Development of fluorescent pH sensors based on a sol-gel matrix for acidic and neutral pH ranges in a microtiter plate

2019 ◽  
Vol 147 ◽  
pp. 286-295 ◽  
Author(s):  
Hong Dinh Duong ◽  
Younsook Shin ◽  
Jong Il Rhee
1991 ◽  
Vol 27 (17) ◽  
pp. 1560 ◽  
Author(s):  
J.Y. Ding ◽  
M.R. Shahriari ◽  
G.H. Sigel
Keyword(s):  
Sol Gel ◽  

2021 ◽  
Vol 340 ◽  
pp. 129853
Author(s):  
Fei Lu ◽  
Ruishu Wright ◽  
Ping Lu ◽  
Patricia C. Cvetic ◽  
Paul R. Ohodnicki
Keyword(s):  
Sol Gel ◽  

2017 ◽  
Vol 64 (9) ◽  
pp. 3971-3975 ◽  
Author(s):  
Sankar Prasad Bag ◽  
Bih-Show Lou ◽  
Jim-Long Her ◽  
See-Tong Pang ◽  
Tung-Ming Pan

2005 ◽  
Vol 77 (3) ◽  
pp. 848-853 ◽  
Author(s):  
Stephanie M. Marxer ◽  
Mark H. Schoenfisch
Keyword(s):  
Sol Gel ◽  

1992 ◽  
Vol 13 (6) ◽  
pp. 293-298 ◽  
Author(s):  
C. Rottman ◽  
M. Ottolenghi ◽  
R. Zusman ◽  
O. Lev ◽  
M. Smith ◽  
...  
Keyword(s):  
Sol Gel ◽  

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Issa M El Nahhal ◽  
Shehata M Zourab ◽  
Fawzi S Kodeh ◽  
Alaa I Qudaih

2015 ◽  
Vol 1805 ◽  
Author(s):  
Jessica C. Fernandes ◽  
Marcelo Mulato

ABSTRACTZinc oxide thin films prepared by sol-gel and doped with different aluminum percentages were deposited by spray-pyrolysis deposition techniques on FTO and ITO substrates. The films were applied as the sensitive layer of pH-sensors EGFET devices. ZnO:Al films on FTO substrates shows a decrease in sensor quality with the dopant percentage increase. The opposite occurs when the substrate was ITO. The best quality film to be used as pH-sensor on FTO substrates was the ZnO:Al (3%) with 29 mV/pH sensitivity value and linear adjust of 0.99. For ITO substrates the best quality was achieved for ZnO:Al (10%), with sensitivity about 30 mV/pH and 0.99 linear adjust.


2021 ◽  
Author(s):  
Shazia Naheed ◽  
Shumaila Syed ◽  
Tahira Amir ◽  
Faisal Nawaz ◽  
Asia Bibi ◽  
...  

Abstract In this study a comprehensive investigation of doping of sol-gel silica with efficient metal chelating agents is provided at room temperature using infrared spectroscopy, elemental analysis, SEM, EDS and XRD. Sol-gel silica was doped with a series of metal chelating agents to investigate the sorption of Ni (II) ions from aqueous media. It is observed that the chelating agents are encapsulated by the pores of the xerogel from where they interact with the metal ions by ion exchange and chelation mechanism. Nickel complexes of the chelating agents were also synthesized. Sol-gel silica was doped with metal complexes for comparison with sol-gel silica having sorbed metal ions. Same amount of Ni (II) was observed in both samples, showing high efficiency of the method for removal of Ni (II). The batch adsorption experiments were performed at room temperature and neutral pH. The advantage of the method is high sorption capacity at room temperature and neutral pH. The developed method can be applied on large scale removal of toxic heavy metals. The sorption process completed within a minute. 0.1 N HNO3 resulted in complete desorption of metal ions from the gels. The regenerated sorbents were reused several times with negligible loss of sorption capacity.


Author(s):  
Hong Dinh Duong ◽  
Sung-Duk Oh ◽  
Jong Il Rhee

In this study, two heterogeneous fluorescence immunoassays using CdSe/ZnS quantum dot (QD) to label anti-progesterone antibody (P4Ab) for the determination of progesterone (P4) were performed in the wells of a 96-well microtiter plate. First, P4Ab was conjugated to hydrophilic CdSe/ZnS QDs via ethyl-3-(dimethylaminopropyl) carbodiimide(EDC)-N-hydroxysuccinimide) chemistry(NHS) (QDs-P4Ab conjugates). The QDs-P4Ab conjugate was employed as a second antibody in a sandwich assay, where the P4Ab was immobilized onto the 3-aminopropyltrimethoxysilane (APTMS) sol-gel membrane of the wells of a 96-well microtiter plate, and P4 was bound between the immobilized P4Ab and the QDs-P4Ab conjugate. In this assay, the fluorescence intensity of the QDs increased with increasing P4 concentrations. This assay had a detection limit of 553.9 pg/ml and a sensitivity of 18,251.96 pg/ml with a linear range of 2,184.6 – 117,082 pg/ml. In the direct binding assay, P4 was directly bound to the QDs-P4Ab conjugates immobilized onto the APTMS sol-gel membrane of the wells of a 96-well microtiter plate. In this direct binding assay the fluorescence intensity of the QDs decreased with increasing P4 concentrations, and this assay had a linear range of 28.95 – 26,607.7 pg/ml with a detection limit of 3.32 pg/ml and a sensitivity of 987.24 pg/ml. These fluorescence immunoassays have been successfully applied for the determination of P4 in real human serum, and the results were well correlated with those of a certified radioimmunoassay (RIA) method.


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