scholarly journals Involvement of Mos–MEK–MAPK pathway in cytostatic factor (CSF) arrest in eggs of the parthenogenetic insect, Athalia rosae

2008 ◽  
Vol 125 (11-12) ◽  
pp. 996-1008 ◽  
Author(s):  
Daisuke S. Yamamoto ◽  
Kazunori Tachibana ◽  
Megumi Sumitani ◽  
Jae Min Lee ◽  
Masatsugu Hatakeyama
Keyword(s):  
Mapk Pathway ◽  
Athalia Rosae ◽  
Cytostatic Factor

scholarly journals Regulation of Raf-1-dependent signaling during early Xenopus development.

1995 ◽  
Vol 15 (12) ◽  
pp. 6686-6693 ◽  
Author(s):  
A M MacNicol ◽  
A J Muslin ◽  
E L Howard ◽  
A Kikuchi ◽  
M C MacNicol ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Mapk Pathway ◽  
Mapk Signaling ◽  
Embryonic Cell ◽  
Cycle Progression ◽  
Mapk Activity ◽  
Cytostatic Factor ◽  
Differentiation And Proliferation

The Raf-1 gene product is activated in response to cellular stimulation by a variety of growth factors and hormones. Raf-1 activity has been implicated in both cellular differentiation and proliferation. We have examined the regulation of the Raf-1/MEK/MAP kinase (MAPK) pathway during embryonic development in the frog Xenopus laevis. We report that Raf-1, MEK, and MAPK activities are turned off following fertilization and remain undetectable up until blastula stages (stage 8), some 4 h later. Tight regulation of the Raf-1/MEK/MAPK pathway following fertilization is crucial for embryonic cell cycle progression. Inappropriate reactivation of MAPK activity by microinjection of oncogenic Raf-1 RNA results in metaphase cell cycle arrest and, consequently, embryonic lethality. Our findings demonstrate an absolute requirement, in vivo, for inactivation of the MAPK signaling pathway to allow normal cell cycle progression during the period of synchronous cell divisions which occur following fertilization. Further, we show that cytostatic factor effects are mediated through MEK and MAPK.

scholarly journals Meiotic spindle stability depends on MAPK-interacting and spindle-stabilizing protein (MISS), a new MAPK substrate

2002 ◽  
Vol 157 (4) ◽  
pp. 603-613 ◽  
Author(s):  
Christophe Lefebvre ◽  
M. Emilie Terret ◽  
Alexandre Djiane ◽  
Pascale Rassinier ◽  
Bernard Maro ◽  
...  
Keyword(s):  
Antisense Rna ◽  
Mapk Pathway ◽  
Meiotic Spindle ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein ◽  
Cytostatic Factor ◽  
Morpholino Oligonucleotides ◽  
Spindle Stability ◽  
Two Hybrid ◽  
Segregation Of Chromosomes

Vertebrate oocytes arrest in the second metaphase of meiosis (metaphase II [MII]) by an activity called cytostatic factor (CSF), with aligned chromosomes and stable spindles. Segregation of chromosomes occurs after fertilization. The Mos/…/MAPK (mitogen-activated protein kinases) pathway mediates this MII arrest. Using a two-hybrid screen, we identified a new MAPK partner from a mouse oocyte cDNA library. This protein is unstable during the first meiotic division and accumulates only in MII, where it localizes to the spindle. It is a substrate of the Mos/…/MAPK pathway. The depletion of endogenous RNA coding for this protein by three different means (antisense RNA, double-stranded [ds] RNA, or morpholino oligonucleotides) induces severe spindle defects specific to MII oocytes. Overexpressing the protein from an RNA not targeted by the morpholino rescues spindle destabilization. However, dsRNA has no effect on the first two mitotic divisions. We therefore have discovered a new MAPK substrate involved in maintaining spindle integrity during the CSF arrest of mouse oocytes, called MISS (for MAP kinase–interacting and spindle-stabilizing protein).

scholarly journals p90Rsk is not involved in cytostatic factor arrest in mouse oocytes

2005 ◽  
Vol 169 (2) ◽  
pp. 227-231 ◽  
Author(s):  
Julien Dumont ◽  
Muriel Umbhauer ◽  
Pascale Rassinier ◽  
André Hanauer ◽  
Marie-Hélène Verlhac
Keyword(s):  
Mapk Pathway ◽  
Xenopus Laevis Oocytes ◽  
S6 Kinase ◽  
Mouse Oocytes ◽  
Cycle Arrest ◽  
A Cell ◽  
Ribosomal S6 Kinase ◽  
Cytostatic Factor ◽  
Morpholino Oligonucleotides ◽  
Mutant Forms

Vertebrate oocytes arrest in metaphase of the second meiotic division (MII), where they maintain a high cdc2/cyclin B activity and a stable, bipolar spindle because of cytostatic factor (CSF) activity. The Mos–MAPK pathway is essential for establishing CSF. Indeed, oocytes from the mos−/− strain do not arrest in MII and activate without fertilization, as do Xenopus laevis oocytes injected with morpholino oligonucleotides directed against Mos. In Xenopus oocytes, p90Rsk (ribosomal S6 kinase), a MAPK substrate, is the main mediator of CSF activity. We show here that this is not the case in mouse oocytes. The injection of constitutively active mutant forms of Rsk1 and Rsk2 does not induce a cell cycle arrest in two-cell mouse embryos. Moreover, these two mutant forms do not restore MII arrest after their injection into mos−/− oocytes. Eventually, oocytes from the triple Rsk (1, 2, 3) knockout present a normal CSF arrest. We demonstrate that p90Rsk is not involved in the MII arrest of mouse oocytes.

The Mos/MAPK pathway is involved in metaphase II arrest as a cytostatic factor but is neither necessary nor sufficient for initiating oocyte maturation in goldfish

2000 ◽  
Vol 210 (8-9) ◽  
pp. 416-425 ◽  
Author(s):  
Hiroko Kajiura-Kobayashi ◽  
Noriyuki Yoshida ◽  
Noriyuki Sagata ◽  
M. Yamashita ◽  
Yoshitaka Nagahama
Keyword(s):  
Oocyte Maturation ◽  
Mapk Pathway ◽  
Cytostatic Factor

P102 ROLE OF ENDOGENOUS MAPK PATHWAY SUPPRESSOR ON IBD

2018 ◽  
Vol 154 (1) ◽  
pp. S52
Author(s):  
Sharad Khare ◽  
Qiong Zhang
Keyword(s):  
Mapk Pathway

Induction of aldo-keto reductase 1C1–3 (AKR1C1–3) by thyroid hormone requires the TRβ and activation of the MAPK pathway

2007 ◽  
Vol 115 (S 1) ◽  
Author(s):  
LC Moeller ◽  
NE Haselhorst ◽  
AM Dumitrescu ◽  
S Refetoff ◽  
K Mann ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Mapk Pathway

Role of the p38 MAPK Pathway in Induction of iNOS Expression in Human Leukocytes

2008 ◽  
Vol 56 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Ewa Jablonska ◽  
Wioletta Ratajczak ◽  
Jakub Jablonski
Keyword(s):  
P38 Mapk ◽  
Mapk Pathway ◽  
Inos Expression ◽  
Human Leukocytes ◽  
P38 Mapk Pathway
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