Proteomic analysis on the regulation of DOPA-melanin synthesis in Talaromyces marneffei

2021 ◽  
Vol 150 ◽  
pp. 104701
Author(s):  
Xiaoyue He ◽  
Donghua Liu ◽  
Qicong Chen
Keyword(s):  
Proteomic Analysis ◽  
Melanin Synthesis ◽  
Talaromyces Marneffei ◽  
Dopa Melanin

Characterization and role of tyrosinases in the lichenLobaria pulmonaria(L.) Hoffm.

2016 ◽  
Vol 48 (4) ◽  
pp. 311-322 ◽  
Author(s):  
Lusanda P. MATEE ◽  
Richard P. BECKETT ◽  
Knut A. SOLHAUG ◽  
Farida V. MINIBAYEVA
Keyword(s):  
Enzyme Activity ◽  
Tyrosinase Activity ◽  
Melanin Synthesis ◽  
Direct Sunlight ◽  
Free Living ◽  
Lichenized Ascomycetes ◽  
Lobaria Pulmonaria ◽  
Dopa Melanin

AbstractTyrosinases are a widespread family of multicopper oxidase enzymes. In our earlier work, we identified the presence of tyrosinases in lichenized Ascomycetes based on their substratum specificity, sensitivity to inhibitors and molecular mass. Here, we present a more detailed characterization of a tyrosinase from the lichenLobaria pulmonaria. We also compare tyrosinase activity with the activities of laccases and peroxidases, the other redox enzymes present in this species. The importance of tyrosinases in lichen biology was studied by testing their role in melanin synthesis. Laboratory experiments clearly showed that tyrosinases fromL. pulmonariaresemble those from other lichens and in free-living fungi. While the tyrosinases can metabolize the melanin precursor L-DOPA, L-DOPA can also be metabolized by peroxidases and laccases. A field experiment showed that exposing shade-adaptedL. pulmonariato normal solar radiation induces L-DOPA melanin synthesis. Synthesis occurred when lichens were exposed to either direct sunlight, or placed under a wavelength-neutral filter that slightly reduced overall light. In lichens receiving unfiltered sunlight, melanin synthesis was accompanied by increased laccase activity; by contrast, no changes in enzyme activity occurred in lichens placed under the wavelength-neutral filter. Melanization was reduced by placing lichens under filters that removed UV-B, and prevented by filters that removed both UV-A and UV-B. Removing UV-B had no effect on enzyme activity, whereas removing both UV-A and UV-B increased tyrosinase activity. Results from this study indicate that under some conditions laccases may be involved in melanin synthesis, but they provide no evidence for a role for tyrosinases in melanization. Although high tyrosinase activities are widespread in lichens, many questions on the role of this enzyme in lichen biology remain to be answered.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Proteomic analysis of the anti-obesity effect of Taeumjowui-Tang in high fat diet-induced obese mice

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Changkyun Park ◽  
SY Lee ◽  
SH Yun ◽  
WY Kim ◽  
Y Yi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat

Proteomic analysis of human testicular interstitial fluid reflects disordered spermatogenesis in Klinefelter's syndrome

2014 ◽  
Author(s):  
Robert I McLachlan ◽  
Andrew N Stephens ◽  
Adam Rainczuk ◽  
Caroline Foo ◽  
Mark R Condina ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Interstitial Fluid ◽  
Klinefelter’S Syndrome ◽  
Klinefelter's Syndrome

Proteomic analysis of nuclear receptor coregulator complexes: II

2013 ◽  
Author(s):  
SY Jung ◽  
A Malovannaya ◽  
J Wei ◽  
BW O'Malley ◽  
J Qin
Keyword(s):  
Nuclear Receptor ◽  
Proteomic Analysis
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