scholarly journals Evaluation of accelerated Cellular Senescence in Mesenchymal Stem Cells Isolated from Human Bone Marrow, Adipose Tissue, UCB and wharton’s Jelly

2017 ◽  
Vol 145 ◽  
pp. S172
Author(s):  
Sivamani Ganesan ◽  
S. Vasanthakumar
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cellular Senescence ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Wharton's Jelly ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue

scholarly journals Comparison between Mesenchymal Stem Cells obtained from Bone Marrow, Adipose Tissue and Wharton Gelatin according to the ISCT Criteria

2017 ◽  
Author(s):  
◽  
A. Parra-Barrera
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cellular Therapy ◽  
Wharton’S Jelly ◽  
Alternative Source ◽  
Wharton's Jelly ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue

Mesenchymal stem cells (MSC) represent a heterogeneous population with the capacity to self-renew and differentiate into different cell types. At the middle of the last century these cells initially were described in bone marrow (BM), thence this tissue has become the gold standard for obtaining and characterization of MSC. It is known that these cells are housed in specific areas called niches distributed throughout all body, where they contribute to tissue regeneration processes of self-tissue were they are located. However, finding an alternative source of CTM with the same characteristics that have showed in MO, but its obtention no represent a risk since the donor is essential to their use for therapeutic purposes. In this study we isolated mesenchymal stem cells from bone marrow, adipose tissue and Wharton’s jelly and they were compared in their characteristics in according to the standards of the International Society for Cellular Therapy (ISCT). The results showed that the morphology as well as adipogenic and osteogenic differentiation and also the expression of surface antigens (CD90, CD73, and CD105) from all tissues accomplished the standards, although Wharton’s jelly represented the best option.

Comparison between Mesenchymal Stem Cells obtained from Bone Marrow, Adipose Tissue and Wharton Gelatin according to the ISCT Criteria

2017 ◽  
Author(s):  
◽  
A. Parra-Barrera ◽  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cellular Therapy ◽  
Wharton’S Jelly ◽  
Alternative Source ◽  
Wharton's Jelly ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue

Mesenchymal stem cells (MSC) represent a heterogeneous population with the capacity to self-renew and differentiate into different cell types. At the middle of the last century these cells initially were described in bone marrow (BM), thence this tissue has become the gold standard for obtaining and characterization of MSC. It is known that these cells are housed in specific areas called niches distributed throughout all body, where they contribute to tissue regeneration processes of self-tissue were they are located. However, finding an alternative source of CTM with the same characteristics that have showed in MO, but its obtention no represent a risk since the donor is essential to their use for therapeutic purposes. In this study we isolated mesenchymal stem cells from bone marrow, adipose tissue and Wharton’s jelly and they were compared in their characteristics in according to the standards of the International Society for Cellular Therapy (ISCT). The results showed that the morphology as well as adipogenic and osteogenic differentiation and also the expression of surface antigens (CD90, CD73, and CD105) from all tissues accomplished the standards, although Wharton’s jelly represented the best option.

scholarly journals Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton’s Jelly

2021 ◽  
Vol 22 (16) ◽  
pp. 8987
Author(s):  
Cheng-Fong Chen ◽  
Yi-Chun Chen ◽  
Yu-Show Fu ◽  
Shang-Wen Tsai ◽  
Po-Kuei Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Wharton’S Jelly ◽  
Allogeneic Bone ◽  
Wharton's Jelly ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Osteochondral Regeneration

Allogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchymal stem cells (hMSCs) for osteochondral regeneration is missing. The hMSCs derived from bone marrow, adipose tissue, and Wharton’s jelly (BMMSCs, ADMSCs, and UMSCs, respectively) are potential candidates for bone regeneration. This study characterized the potential of hDCB as a scaffold for osteogenesis and chondrogenesis of BMMSCs, ADMSCs, and UMSCs. The pore sizes and mechanical strength of hDCB were characterized. Cell survival and adhesion of hMSCs were investigated using MTT assay and F-actin staining. Alizarin Red S and Safranin O staining were conducted to demonstrate calcium deposition and proteoglycan production of hMSCs after osteogenic and chondrogenic differentiation, respectively. A RT-qPCR was performed to analyze the expression levels of osteogenic and chondrogenic markers in hMSCs. Results indicated that BMMSCs and ADMSCs exhibited higher osteogenic potential than UMSCs. Furthermore, ADMSCs and UMSCs had higher chondrogenic potential than BMMSCs. This study demonstrated that chondrogenic ADMSCs- or UMSCs-seeded hDCB might be potential osteochondral constructs for osteochondral regeneration.

Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood

2005 ◽  
Vol 33 (11) ◽  
pp. 1402-1416 ◽  
Author(s):  
Wolfgang Wagner ◽  
Frederik Wein ◽  
Anja Seckinger ◽  
Maria Frankhauser ◽  
Ute Wirkner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Adipose Tissue

Comparative Analysis of Chemokine Receptor's Expression in Mesenchymal Stem Cells Derived from Human Bone Marrow and Adipose Tissue

2010 ◽  
Vol 44 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Naghmeh Ahmadian kia ◽  
Ahmad Reza Bahrami ◽  
Marzieh Ebrahimi ◽  
Maryam M. Matin ◽  
Zeinab Neshati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Comparative Analysis ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential

2014 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Danielle Barberini ◽  
Natália Pereira Freitas ◽  
Mariana Magnoni ◽  
Leandro Maia ◽  
Amanda Listoni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Differentiation Potential ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue
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