Possibilities and limitations of the sequential injection chromatography technique for the determination of anticoccidial agents in water, pharmaceutical formulations and feed

2011 ◽  
Vol 98 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Erland Björklund ◽  
Fernando Maya ◽  
Søren A. Bak ◽  
Martin Hansen ◽  
José Manuel Estela ◽  
...  
Keyword(s):  
Pharmaceutical Formulations ◽  
Sequential Injection ◽  
Sequential Injection Chromatography

Sequential Injection Chromatography with Monolithic Column for Phenothiazines Assay in Human Urine and Pharmaceutical Formulations

2020 ◽  
Vol 16 (7) ◽  
pp. 967-975
Author(s):  
Abubakr M. Idris
Keyword(s):  
Phase Composition ◽  
Human Urine ◽  
Mobile Phase ◽  
Monolithic Column ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Formulation ◽  
Assay Method ◽  
Sequential Injection ◽  
Mobile Phase Composition ◽  
Sequential Injection Chromatography

Methods: Sequential injection chromatography (SIC) with monolithic column has been proposed with potential benefits for separation and quantification. Objective: To utilize SIC to develop a new assay method for the separation and quantification of some phenothiazines (promethazine, chlorpromazine and perphenazine) in human urine and synthetic pharmaceutical formulations. Methods: The 32 full-factorial design was adopted to study the effect of mobile phase composition on separation efficiency, retention time, peak height and baseline. The separation was conducted on a C18 monolithic column (100 × 4.6 mm) using a mobile phase composition of phosphate: acetonitrile:methanol (60:28:12) at pH 4.0. The detection was carried out using a miniaturized fiber optic spectrometer at 250 nm. Results: Satisfactory analytical features, including number of theoretical plates (1809-6232), peak symmetry (1.0-1.3), recovery (95.5-99.1% in pharmaceutical formulations and 91.6-94.7% in urine), intra-day precision (0.36-1.60% for pharmaceutical formulation and 2.96-3.67 for urine), inter-day precision (1.47-2.28% for pharmaceutical formulation), limits of detection (0.23-0.88 μg/ml) and limits of quantification (0.77-2.90 μg/ml), were obtained. Conclusion: The remarkable advantages of the proposed SIC method are the inexpensiveness in terms of instrumentation and reagent consumption.

Determination of pesticides fenoxycarb and permethrin by sequential injection chromatography using miniaturized monolithic column

Talanta ◽  
2008 ◽  
Vol 77 (2) ◽  
pp. 566-570 ◽  
Author(s):  
P CHOCHOLOUS ◽  
D SATINSKY ◽  
R SLADKOVSKY ◽  
M POSPISILOVA ◽  
P SOLICH
Keyword(s):  
Monolithic Column ◽  
Sequential Injection ◽  
Sequential Injection Chromatography

Advantages of core–shell particle columns in Sequential Injection Chromatography for determination of phenolic acids

Talanta ◽  
2013 ◽  
Vol 103 ◽  
pp. 221-227 ◽  
Author(s):  
Petr Chocholouš ◽  
Jana Vacková ◽  
Ivana Šrámková ◽  
Dalibor Šatínský ◽  
Petr Solich
Keyword(s):  
Phenolic Acids ◽  
Core Shell ◽  
Sequential Injection ◽  
Shell Particle ◽  
Sequential Injection Chromatography

On-column preconcentration in sequential injection chromatography: application to determination of parabens

2015 ◽  
Vol 7 (10) ◽  
pp. 4371-4375 ◽  
Author(s):  
Alex D. Batista ◽  
Fábio R. P. Rocha
Keyword(s):  
Enrichment Factors ◽  
Sequential Injection ◽  
Sequential Injection Chromatography ◽  
High Enrichment ◽  
Column Preconcentration

High enrichment factors were achieved by retaining the analytes at the head of the column in sequential injection chromatography, without hindering resolution or retention times.

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