scholarly journals 05-P003 A novel role for p63 in establishing tissue-specific chromatin organization and high-order chromatin remodelling in epidermal keratinocytes during skin development

2009 ◽  
Vol 126 ◽  
pp. S113-S114
Author(s):  
Michael Fessing ◽  
Andrei Mardaryev ◽  
Michal Gdula ◽  
Andrey Sharov ◽  
Tatyana Sharova ◽  
...  
Keyword(s):  
High Order ◽  
Chromatin Remodelling ◽  
Chromatin Organization ◽  
Epidermal Keratinocytes ◽  
Tissue Specific ◽  
Skin Development ◽  
High Order Chromatin

scholarly journals Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells

2022 ◽  
Vol 79 (1) ◽  
Author(s):  
Geng G. Tian ◽  
Xinyan Zhao ◽  
Changliang Hou ◽  
Wenhai Xie ◽  
Xiaoyong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chromatin Structure ◽  
Three Dimensional ◽  
High Order ◽  
Chromatin Organization ◽  
Germline Stem Cells ◽  
High Order Chromatin Structure ◽  
Female Germline ◽  
High Order Chromatin

AbstractThe three-dimensional configuration of the genome ensures cell type-specific gene expression profiles by placing genes and regulatory elements in close spatial proximity. Here, we used in situ high-throughput chromosome conformation (in situ Hi-C), RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to characterize the high-order chromatin structure signature of female germline stem cells (FGSCs) and identify its regulating key factor based on the data-driven of multiple omics data. By comparison with pluripotent stem cells (PSCs), adult stem cells (ASCs), and somatic cells at three major levels of chromatin architecture, A/B compartments, topologically associating domains, and chromatin loops, the chromatin architecture of FGSCs was most similar to that of other ASCs and largely different from that of PSCs and somatic cells. After integrative analysis of the three-dimensional chromatin structure, active compartment-associating loops (aCALs) were identified as a signature of high-order chromatin organization in FGSCs, which revealed that CCCTC-binding factor was a major factor to maintain the properties of FGSCs through regulation of aCALs. We found FGSCs belong to ASCs at chromatin structure level and characterized aCALs as the high-order chromatin structure signature of FGSCs. Furthermore, CTCF was identified to play a key role in regulating aCALS to maintain the biological functions of FGSCs. These data provide a valuable resource for future studies of the features of chromatin organization in mammalian stem cells and further understanding of the fundamental characteristics of FGSCs.

scholarly journals Sparse conserved under-methylated CpGs are associated with high-order chromatin structure

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Xueqiu Lin ◽  
Jianzhong Su ◽  
Kaifu Chen ◽  
Benjamin Rodriguez ◽  
Wei Li
Keyword(s):  
Chromatin Structure ◽  
High Order ◽  
High Order Chromatin Structure ◽  
High Order Chromatin

Chromatin remodelling and nuclear reprogramming at the onset of embryonic development in mammals

1998 ◽  
Vol 10 (8) ◽  
pp. 573 ◽  
Author(s):  
Jean-Paul Renard
Keyword(s):  
Normal Development ◽  
Blastocyst Stage ◽  
Chromatin Remodelling ◽  
Chromatin Organization ◽  
Nuclear Reprogramming ◽  
Cell Stage ◽  
Donor Nucleus ◽  
The One ◽  
Kinetics Of ◽  
Determining Factor

Two main strategies are used to produce cloned mammals. The first involves the condensation of donor chromatin into chromosomes directly exposed to the recipient cytoplasm, whereas the second leaves the donor nucleus in interphase until the time of the first mitosis. Both strategies, which induce marked changes in chromatin organization, allow full reprogrammation of somatic-differentiated fetal and adult cells. This paper reviews some of the recent data that contribute to our understanding of chromatin remodelling at the onset of normal development, as well as after the introduction of a foreign nucleus into a recipient enucleated oocyte. These data indicate that the coordinated changes in chromatin organization that take place up until the first cellular differentiations at the blastocyst stage are determinants for successful cloning. Although some degree of synchronization between the cell cycle stages of donor and recipient cells is necessary for correct remodelling of a transferred nucleus, the kinetics of remodelling events occurring during the one-cell stage appears to be the determining factor for the normal onset of gene expression.

