Analysis of p53 binding to DNA by fluorescence imaging microscopy

Micron ◽  
2012 ◽  
Vol 43 (9) ◽  
pp. 996-1000 ◽  
Author(s):  
Hwee Jing Ong ◽  
Jia Wei Siau ◽  
Jing Bo Zhang ◽  
Minghui Hong ◽  
Horst Flotow ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Imaging Microscopy

scholarly journals Simultaneous Measurement of Water Volume and pH in Single Cells Using BCECF and Fluorescence Imaging Microscopy

2006 ◽  
Vol 90 (2) ◽  
pp. 608-618 ◽  
Author(s):  
Francisco J. Alvarez-Leefmans ◽  
José J. Herrera-Pérez ◽  
Martín S. Márquez ◽  
Víctor M. Blanco
Keyword(s):  
Fluorescence Imaging ◽  
Simultaneous Measurement ◽  
Single Cells ◽  
Water Volume ◽  
Fluorescence Imaging Microscopy

scholarly journals Investigations of RhB Langmuir monolayer by Fluorescence Imaging Microscopy

2010 ◽  
Vol 84 (6) ◽  
pp. 729-733 ◽  
Author(s):  
S. A. Hussain ◽  
S. Chakraborty ◽  
D. Bhattacharjee
Keyword(s):  
Fluorescence Imaging ◽  
Langmuir Monolayer ◽  
Fluorescence Imaging Microscopy

Multi photon excitation fluorescence imaging microscopy for the precise characterization of corrosion layers in silver-based artifacts

2013 ◽  
Vol 111 (1) ◽  
pp. 177-181 ◽  
Author(s):  
F. Faraldi ◽  
G. J. Tserevelakis ◽  
G. Filippidis ◽  
G. M. Ingo ◽  
C. Riccucci ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Photon Excitation ◽  
Precise Characterization ◽  
Fluorescence Imaging Microscopy

scholarly journals Signal to noise analysis of multiple color fluorescence imaging microscopy

Cytometry ◽  
1999 ◽  
Vol 35 (3) ◽  
pp. 214-226 ◽  
Author(s):  
Y. Garini ◽  
A. Gil ◽  
I. Bar-Am ◽  
D. Cabib ◽  
N. Katzir
Keyword(s):  
Fluorescence Imaging ◽  
Noise Analysis ◽  
Signal To Noise ◽  
Fluorescence Imaging Microscopy

scholarly journals Cellular uptake and intracellular localization of benzo(a)pyrene by digital fluorescence imaging microscopy.

1985 ◽  
Vol 100 (4) ◽  
pp. 1295-1308 ◽  
Author(s):  
A L Plant ◽  
D M Benson ◽  
L C Smith
Keyword(s):  
Fluorescence Imaging ◽  
Cellular Uptake ◽  
Intracellular Localization ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Intracellular Location ◽  
Fluorescence Imaging Microscopy ◽  
Digital Fluorescence Imaging

Uptake of benzo(a)pyrene by living cultured cells has been visualized in real time using digital fluorescence-imaging microscopy. Benzo(a)pyrene was noncovalently associated with lipoproteins, as a physiologic mode of presentation of the carcinogen to cells. When incubated with either human fibroblasts or murine P388D1 macrophages, benzo(a)pyrene uptake occurred in the absence of endocytosis, with a halftime of approximately 2 min, irrespective of the identity of the delivery vehicles, which were high density lipoproteins, low density lipoproteins, very low density lipoproteins, and 1-palmitoyl-2-oleoylphosphatidylcholine single-walled vesicles. Thus, cellular uptake of benzo(a)pyrene from these hydrophobic donors occurs by spontaneous transfer through the aqueous phase. Moreover, the rate constant for uptake, the extent of uptake, and the intracellular localization of benzo(a)pyrene were identical for both living and fixed cells. Similar rate constants for benzo(a)pyrene efflux from cells to extracellular lipoproteins suggests the involvement of the plasma membrane in the rate-limiting step. The intracellular location of benzo(a)pyrene at equilibrium was coincident with a fluorescent cholesterol analog, N-(7-nitrobenz-2-oxa-1,3-diazole)-23,24-dinor-5-cholen-22-amine-3 beta-ol. Benzo(a)pyrene did not accumulate in acidic compartments, based on acridine orange fluorescence, or in mitochondria, based on rhodamine-123 fluorescence. When the intracellular lipid volume of isolated mouse peritoneal macrophages was increased by prior incubation of these cells with either acetylated low density lipoproteins or with very low density lipoproteins from a hypertriglyceridemic individual, cellular accumulation of benzo(a)pyrene increased proportionately with increased [1-14C]oleate incorporation into cellular triglycerides and cholesteryl esters. Thus, benzo(a)pyrene uptake by cells is a simple partitioning phenomenon, controlled by the relative lipid volumes of extracellular donor lipoproteins and of cells, and does not involve lipoprotein endocytosis as an obligatory step.

Sign in / Sign up

Export Citation Format

Share Document