scholarly journals In Drosophila melanogaster, the Rolling pebbles isoform 6 (Rols6) is essential for proper Malpighian tubule morphology

2005 ◽  
Vol 122 (11) ◽  
pp. 1206-1217 ◽  
Author(s):  
Michael Pütz ◽  
Dörthe Andrea Kesper ◽  
Detlev Buttgereit ◽  
Renate Renkawitz-Pohl
Keyword(s):  
Drosophila Melanogaster ◽  
Malpighian Tubule

scholarly journals The chloride channel Bestrophin‐1 acts in the Malpighian tubule and hindgut of Drosophila melanogaster to maintain salt and water balance

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Aylin Rodan ◽  
John Pleinis ◽  
Sima Jonusaite ◽  
Jacob Hudac ◽  
Austin Goodwin ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Water Balance ◽  
Chloride Channel ◽  
Malpighian Tubule ◽  
Salt And Water Balance

Molecular characterization of the Drosophila melanogaster urate oxidase gene, an ecdysone-repressible gene expressed only in the malpighian tubules

1990 ◽  
Vol 10 (10) ◽  
pp. 5114-5127
Author(s):  
L L Wallrath ◽  
J B Burnett ◽  
T B Friedman
Keyword(s):  
Drosophila Melanogaster ◽  
Amino Acid ◽  
Regulatory Element ◽  
Malpighian Tubule ◽  
Amino Acid Sequences ◽  
Malpighian Tubules ◽  
P Element ◽  
Urate Oxidase ◽  
Base Pairs ◽  
Oxidase Gene

The urate oxidase (UO) gene of Drosophila melanogaster is expressed during the third-instar larval and adult stages, exclusively within a subset of cells of the Malpighian tubules. The UO gene contains a 69-base-pair intron and encodes mature mRNAs of 1,224, 1,227, and 1,244 nucleotides, depending on the site of 3' endonucleolytic cleavage prior to polyadenylation. A direct repeat, 5'-AAGTGAGAGTGAT-3', is the proposed cis-regulatory element involved in 20-hydroxyecdysone repression of the UO gene. The deduced amino acid sequences of UO of D. melanogaster, rat, mouse, and pig and uricase II of soybean show 32 to 38% identity, with 22% of amino acid residues identical in all species. With use of P-element-mediated germ line transformation, 826 base pairs 5' and approximately 1,200 base pairs 3' of the D. melanogaster UO transcribed region contain all of the cis elements allowing for appropriate temporal regulation and Malpighian tubule-specific expression of the UO gene.

scholarly journals Zinc storage granules in the Malpighian tubules of Drosophila melanogaster

2017 ◽  
Author(s):  
Carlos Tejeda-Guzmán ◽  
Abraham Rosas-Arellano ◽  
Thomas Kroll ◽  
Samuel M. Webb ◽  
Martha Barajas-Aceves ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Malpighian Tubule ◽  
Adaptor Protein ◽  
Mossy Fibers ◽  
Membrane Transporters ◽  
Malpighian Tubules ◽  
Pancreatic Cells ◽  
Storage Granules ◽  
Hermansky Pudlak Syndrome ◽  
Zinc Storage

ABSTRACTMembrane transporters and sequestration mechanisms concentrate metal ions differentially into discrete subcellular microenvironments for usage in protein cofactors, signaling, storage, or excretion. Here we identify zinc storage granules as the insect’s major zinc reservoir in primary Malpighian tubule epithelial cells of Drosophila melanogaster. The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet (ABCG2-like) and ZnT35C transporters is required for zinc storage granule biogenesis. Due to similar lysosome related organelle defects, patients with Hermansky-Pudlak syndrome may lack zinc granules in beta pancreatic cells, intestinal paneth cells and presynaptic vesicles of hippocampal mossy fibers.

Characterization of theDrosophila melanogasteralkali-metal/proton exchanger (NHE) gene family

2001 ◽  
Vol 204 (21) ◽  
pp. 3703-3716 ◽  
Author(s):  
Maria E. Giannakou ◽  
Julian A. T. Dow
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Sequence ◽  
Malpighian Tubule ◽  
Malpighian Tubules ◽  
Na Channel ◽  
Rt Pcr ◽  
Multiple Transcripts ◽  
Homology Searching ◽  
Amiloride Derivatives

