A validated HPLC-DAD method for simultaneous determination of major bioactive constituents of Antidiabetic herbal extracts; application to a newly Co-formulated nutraceutical product

2020 ◽  
Vol 159 ◽  
pp. 105472
Author(s):  
Hoda M. Marzouk ◽  
Mahmoud H. Teaima ◽  
Ahmed M. El-Desoky ◽  
Shanaz O. Mohamed
Keyword(s):  
Simultaneous Determination ◽  
Herbal Extracts ◽  
Bioactive Constituents

Simultaneous Determination of 12 Bioactive Constituents in Bupi Yiqi No. 1 Recipe by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry

2020 ◽  
Vol 103 (1) ◽  
pp. 117-123
Author(s):  
Qingling Xie ◽  
Bin Li ◽  
Feibing Huang ◽  
Mengru Cao ◽  
Hanwen Yuan ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Multiple Reaction Monitoring ◽  
Cancer Recurrence ◽  
Correlation Coefficients ◽  
Bioactive Constituents ◽  
Column Temperature ◽  
Ginsenoside Re ◽  
Esi Ms ◽  
Stability And Accuracy

Abstract Background: Bupi Yiqi No. 1 Recipe (BPYQ), a traditional Chinese medicine formula, has been widely used as a treatment of multidrug resistance during chemotherapy in colorectal cancer and for the prevention of gastrointestinal cancer recurrence for decades. Objective: In this study, a rapid, reliable, and accurate ultrahigh-performance LC (UHPLC) coupled with electrospray ionization (ESI)–tandem MS (MS/MS) method was developed for the simultaneous determination of 12 major bioactive components, including protocatechuic acid, astilbin, rutin, calycosin-7-O-β-D-glucoside, ginsenoside Re, ononin, isoliquiritigenin, calycosin, apigenin, ginsenoside Rb1, formononetin, and glycyrrhizic acid in BPYQ. Methods: The chromatographic separation of the analytes was achieved on a Poroshell 120 SB-Aq column (50 × 2.1 mm, 1.7 µm) with a mobile phase of acetonitrile and 0.1% (v/v) formic acid aqueous solution. The flow rate and column temperature were set at 0.4 mL/min and 30°C, respectively. Mass spectrometric detection of the analyses was performed on multiple reaction monitoring mode in positive and negative ESI mode. Results: The established UHPLC–ESI–MS/MS method was validated in terms of the linearity, precision, repeatability, stability, and accuracy. All calibration curves of the 12 compounds showed good linearity, with correlation coefficients (r) greater than 0.9980 within the test ranges. The LODs and LOQs for the 12 compounds were in the ranges of 0.08–1.32 and 0.27–5.28 ng/mL, respectively. The average recoveries of all the standard compounds were between 98.4 and 102.9%, and their relative SD values ranged from 1.24 to 3.78%. Conclusions: The proposed method can provide a meaningful basis for the QC of BPYQ. Highlights: The established UHPLC-ESI-MS/MS method was demonstrated to be a powerful tool for quantifying the 12 compounds in BPYQ.

scholarly journals Analysis of Flavonoids and Iridoids in Vitex Negundo by HPLC-PDA and Method Validation

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800
Author(s):  
Somendu K. Roy ◽  
Khemraj Bairwa ◽  
Jagdeep Grover ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Method Validation ◽  
Hplc Method ◽  
Vitex Negundo ◽  
Good Recovery ◽  
Bioactive Constituents ◽  
Ich Guidelines ◽  
Validation Parameters

The leaves of Vitex negundo have been reported to contain various bioactive constituents including iridoids and flavonoids. This is the first report on the simultaneous determination of iridoids and flavonoids by HPLC in three different samples of V. negundo leaves collected from three regions of India. Separation of iridoids and flavonoids was accomplished by HPLC and further elaborated for their quantification in V. negundo leaves using a C-18 column with detection at 254 and 330 nm, respectively. The developed HPLC method showed good linearity (r2≥0.999), high precision (RSD<5%) and a good recovery (99.3–103.0%) of the compounds. All the validation parameters of the developed HPLC were found to be within the permissible limits according to the ICH guidelines. The developed method was robust, accurate and reliable for the quality control of V. negundo leaves.

scholarly journals Quality Control of the Fuzi Lizhong Pill Through Simultaneous Determination of 16 Major Bioactive Constituents by RRLC–MS-MS

2018 ◽  
Vol 56 (6) ◽  
pp. 541-554 ◽  
Author(s):  
Yun-zhuo Dong ◽  
Zhen-li Liu ◽  
Yuan-yan Liu ◽  
Zhi-qian Song ◽  
Na Guo ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Bioactive Constituents
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