tail Assembly ◽  
Thermophilic Bacteriophage

scholarly journals Bacteriophage T4 Self-Assembly: Localization of gp3 and Its Role in Determining Tail Length

2000 ◽  
Vol 182 (3) ◽  
pp. 680-688 ◽  
Author(s):  
A. Vianelli ◽  
G. R. Wang ◽  
M. Gingery ◽  
R. L. Duda ◽  
F. A. Eiserling ◽  
...  
Keyword(s):  
Self Assembly ◽  
High Efficiency ◽  
Bacteriophage T4 ◽  
Tail Length ◽  
Hypothetical Protein ◽  
Protein Product ◽  
Amino Acid Sequences ◽  
Tail Assembly ◽  
Infected Cells ◽  
Assembly Intermediate

ABSTRACT Gene 3 of bacteriophage T4 participates at a late stage in the T4 tail assembly pathway, but the hypothetical protein product, gp3, has never been identified in extracts of infected cells or in any tail assembly intermediate. In order to overcome this difficulty, we expressed gp3 in a high-efficiency plasmid expression vector and subsequently purified it for further analysis. The N-terminal sequence of the purified protein showed that the initial methionine had been removed. Variant C-terminal amino acid sequences were resolved by determining the cysteine content of the protein. The molecular mass of 20.6 kDa for the pure protein was confirmed by Western blotting, using a specific anti-gp3 serum for which the purified protein was the immunogen. We also demonstrated, for the first time, the physical presence of gp3 in the mature T4 phage particle and localized it to the tail tube. By finding a nonleaky, nonpermissive host for a gene 3 mutant, we could clearly demonstrate a new phenotype: the slow, aberrant elongation of the tail tube in the absence of gp3.

scholarly journals Programmed Translational Frameshift in the Bacteriophage P2 FETUD Tail Gene Operon

2002 ◽  
Vol 184 (23) ◽  
pp. 6522-6531 ◽  
Author(s):  
Gail E. Christie ◽  
Louise M. Temple ◽  
Becky A. Bartlett ◽  
Tina S. Goodwin
Keyword(s):  
Gene Cluster ◽  
Sequence Similarity ◽  
Initiation Site ◽  
Translation Initiation Site ◽  
Amino Acid Sequence Similarity ◽  
Reading Frame ◽  
Coding Regions ◽  
Translational Frameshift ◽  
Bacteriophage P2 ◽  
Contractile Tail

ABSTRACT The major structural components of the P2 contractile tail are encoded in the FETUD tail gene operon. The sequences of genes F I and F II, encoding the major tail sheath and tail tube proteins, have been reported previously (L. M. Temple, S. L. Forsburg, R. Calendar, and G. E. Christie, Virology 181:353-358, 1991). Sequence analysis of the remainder of this operon and the locations of amber mutations Eam30, Tam5, Tam64, Tam215, Uam25, Uam77, Uam92, and Dam6 and missense mutation Ets55 identified the coding regions for genes E, T, U, and D, completing the sequence determination of the P2 genome. Inspection of the DNA sequence revealed a new open reading frame overlapping the end of the essential tail gene E. Lack of an apparent translation initiation site and identification of a putative sequence for a programmed translational frameshift within the E gene suggested that this new reading frame (E′) might be translated as an extension of gene E, following a −1 translational frameshift. Complementation analysis demonstrated that E′ was essential for P2 lytic growth. Analysis of fusion polypeptides verified that this reading frame was translated as a −1 frameshift extension of gpE, with a frequency of approximately 10%. The arrangement of these two genes within the tail gene cluster of phage P2 and their coupling via a translational frameshift appears to be conserved among P2-related phages. This arrangement shows a striking parallel to the organization in the tail gene cluster of phage lambda, despite a lack of amino acid sequence similarity between the tail gene products of these phage families.

scholarly journals Structure and Mechanism of Dimer–Monomer Transition of a Plant Poly(A)-Binding Protein upon RNA Interaction: Insights into Its Poly(A) Tail Assembly

2015 ◽  
Vol 427 (15) ◽  
pp. 2491-2506 ◽  
Author(s):  
Mariane Noronha Domingues ◽  
Mauricio Luis Sforça ◽  
Adriana Santos Soprano ◽  
Jack Lee ◽  
Tatiana de Arruda Campos Brasil de Souza ◽  
...  
Keyword(s):  
Binding Protein ◽  
Tail Assembly ◽  
Rna Interaction

scholarly journals Experimental Joint Identification Using System Equivalent Model Mixing in a Bladed Disk

2020 ◽  
Vol 142 (5) ◽  
Author(s):  
Zeeshan Saeed ◽  
Steven W. B. Klaassen ◽  
Christian M. Firrone ◽  
Teresa M. Berruti ◽  
Daniel J. Rixen
Keyword(s):  
Degrees Of Freedom ◽  
Three Dimensional ◽  
Equivalent Model ◽  
Dynamic Information ◽  
Response Measurement ◽  
Decoupling Method ◽  
Tail Assembly ◽  
Joint Dynamics ◽  
Model Mixing ◽  
Joint Identification

Abstract A joint between two components can be seen as a means to transmit dynamic information from one side to the other. To identify the joint, a reverse process called decoupling can be applied. This is not as straightforward as the coupling, especially when the substructures have three-dimensional characteristics, or sensor mounting effects are significant, or the interface degrees-of-freedom (DoF) are inaccessible for response measurement and excitation. Acquiring frequency response functions (FRFs) at the interface DoF, therefore, becomes challenging. Consequently, one has to consider hybrid or expansion methods that can expand the observed dynamics on accessible DoF to inaccessible DoF. In this work, we attempt to identify the joint dynamics using the system equivalent model mixing (SEMM) decoupling method with a virtual point description of the interface. Measurements are made only at the internal DoF of the uncoupled substructures and also of the coupled structure assuming that the joint dynamics are observable in the assembled state. Expanding them to the interface DoF and performing coupling and decoupling operations iteratively, the joint is identified. The substructures under consideration are a disk and blade—an academic test geometry that has a total of 18 blades but only one blade-to-disk joint is considered in this investigation. The joint is a typical dove-tail assembly. The method is shown to identify the joint without any direct interface DoF measurement.

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