scholarly journals Rapid detection of hydrogen sulfide produced by pathogenic bacteria in focused growth media using SHS-MCC-GC-IMS

2018 ◽  
Vol 140 ◽  
pp. 232-240 ◽  
Author(s):  
Ryan Thompson ◽  
John D. Perry ◽  
Stephen P. Stanforth ◽  
John R. Dean
2014 ◽  
Vol 203 ◽  
pp. 596-601 ◽  
Author(s):  
Yutao Yang ◽  
Caixia Yin ◽  
Fangjun Huo ◽  
Yongbin Zhang ◽  
Jianbin Chao

Author(s):  
Gaowa Xing ◽  
Weifei Zhang ◽  
Nan Li ◽  
Qiaosheng Pu ◽  
Jin-Ming Lin

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.


2015 ◽  
Vol 87 (17) ◽  
pp. 8964-8969 ◽  
Author(s):  
Wei Zhang ◽  
Junqing Kang ◽  
Ping Li ◽  
Hui Wang ◽  
Bo Tang

2018 ◽  
Vol 16 (5) ◽  
pp. 712-716 ◽  
Author(s):  
Yandong Dou ◽  
Xiaoxu Gu ◽  
Shasha Ying ◽  
Shen Zhu ◽  
Shian Yu ◽  
...  

A fluorogenic probe based on the novel fluorophore 5-triazole-quinoline was developed for the detection of hydrogen sulfide, an endogenous signaling molecule associated with the development of various diseases.


2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Kamel S. Sultan ◽  
Tamer A. Ali ◽  
Nada A. Fahmy ◽  
Ayman El‐Shibiny

2017 ◽  
Vol 16 (6) ◽  
pp. 1188-1205 ◽  
Author(s):  
Diana C. Vanegas ◽  
Carmen L. Gomes ◽  
Nicholas D. Cavallaro ◽  
Daniel Giraldo-Escobar ◽  
Eric S. McLamore

2015 ◽  
Vol 39 (11) ◽  
pp. 8940-8947 ◽  
Author(s):  
Sima Paul ◽  
Shyamaprosad Goswami ◽  
Chitrangada Das Mukhopadhyay

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH− both in solid and vapour phases and this probe is used for fluorescence imaging of SH− in living cells.


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