scholarly journals Fourier transform infrared microspectroscopy as a bacterial source tracking tool to discriminate fecal E. coli strains

2011 ◽  
Vol 99 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Camila Carlos ◽  
Danilo A. Maretto ◽  
Ronei J. Poppi ◽  
Maria Inês Z. Sato ◽  
Laura M.M. Ottoboni
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Source Tracking ◽  
E Coli ◽  
Bacterial Source Tracking ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy

scholarly journals Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
V. Erukhimovitch ◽  
M. Huleihil ◽  
M. Huleihel
Keyword(s):  
Cell Culture ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Vero Cells ◽  
Ftir Microscopy ◽  
E Coli ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Simplex Virus

Fourier transform infrared microspectroscopy (FTIR-M) can detect small molecular changes in cells and therefore was previously applied for the identification of different biological samples. In the present study, FTIR spectroscopy was used for the identification and discrimination of Vero cells infected with herpes viruses or contaminated with bacteria or fungi in cell culture. Vero cells in culture were infected herpes simplex virus type 1 (HSV-1) or contaminated withE. colibacteria orCandida albicansfungi and analyzed by FTIR microscopy at 24 h postinfection/contamination. Specific different spectral changes were observed according to the infecting or contaminating agent. For instance, both pure fungi and cell culture contaminated with this fungi showed specific peaks at 1030 cm−1and at 1373 cm−1regions, while pureE. coliand cell culture contaminated with this bacteria showed a specific and unique peak at 1657 cm−1. These results support the potential of developing FTIR microspectroscopy as a simple, reagent free method for identification and discrimination between different tissue infection or contamination with various pathogens.

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