scholarly journals Simultaneous analysis of 25 phenolic compounds in grape juice for HPLC: Method validation and characterization of São Francisco Valley samples

2013 ◽  
Vol 110 ◽  
pp. 665-674 ◽  
Author(s):  
Mariana Mirelle Pereira Natividade ◽  
Luiz Claudio Corrêa ◽  
Scheilla Vitorino Carvalho de Souza ◽  
Giuliano Elias Pereira ◽  
Luiz Carlos de Oliveira Lima
Keyword(s):  
Phenolic Compounds ◽  
Method Validation ◽  
Grape Juice ◽  
Hplc Method ◽  
Simultaneous Analysis

Fast quantitative determination of phenolic compounds in grape juice by UPLC-MS: method validation and characterization of juices produced with different grape varieties

2020 ◽  
Author(s):  
Natalia Alejandra Pisoni Canedo-Reis ◽  
Celito Crivellaro Guerra ◽  
Letícia Flores da Silva ◽  
Luísa Carolina Wetzstein ◽  
Carlos Henrique Junges ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Method Validation ◽  
Grape Juice ◽  
Grape Varieties

scholarly journals Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil

2018 ◽  
Vol 66 ◽  
pp. 160-167 ◽  
Author(s):  
Emanuela Monteiro Coelho ◽  
Carla Valéria da Silva Padilha ◽  
Gabriela Aquino Miskinis ◽  
Antônio Gomes Barroso de Sá ◽  
Giuliano Elias Pereira ◽  
...  
Keyword(s):  
Organic Acids ◽  
Method Validation ◽  
Hplc Method ◽  
Simultaneous Analysis ◽  
Northeast Brazil ◽  
Grape Juices

scholarly journals Analysis of Homoisoflavonoids in Caesalpinia digyna by HPLC-ESI-MS, HPLC and Method Validation

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Somendu K. Roy ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Method Validation ◽  
Mobile Phase ◽  
Hplc Method ◽  
Good Recovery ◽  
Esi Ms ◽  
Validation Parameters ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc ◽  
Hplc Analyses

The roots of Caesalpinia digyna have been reported to contain gallic acid derivatives and minor homoisoflavonoids, but HPLC-ESI-MS and HPLC analyses of the homoisoflavonoids were challenging due to their low concentration in the roots. Separation and identification was accomplished by HPLC-ESI-MS and further elaborated for quantification using a C18 column with detection at 330 nm. A gradient mobile phase consisting of methanol and water (0.1% acetic acid) was used. The developed HPLC method showed good linearity (r2≥0.998), high precision (RSD<5%) and a good recovery (99.3-104.5%) of the compounds. The lowest detection limit was 0.75 ng and the method was found to be robust. All the validation parameters were found to be within the permissible limits and, therefore, the developed method is accurate and reliable for the quality control of C. digyna and other Caesalpinia species. This is the first report of sample preparation on Diaion HP-20 resin and characterization of homoisoflavonoids by HPLC-ESI-MS, extended by extensive quantitative HPLC analysis of homoisoflavonoids in C. digyna roots and method validation.

scholarly journals Determining 1-kestose, nystose and raffinose oligosaccharides in grape juices and wines using HPLC: method validation and characterization of products from Northeast Brazil

2019 ◽  
Vol 56 (10) ◽  
pp. 4575-4584 ◽  
Author(s):  
Marcos dos Santos Lima ◽  
Polyana Campos Nunes ◽  
Bernadete de Lourdes de Araújo Silva ◽  
Carla Valéria da Silva Padilha ◽  
Thaís Helena Figueiredo do Bonfim ◽  
...  
Keyword(s):  
Method Validation ◽  
Hplc Method ◽  
Northeast Brazil ◽  
Raffinose Oligosaccharides ◽  
Grape Juices

Synthesis and Characterization of Potential and Degraded Impurities of Regadenoson

2020 ◽  
Vol 16 (8) ◽  
pp. 1130-1139
Author(s):  
Singaram Sathiyanarayanan ◽  
Chidambaram Subramanian Venkatesan ◽  
Senthamaraikannan Kabilan
Keyword(s):  
Mass Spectra ◽  
Degradation Products ◽  
Hplc Method ◽  
Forced Degradation ◽  
Spectroscopic Techniques ◽  
A2a Adenosine Receptor ◽  
Degradation Study ◽  
Related Substances ◽  
Adenosine Receptor Agonist

Background: Regadenoson is an A2A adenosine receptor agonist that is a coronary vasodilator and commonly used as a pharmacologic cardiac stressing agents. Methods: HPLC method was used for the analysis of related substances. The degraded impurities during the process were isolated and characterized by IR, Mass and NMR spectral analysis. Results: Forced degradation study of regadenoson under conditions of hydrolysis (neutral, acidic and alkaline) and oxidations suggested in the ICH Q1A(R2) was accomplished. The drug showed significant degradation under all the above conditions. On the whole, five novel degradation products were found under diverse conditions along with process related impurities which were not reported earlier. Conclusion: All the degradation products were well characterized by using advanced spectroscopic techniques like IR, 1H NMR, 13C NMR and Mass spectra. The identification of these impurities will be productive for the quality control during the production and stability behavior of the regadenoson drug substance.

scholarly journals Processing Effect and Characterization of Olive Oils from Spanish Wild Olive Trees (Olea europaea var. sylvestris)

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1304
Author(s):  
Francisco Espínola ◽  
Alfonso M. Vidal ◽  
Juan M. Espínola ◽  
Manuel Moya
Keyword(s):  
Phenolic Compounds ◽  
Volatile Compounds ◽  
Olive Tree ◽  
Extraction Yield ◽  
Point Of View ◽  
Human Consumption ◽  
Analytical Point ◽  
Wild Olive ◽  
Olive Trees

Wild olive trees have important potential, but, to date, the oil from wild olives has not been studied significantly, especially from an analytical point of view. In Spain, the wild olive tree is called “Acebuche” and its fruit “Acebuchina”. The objective of this work is to optimize the olive oil production process from the Acebuchina cultivar and characterize the oil, which could be marketed as healthy and functional food. A Box–Behnken experimental design with five central points was used, along with the Response Surface Methodology to obtain a mathematical experimental model. The oils from the Acebuchina cultivar meet the requirements for human consumption and have a good balance of fatty acids. In addition, the oils are rich in antioxidants and volatile compounds. The highest extraction yield, 12.0 g oil/100 g paste, was obtained at 90.0 min and the highest yield of phenolic compounds, 870.0 mg/kg, was achieved at 40.0 °C, and 90.0 min; but the maximum content of volatile compounds, 26.9 mg/kg, was obtained at 20 °C and 30.0 min. The oil yield is lower than that of commercial cultivars, but the contents of volatile and phenolic compounds is higher.

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