Multiclass profiling of lipids of archaeological interest by ultra-high pressure liquid chromatography-atmospheric pressure chemical ionization-high resolution mass spectrometry

2017 ◽  
Vol 132 ◽  
pp. 49-58 ◽  
Author(s):  
Jose A. Tuñón-López ◽  
Miriam Beneito-Cambra ◽  
José Robles-Molina ◽  
David J. Parras-Guijarro ◽  
Antonio Molina-Díaz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
High Resolution Mass Spectrometry ◽  
Atmospheric Pressure Chemical Ionization ◽  
Ultra High Pressure ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical ◽  
Resolution Mass

scholarly journals Metabolite Profiling of Manilkara zapota L. Leaves by High-Resolution Mass Spectrometry Coupled with ESI and APCI and In Vitro Antioxidant Activity, α-Glucosidase, and Elastase Inhibition Assays

2020 ◽  
Vol 22 (1) ◽  
pp. 132
Author(s):  
Syful Islam ◽  
Md Badrul Alam ◽  
Hyeon-Jin Ann ◽  
Ji-Hyun Park ◽  
Sang-Han Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
High Resolution Mass Spectrometry ◽  
Atmospheric Pressure Chemical Ionization ◽  
Ionization Mass ◽  
Manilkara Zapota ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

High-resolution mass spectrometry equipped with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources was used to enhance the characterization of phytochemicals of ethanol extracts of Manilkara zapota L. leaves (ZLE). Sugar compounds, dicarboxylic acids, compounds of phenolic acids and flavonoids groups, and other phytochemicals were detected from the leaves. Antioxidant activity and inhibition potentiality of ZLE against α-glucosidase enzyme, and elastase enzyme activities were evaluated in in vitro analysis. ZLE significantly inhibited activities of α-glucosidase enzyme at a lower concentration (IC50 2.51 ± 0.15 µg/mL). Glucose uptake in C2C12 cells was significantly enhanced by 42.13 ± 0.15% following the treatment with ZLE at 30 µg/mL. It also exhibited potential antioxidant activities and elastase enzyme inhibition activity (IC50 27.51 ± 1.70 µg/mL). Atmospheric pressure chemical ionization mass spectrometry (APCI–MS) detected more m/z peaks than electrospray ionization mass spectrometry (ESI–MS), and both ionization techniques illustrated the biological activities of the detected compounds more thoroughly compared to single-mode analysis. Our findings suggest that APCI along with ESI is a potential ionization technique for metabolite profiling, and ZLE has the potential in managing diabetes by inhibiting α-glucosidase activity and enhancing glucose uptake.

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