scholarly journals Long-term incomplete xylose fermentation, after glucose exhaustion, with Candida shehatae co-immobilized with Saccharomyces cerevisiae

2007 ◽  
Vol 162 (3) ◽  
pp. 211-218 ◽  
Author(s):  
T. Lebeau ◽  
T. Jouenne ◽  
G.-A. Junter
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Fermentation ◽  
Candida Shehatae

scholarly journals Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae

2021 ◽  
Vol 9 ◽  
Author(s):  
Minhye Shin ◽  
Heeyoung Park ◽  
Sooah Kim ◽  
Eun Joong Oh ◽  
Deokyeol Jeong ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Fermentation ◽  
Sugar Metabolism ◽  
Pentose Phosphate ◽  
Biofuel Production ◽  
Rna Seq ◽  
Loss Of Function ◽  
Lignocellulosic Biofuel ◽  
Pentose Sugar ◽  
Transcriptomic Changes

Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has been reported to be a crucial genetic perturbation in improving xylose fermentation. A confirmed mechanism of the Δpho13 effect on xylose fermentation is that the Δpho13 transcriptionally activates the genes in the non-oxidative pentose phosphate pathway (PPP). In the current study, we found a couple of engineered strains, of which phenotypes were not affected by Δpho13 (Δpho13-negative), among many others we examined. Genome resequencing of the Δpho13-negative strains revealed that a loss-of-function mutation in GCR2 was responsible for the phenotype. Gcr2 is a global transcriptional factor involved in glucose metabolism. The results of RNA-seq confirmed that the deletion of GCR2 (Δgcr2) led to the upregulation of PPP genes as well as downregulation of glycolytic genes, and changes were more significant under xylose conditions than those under glucose conditions. Although there was no synergistic effect between Δpho13 and Δgcr2 in improving xylose fermentation, these results suggested that GCR2 is a novel knockout target in improving lignocellulosic ethanol production.

scholarly journals Induction of the synthesis of an additional family of long-chain dolichols in the yeast Saccharomyces cerevisiae. Effect of starvation and ageing.

2002 ◽  
Vol 49 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Anna Szkopinska ◽  
Ewa Swiezewska ◽  
Joanna Rytka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stationary Phase ◽  
Yeast Cells ◽  
Logarithmic Phase ◽  
Cellular Membranes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chemical Changes ◽  
Physico Chemical ◽  
Long Chain

The yeast Saccharomyces cerevisiae strain W303 synthesizes in the early logarithmic phase of growth dolichols of 14-18 isoprene residues. The analysis of the polyisoprenoids present in the stationary phase revealed an additional family which proved to be also dolichols but of 19-24 isoprene residues, constituting 39% of the total dolichols. The transfer of early logarithmic phase cells to a starvation medium lacking glucose or nitrogen resulted in the synthesis of the longer chain dolichols. The additional family of dolichols represented 13.8% and 10.3% of total dolichols in the glucose and nitrogen deficient media, respectively. The level of dolichols in yeast cells increased with the age of the cultures. Since both families of dolichols are present in stationary phase cells we postulate that the longer chain dolichols may be responsible for the physico-chemical changes in cellular membranes allowing yeast cells to adapt to nutrient deficient conditions to maintain long-term viability.

scholarly journals Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects

2016 ◽  
Vol 17 (3) ◽  
pp. 207 ◽  
Author(s):  
Danuza Moysés ◽  
Viviane Reis ◽  
João Almeida ◽  
Lidia Moraes ◽  
Fernando Torres
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Fermentation

Long-term effects of restrictive culture conditions on Saccharomyces cerevisiae sec7 cells

1996 ◽  
Vol 151 (1) ◽  
pp. 93-97
Author(s):  
Christiane Kühnel ◽  
Regina Stoltenburg ◽  
Irene Kunze ◽  
Gotthard Kunze
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Culture Conditions ◽  
Long Term Effects

gTME for Improved Xylose Fermentation of Saccharomyces cerevisiae

2008 ◽  
Vol 160 (2) ◽  
pp. 574-582 ◽  
Author(s):  
Hongmei Liu ◽  
Ming Yan ◽  
Cangang Lai ◽  
Lin Xu ◽  
Pingkai Ouyang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Fermentation
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