DNA structure, nucleosome placement and chromatin remodelling: a perspective

2012 ◽  
Vol 40 (2) ◽  
pp. 335-340 ◽  
Author(s):  
Andrew A. Travers ◽  
Cédric Vaillant ◽  
Alain Arneodo ◽  
Georgi Muskhelishvili
Keyword(s):  
Dna Sequence ◽  
Thermodynamic Stability ◽  
Budding Yeast ◽  
Nucleosome Positioning ◽  
Chromatin Remodelling ◽  
Chromatin Organization ◽  
Eukaryotic Genome ◽  
Major Question ◽  
Chromatin Biology

A major question in chromatin biology is to what extent the sequence of DNA directly determines the genetic and chromatin organization of a eukaryotic genome? We consider two aspects to this question: the DNA sequence-specified positioning of nucleosomes and the determination of NDRs (nucleosome-depleted regions) or barriers. We argue that, in budding yeast, while DNA sequence-specified nucleosome positioning may contribute to positions flanking the regions lacking nucleosomes, DNA thermodynamic stability is a major component determinant of the genetic organization of this organism.

scholarly journals High-order chromatin structure and the epigenome in SAHFs

Nucleus ◽  
2013 ◽  
Vol 4 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Tamir Chandra ◽  
Masashi Narita
Keyword(s):  
Chromatin Structure ◽  
High Order ◽  
High Order Chromatin Structure ◽  
High Order Chromatin

Effects of fixatives on high order chromatin structure: a calorimetric study

1992 ◽  
Vol 206 ◽  
pp. 175-179 ◽  
Author(s):  
L. Vergani ◽  
G. Mascetti ◽  
P. Gavazzo ◽  
C. Nicolini
Keyword(s):  
Chromatin Structure ◽  
High Order ◽  
Calorimetric Study ◽  
High Order Chromatin Structure ◽  
High Order Chromatin

Mouse keratinocytes immortalized with large T antigen acquire alpha3beta1 integrin-dependent secretion of MMP-9/gelatinase B

2000 ◽  
Vol 113 (16) ◽  
pp. 2909-2921 ◽  
Author(s):  
C.M. DiPersio ◽  
M. Shao ◽  
L. Di Costanzo ◽  
J.A. Kreidberg ◽  
R.O. Hynes
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cell Invasion ◽  
T Antigen ◽  
Epidermal Keratinocytes ◽  
Large T Antigen ◽  
Gelatinase B ◽  
Skin Development ◽  
Mouse Keratinocytes ◽  
Dependent Pathway

Remodeling of the extracellular matrix during tissue development, wound repair and tumor cell invasion depends on the coordinated regulation of cell adhesion receptors, matrix proteins and enzymes that proteolyse the extracellular matrix. Integrin alpha3beta1 is a major receptor on epidermal keratinocytes for laminin-5 in the cutaneous basement membrane and is required for normal basement membrane organization during skin development. alpha3beta1 is also expressed at high levels in the majority of adherent transformed cells and in most tumors, and it could have similar roles in extracellular matrix remodeling during tumorigenesis and cell invasion. In the present study, we show that alpha3beta1 expression is required in immortalized mouse keratinocytes (MK) for the production of the matrix metalloproteinase MMP-9/gelatinase B, an MMP that is coexpressed with alpha3beta1 in epithelial cell carcinomas and during wound healing, and contributes to the invasive potential of some tumor cells. MMP-9 was expressed in MK cells derived from wild-type mice, but not in MK cells derived from alpha3-null mice. Reconstitution of alpha3beta1 expression in alpha3-null MK cells through transfection with the alpha3 subunit restored MMP-9 secretion, indicating an alpha3beta1-dependent pathway for MMP-9 production. alpha3beta1-dependent expression of MMP-9 was associated with the immortalized phenotype, since nonimmortalized, primary keratinocytes required soluble growth factors, but not alpha3beta1, for efficient expression of MMP-9. Our results suggest that an alpha3beta1-independent pathway(s) for MMP-9 production is suppressed in keratinocytes immortalized with large T antigen, and that an alpha3beta1-dependent pathway is required for sustained production of MMP-9 in the absence of other pathways.

scholarly journals Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development

1997 ◽  
Vol 94 (14) ◽  
pp. 7400-7405 ◽  
Author(s):  
M. Tarutani ◽  
S. Itami ◽  
M. Okabe ◽  
M. Ikawa ◽  
T. Tezuka ◽  
...  
Keyword(s):  
Tissue Specific ◽  
Skin Development
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