SUMMARYThe NHE family of Na+/H+ exchangers is believed to play an essential role in animals, but may play an additional, specialised epithelial role in insects. The pharmacological sensitivity of the Drosophila melanogaster Malpighian tubule to a range of amiloride derivatives was shown to be consistent with an effect on an exchanger, rather than a Na+ channel. Consistent with this, no degenerin/epithelial Na+ channel (ENaC) genes could be detected in Malpighian tubules by reverse transcriptase/polymerase chain reaction (RT-PCR). Using a low-stringency homology searching, three members of the NHE family were identified in the genomic sequence of Drosophila melanogaster, although only two genes were represented as expressed sequence tags. All three genes (DmNHE1 at cytological position 21B1, DmNHE2 at 39B1 and DmNHE3 at 27A1) were found by RT-PCR to be widely expressed, and one (DmNHE2) was shown to have multiple transcripts. The putative translations of the three genes mark them as distantly related members of the family, inviting the possibility that they may serve distinct roles in insects.

scholarly journals Identification of Genes Controlling Malpighian Tubule and Other Epithelial Morphogenesis in Drosophila melanogaster

Genetics ◽  
1999 ◽  
Vol 151 (2) ◽  
pp. 685-695
Author(s):  
Xuejun Liu ◽  
István Kiss ◽  
Judith A Lengyel
Keyword(s):  
Drosophila Melanogaster ◽  
Uric Acid ◽  
Malpighian Tubule ◽  
Model System ◽  
Epithelial Morphogenesis ◽  
Phenotypic Analysis ◽  
Genetic Pathway ◽  
Novel Gene ◽  
Genetic Mechanisms

Abstract The Drosophila Malpighian tubule is a model system for studying genetic mechanisms that control epithelial morphogenesis. From a screen of 1800 second chromosome lethal lines, by observing uric acid deposits in unfixed inviable embryos, we identified five previously described genes (barr, fas, flb, raw, and thr) and one novel gene, walrus (wal), that affect Malpighian tubule morphogenesis. Phenotypic analysis of these mutant embryos allows us to place these genes, along with other previously described genes, into a genetic pathway that controls Malpighian tubule development. Specifically, wal affects evagination of the Malpighian tubule buds, fas and thr affect bud extension, and barr, flb, raw, and thr affect tubule elongation. In addition, these genes were found to have different effects on development of other epithelial structures, such as foregut and hindgut morphogenesis. Finally, from the same screen, we identified a second novel gene, drumstick, that affects only foregut and hindgut morphogenesis.

Developmental expression and biophysical characterization of a Drosophila melanogaster aquaporin

2005 ◽  
Vol 289 (2) ◽  
pp. C397-C407 ◽  
Author(s):  
Nancy Kaufmann ◽  
John C. Mathai ◽  
Warren G. Hill ◽  
Julian A. T. Dow ◽  
Mark L. Zeidel ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Water Transport ◽  
Molecular Mechanisms ◽  
Fluid Transport ◽  
Genetic Manipulation ◽  
Sequence Similarity ◽  
Malpighian Tubule ◽  
Developmental Expression ◽  
Drosophila Genome ◽  
Secretory Vesicles

Aquaporins (AQPs) accelerate the movement of water and other solutes across biological membranes, yet the molecular mechanisms of each AQP's transport function and the diverse physiological roles played by AQP family members are still being defined. We therefore have characterized an AQP in a model organism, Drosophila melanogaster, which is amenable to genetic manipulation and developmental analysis. To study the mechanism of Drosophila Malpighian tubule (MT)-facilitated water transport, we identified seven putative AQPs in the Drosophila genome and found that one of these, previously named DRIP, has the greatest sequence similarity to those vertebrate AQPs that exhibit the highest rates of water transport. In situ mRNA analyses showed that DRIP is expressed in both embryonic and adult MTs, as well as in other tissues in which fluid transport is essential. In addition, the pattern of DRIP expression was dynamic. To define DRIP-mediated water transport, the protein was expressed in Xenopus oocytes and in yeast secretory vesicles, and we found that significantly elevated rates of water transport correlated with DRIP expression. Moreover, the activation energy required for water transport in DRIP-expressing secretory vesicles was 4.9 kcal/mol. This low value is characteristic of AQP-mediated water transport, whereas the value in control vesicles was 16.4 kcal/mol. In contrast, glycerol, urea, ammonia, and proton transport were unaffected by DRIP expression, suggesting that DRIP is a highly selective water-specific channel. This result is consistent with the homology between DRIP and mammalian water-specific AQPs. Together, these data establish Drosophila as a new model system with which to investigate AQP function.

Analysis of malpighian tubule membrane proteins from Drosophila melanogaster

1984 ◽  
Vol 14 (6) ◽  
pp. 729-737
Author(s):  
David T. Sullivan ◽  
Bernadette K. Lukacs ◽  
Beth F. Mitchell
Keyword(s):  
Drosophila Melanogaster ◽  
Membrane Proteins ◽  
Malpighian Tubule
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