scholarly journals Arginine Methylation of NIP45 Modulates Cytokine Gene Expression in Effector T Lymphocytes

2004 ◽  
Vol 15 (4) ◽  
pp. 559-571 ◽  
Author(s):  
Kerri A. Mowen ◽  
Brandon T. Schurter ◽  
John W. Fathman ◽  
Michael David ◽  
Laurie H. Glimcher
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Arginine Methylation ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

scholarly journals Epigenetic Regulation of Cytokine Gene Expression in T Lymphocytes

2009 ◽  
Vol 50 (3) ◽  
pp. 322 ◽  
Author(s):  
Choong-Gu Lee ◽  
Anupama Sahoo ◽  
Sin-Hyeog Im
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Epigenetic Regulation ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

scholarly journals Patterns of cytokine gene expression of naı̈ve and memory T lymphocytes in vivo

2002 ◽  
Vol 87 (3-4) ◽  
pp. 261-264 ◽  
Author(s):  
Anton G Gossner ◽  
Samantha Bailey ◽  
Nora Hunter ◽  
John Hopkins
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Memory T Lymphocytes

Altered CD3 chain and cytokine gene expression in tumor infiltrating T lymphocytes during the development of mesothelioma

1996 ◽  
Vol 103 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Andrew G. Jarnicki ◽  
David R. Fitzpatrick ◽  
Bruce W.S. Robinson ◽  
Helle Bielefeldt-Ohmann
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Cytokine Gene Expression ◽  
Cytokine Gene

Receptor Signal Transduction and Cytokine Gene Expression in T Lymphocytes

2015 ◽  
pp. 79-91
Author(s):  
Roberto Testi ◽  
Daniele D�Ambrosio ◽  
Rossana Trotta ◽  
Ruggero DeMaria ◽  
Angela Santoni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
T Lymphocytes ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Receptor Signal

scholarly journals Arginine methylation regulates IL-2 gene expression: a role for protein arginine methyltransferase 5 (PRMT5)

2005 ◽  
Vol 388 (1) ◽  
pp. 379-386 ◽  
Author(s):  
Stéphane RICHARD ◽  
Mélanie MOREL ◽  
Patrick CLÉROUX
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
T Cell Activation ◽  
Cell Activation ◽  
Arginine Methylation ◽  
Cytokine Gene Expression ◽  
Luciferase Reporter ◽  
Protein Arginine Methylation

Arginine methylation is a post-translational modification resulting in the generation of aDMAs (asymmetrical ω-NG, NG-dimethylated arginines) and sDMAs (symmetrical ω-NG, N′G-dimethylated arginines). The role of arginine methylation in cell signalling and gene expression in T lymphocytes is not understood. In the present study, we report a role for protein arginine methylation in regulating IL-2 (interleukin 2) gene expression in T lymphocytes. Leukaemic Jurkat T-cells treated with a known methylase inhibitor, 5′-methylthioadenosine, had decreased cytokine gene expression, as measured using an NF-AT (nuclear factor of activated T-cells)-responsive promoter linked to the luciferase reporter gene. Since methylase inhibitors block all methylation events, we performed RNA interference with small interfering RNAs against the major PRMT (protein arginine methyltransferases) that generates sDMA (PRMT5). The dose-dependent decrease in PRMT5 expression resulted in the inhibition of both IL-2- and NF-AT-driven promoter activities and IL-2 secretion. By using an sDMA-specific antibody, we observed that sDMA-containing proteins are directly associated with the IL-2 promoter after T-cell activation. Since changes in protein arginine methylation were not observed after T-cell activation in Jurkat and human peripheral blood lymphocytes, our results demonstrate that it is the recruitment of methylarginine-specific protein(s) to cytokine promoter regions that regulates their gene expression.

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