scholarly journals High mobility group B proteins regulate mesoderm formation and dorsoventral patterning during zebrafish and Xenopus early development

2012 ◽  
Vol 129 (9-12) ◽  
pp. 263-274 ◽  
Author(s):  
Jian-Meng Cao ◽  
Shang-Qi Li ◽  
Hong-Wei Zhang ◽  
De-Li Shi
Keyword(s):  
Early Development ◽  
High Mobility ◽  
High Mobility Group ◽  
Dorsoventral Patterning ◽  
Mesoderm Formation ◽  
Group B

scholarly journals Mutagenesis of the HMGB (high-mobility group B) protein Cmb1 (cytosine-mismatch binding 1) of Schizosaccharomyces pombe: effects on recognition of DNA mismatches and damage

2003 ◽  
Vol 372 (2) ◽  
pp. 651-660 ◽  
Author(s):  
Christophe KUNZ ◽  
Karin ZURBRIGGEN ◽  
Oliver FLECK
Keyword(s):  
Amino Acids ◽  
Dna Binding ◽  
Schizosaccharomyces Pombe ◽  
Excision Repair ◽  
Specific Binding ◽  
High Mobility ◽  
High Mobility Group ◽  
Dna Mismatches ◽  
Terminal Amino ◽  
Group B

Cmb1 (cytosine-mismatch binding 1) is a high-mobility group (HMG) protein of Schizosaccharomyces pombe, which consists of 223 amino acids and has a single HMG domain at the C-terminal end. We have created several mutant and deletion forms of the Cmb1 protein and studied the effects on general DNA binding and specific binding to DNA mismatches and damaged DNA. Cmb1Δ41 (i.e. Cmb1 from which the 41 N-terminal amino acids have been deleted) bound specifically to cytosine-containing mismatches, to the cisplatin-induced intrastrand cross-links cis-GG and cis-AG and to an O6-methylguanine lesion. DNA binding was not affected when the 45 N-terminal amino acids were deleted, but was abolished in the absence of the 50 N-terminal amino acids, and was reduced when Cmb1 was truncated by between five and eleven C-terminal amino acids. Cmb1, both with and without the C-terminal truncations, retained its DNA binding affinity after heating at 95 °C. The cmb1 gene was induced when S. pombe cells were treated with cisplatin. Mitotic mutation rates were increased in a S. pombe cmb1 null mutant and in a cmb1-(1–212) mutant, which encodes a Cmb1 protein lacking the 11 C-terminal amino acids. We conclude that mutation avoidance by Cmb1 is distinct from Msh2-dependent mismatch repair, but related to nucleotide excision repair.

Molecular and functional characterization of single-box high-mobility group B (HMGB) chromosomal protein from Aedes aegypti

Gene ◽  
2018 ◽  
Vol 671 ◽  
pp. 152-160
Author(s):  
Isabel Caetano de Abreu da Silva ◽  
Amanda Roberta Revoredo Vicentino ◽  
Renata Coutinho dos Santos ◽  
Rodrigo Nunes da Fonseca ◽  
Anderson de Mendonça Amarante ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Functional Characterization ◽  
High Mobility ◽  
High Mobility Group ◽  
Chromosomal Protein ◽  
Group B

Involvement of High Mobility Group B Proteins in Cisplatin-Induced Cytotoxicity in Squamous Cell Carcinoma of Skin

2009 ◽  
Vol 28 (7) ◽  
pp. 311-318 ◽  
Author(s):  
Ashok Sharma ◽  
Allam Ramanjaneyulu ◽  
Ruma Ray ◽  
Moganty R. Rajeswari
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
High Mobility ◽  
High Mobility Group ◽  
Group B

scholarly journals Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility

2012 ◽  
Vol 41 (1) ◽  
pp. 167-181 ◽  
Author(s):  
Micah J. McCauley ◽  
Emily M. Rueter ◽  
Ioulia Rouzina ◽  
L. James Maher ◽  
Mark C. Williams
Keyword(s):  
Single Molecule ◽  
High Mobility ◽  
High Mobility Group ◽  
Microscopic Mechanism ◽  
Dna Flexibility ◽  
Group B

scholarly journals High Mobility Group B-1 (HMGB-1) Promotes Apoptosis of Macrophage-Derived Foam Cells by Inducing Endoplasmic Reticulum Stress

2018 ◽  
Vol 48 (3) ◽  
pp. 1019-1029 ◽  
Author(s):  
Han Wu ◽  
Zheng Chen ◽  
Jian-Zhou Chen ◽  
Li-Gang Pei ◽  
Jun Xie ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Lipid Accumulation ◽  
High Mobility ◽  
Foam Cells ◽  
High Mobility Group ◽  
Foam Cell Formation ◽  
Dependent Manner ◽  
Group B

Background/Aims: High mobility group B-1 (HMGB-1)-induced endoplasmic reticulum stress (ERS) has been implicated in inflammation and dendritic cell maturation. C/EBP-homologous protein (CHOP) is a vital component of ERS and apoptosis and plays a critical role in atherosclerosis. However, only a little information is available about the role of HMGB-1 in foam cell formation. Thus, the role of HMGB-1-induced ERS/CHOP pathway in apoptosis and formation of macrophage-derived foam cells is investigated. Methods: RAW264.7 cells were treated with oxidized low-density lipoprotein (oxLDL) in the absence and/or presence of HMGB-1, N-acetylcysteine (NAC, an antioxidant), glycyrrhizin (Gly, an HMGB-1 inhibitor), tunicamycin (TM, an ERS inducer), and 4-phenylbutyrate (4-PBA, an ERS inhibitor). Reactive oxygen species (ROS) production was examined by dihydroethidium (DHE) staining. Oil Red O staining, intracellular total cholesterol assay, and Dil-oxLDL uptake assay evaluated the accumulation of lipids in macrophages. Cell apoptosis was measured by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Western blot detected the expression of HMGB-1/ERS/CHOP pathway. Results: oxLDL induced HMGB-1 translocation and secretion in a dose- and time-dependent manner, which was inhibited by NAC. oxLDL-induced lipid accumulation in macrophages was promoted synergistically by HMGB-1 that was attenuated by Gly. Moreover, TM synergized with oxLDL induced lipid accumulation and apoptosis of macrophages; however, 4-PBA alleviated the oxLDL-induced apoptotic foam cells. Additionally, the inhibition of ERS with 4-PBA suppressed the expression of HMGB-1-induced CHOP. Conclusions: OxLDL triggered HMGB-1 secretion in macrophages via oxidative stress. Furthermore, HMGB-1 promoted the formation and apoptosis of macrophage-derived foam cells via activation of ERS/CHOP pathway.

scholarly journals Single-molecule studies of high-mobility group B architectural DNA bending proteins

2016 ◽  
Vol 9 (1) ◽  
pp. 17-40 ◽  
Author(s):  
Divakaran Murugesapillai ◽  
Micah J. McCauley ◽  
L. James Maher ◽  
Mark C. Williams
Keyword(s):  
Single Molecule ◽  
Dna Bending ◽  
High Mobility ◽  
High Mobility Group ◽  
Single Molecule Studies ◽  
Group B
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