scholarly journals In vivo imaging of cell morphology and cellular processes in Caenorhabditis elegans, using non-linear phenomena

Micron ◽  
2009 ◽  
Vol 40 (8) ◽  
pp. 876-880 ◽  
Author(s):  
G. Filippidis ◽  
E.J. Gualda ◽  
M. Mari ◽  
K. Troulinaki ◽  
C. Fotakis ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Morphology ◽  
In Vivo Imaging ◽  
Cellular Processes ◽  
Non Linear

Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans

2007 ◽  
Vol 4 (9) ◽  
pp. 727-731 ◽  
Author(s):  
Nikos Chronis ◽  
Manuel Zimmer ◽  
Cornelia I Bargmann
Keyword(s):  
Caenorhabditis Elegans ◽  
In Vivo Imaging ◽  
Behavioral Activity

Multidimensional custom-made non-linear microscope: from ex-vivo to in-vivo imaging

2008 ◽  
Vol 92 (3) ◽  
pp. 359-365 ◽  
Author(s):  
R. Cicchi ◽  
L. Sacconi ◽  
A. Jasaitis ◽  
R.P. O’Connor ◽  
D. Massi ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Ex Vivo ◽  
Custom Made ◽  
Non Linear

scholarly journals Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo

2021 ◽  
Author(s):  
Takayuki Torisawa ◽  
Akatsuki Kimura
Keyword(s):  
Caenorhabditis Elegans ◽  
Cytoplasmic Dynein ◽  
Early Embryo ◽  
Regulatory Proteins ◽  
Cellular Regulation ◽  
Cellular Processes ◽  
Nuclear Envelope Breakdown ◽  
Spatiotemporal Localization ◽  
Region Ii

Cytoplasmic dynein is responsible for various cellular processes during the cell cycle. The mechanism by which its activity is regulated spatially and temporarily inside the cell remains elusive. There are various regulatory proteins of dynein, including dynactin, NDEL1/NUD-2, and LIS1. Characterizing the spatiotemporal localization of regulatory proteins in vivo will aid understanding of the cellular regulation of dynein. Here, we focused on spindle formation in the Caenorhabditis elegans early embryo, wherein dynein and its regulatory proteins translocated from the cytoplasm to the spindle region upon nuclear envelope breakdown (NEBD). We found that (i) a limited set of dynein regulatory proteins accumulated in the spindle region, (ii) the spatial localization patterns were distinct among the regulators, and (iii) the regulatory proteins did not accumulate in the spindle region simultaneously but sequentially. Furthermore, the accumulation of NUD-2 was unique among the regulators. NUD-2 started to accumulate before NEBD (pre-NEBD accumulation), and exhibited the highest enrichment compared to the cytoplasmic concentration. Using a protein injection approach, we revealed that the C-terminal helix of NUD-2 was responsible for pre-NEBD accumulation. These findings suggest a fine temporal control of the subcellular localization of regulatory proteins.

Sign in / Sign up

Export Citation Format

Share Document