Ultrasound-assisted purge-and-trap extraction for simultaneous determination of low-molecular weight amines and ammonium in high salinity waters by ion chromatography

2017 ◽  
Vol 133 ◽  
pp. 658-662 ◽  
Author(s):  
Fernanda Nunes Ferreira ◽  
Julio Carlos Afonso ◽  
Fernanda Veronesi Marinho Pontes ◽  
Manuel Castro Carneiro ◽  
Arnaldo Alcover Neto ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Simultaneous Determination ◽  
Ion Chromatography ◽  
High Salinity ◽  
Low Molecular Weight ◽  
Purge And Trap ◽  
Ultrasound Assisted

scholarly journals Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3321
Author(s):  
Katarzyna Kurpet ◽  
Rafał Głowacki ◽  
Grażyna Chwatko
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Reversed Phase ◽  
Fluorescence Labeling ◽  
Detector Response ◽  
Low Molecular Weight

Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

Purge-and-trap ion chromatography for the determination of trace ammonium ion in high-salinity water samples

2008 ◽  
Vol 1188 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Po-Yen Wang ◽  
Jing-Yi Wu ◽  
Hung-Jhen Chen ◽  
Tzung-Yi Lin ◽  
Chien-Hou Wu
Keyword(s):  
Ion Chromatography ◽  
Water Samples ◽  
High Salinity ◽  
Ammonium Ion ◽  
Purge And Trap ◽  
High Salinity Water ◽  
Salinity Water

Determination of water-soluble low-molecular-weight organic acids in soils by ion chromatography

2001 ◽  
Vol 47 (2) ◽  
pp. 387-397 ◽  
Author(s):  
Masayuki Tani ◽  
Kayoko Shinjo Shida ◽  
Kiyoshi Tsutsuki ◽  
Renzo Kondo
Keyword(s):  
Molecular Weight ◽  
Organic Acids ◽  
Ion Chromatography ◽  
Water Soluble ◽  
Low Molecular Weight

Determination of low-molecular-weight amines and ammonium in saline waters by ion chromatography after their extraction by steam distillation

2016 ◽  
Vol 39 (8) ◽  
pp. 1454-1460 ◽  
Author(s):  
Fernanda Nunes Ferreira ◽  
Julio Carlos Afonso ◽  
Fernanda Veronesi Marinho Pontes ◽  
Manuel Castro Carneiro ◽  
Arnaldo Alcover Neto ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Ion Chromatography ◽  
Steam Distillation ◽  
Low Molecular Weight ◽  
Saline Waters
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