Electrochemical sensor for the determination of enterotoxigenic Escherichia coli in swine feces using glassy carbon electrodes modified with multi-walled carbon nanotubes

2016 ◽  
Vol 127 ◽  
pp. 220-225 ◽  
Author(s):  
Lorena Viviana Tarditto ◽  
Fernando Javier Arévalo ◽  
María Alicia Zon ◽  
Hugo García Ovando ◽  
Nelio Roberto Vettorazzi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Nanotubes ◽  
Glassy Carbon ◽  
Enterotoxigenic Escherichia Coli ◽  
Carbon Electrodes ◽  
Glassy Carbon Electrodes ◽  
Multi Walled Carbon Nanotubes ◽  
Swine Feces ◽  
Walled Carbon Nanotubes

scholarly journals Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrodes modified by multi-walled carbon nanotubes

2010 ◽  
Vol 1 ◽  
pp. 135-141 ◽  
Author(s):  
Haitao Zheng ◽  
Leyi Lin ◽  
Yosuke Okezaki ◽  
Ryushi Kawakami ◽  
Haruhiko Sakuraba ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Active Site ◽  
Physical Adsorption ◽  
Carbon Electrodes ◽  
Proline Dehydrogenase ◽  
Glassy Carbon Electrodes ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

A glassy carbon electrode (GC) was modified by multi-walled carbon nanotubes (MWCNTs). The modified electrode showed a pair of redox peaks that resulted from the oxygen-containing functional groups on the nanotube surface. A recombinant thermostable dye-linked L-proline dehydrogenase (L-proDH) from hyperthermophilic archaeon (Thermococcus profundus) was further immobilized by physical adsorption. The modified electrode (GC/MWCNTs/L-proDH) exhibited an electrocatalytic signal for L-proline compared to bare GC, GC/L-proDH and GC/MWCNTs electrodes, which suggested that the presence of MWCNTs efficiently enhances electron transfer between the active site of enzyme and electrode surface. The immobilized L-proDH showed a typical Michaelis–Menten catalytic response with lower apparent constant.

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