scholarly journals Identification B and T-Cell epitopes and functional exposed amino acids of S protein as a potential vaccine candidate against SARS-CoV-2/COVID-19

2020 ◽  
Vol 148 ◽  
pp. 104459
Author(s):  
Maryam Tohidinia ◽  
Fatemeh Sefid
Keyword(s):  
Amino Acids ◽  
T Cell ◽  
Vaccine Candidate ◽  
T Cell Epitopes ◽  
S Protein ◽  
Potential Vaccine Candidate ◽  
Potential Vaccine

Improvement of the attenuated mutant strain of Brucella melitensis Rev1 as a potential vaccine candidate

Biologia ◽  
2021 ◽  
Author(s):  
Elham Mehdizadeh Marzenaki ◽  
Ali Reza Saeedinia ◽  
Mehdi Zeinoddini ◽  
Ali Asghar Deldar
Keyword(s):  
Mutant Strain ◽  
Vaccine Candidate ◽  
Brucella Melitensis ◽  
Potential Vaccine Candidate ◽  
Potential Vaccine

scholarly journals Moraxella catarrhalis Bacterium without Endotoxin, a Potential Vaccine Candidate

2005 ◽  
Vol 73 (11) ◽  
pp. 7569-7577 ◽  
Author(s):  
Daxin Peng ◽  
Wenzhou Hong ◽  
Biswa P. Choudhury ◽  
Russell W. Carlson ◽  
Xin-Xing Gu
Keyword(s):  
Moraxella Catarrhalis ◽  
Lipid A ◽  
Vaccine Candidate ◽  
Gene Encoding ◽  
Potential Vaccine Candidate ◽  
Whole Cells ◽  
Potential Vaccine ◽  
Major Surface ◽  
Membrane Components ◽  
Human Infections

ABSTRACT Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of the bacterium. An lpxA gene encoding UDP-N-acetylglucosamine acyltransferase responsible for the first step of lipid A biosynthesis was identified by the construction and characterization of an isogenic M. catarrhalis lpxA mutant in strain O35E. The resulting mutant was viable despite the complete loss of LOS. The mutant strain showed significantly decreased toxicity by the Limulus amebocyte lysate assay, reduced resistance to normal human serum, reduced adherence to human epithelial cells, and enhanced clearance in lungs and nasopharynx in a mouse aerosol challenge model. Importantly, the mutant elicited high levels of antibodies with bactericidal activity and provided protection against a challenge with the wild-type strain. These data suggest that the null LOS mutant is attenuated and may be a potential vaccine candidate against M. catarrhalis.

scholarly journals Towards large-scale identification of HLA-restricted T cell epitopes from four vaccine candidate antigens in a malaria endemic community in Ghana

Vaccine ◽  
2021 ◽  
Author(s):  
Kwadwo Asamoah Kusi ◽  
Ebenezer Addo Ofori ◽  
Kwadwo Akyea-Mensah ◽  
Eric Kyei-Baafour ◽  
Augustina Frimpong ◽  
...  
Keyword(s):  
T Cell ◽  
Large Scale ◽  
Vaccine Candidate ◽  
T Cell Epitopes

scholarly journals Large Extracellular Loop of Tetraspanin as a Potential Vaccine Candidate for Filariasis

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77394 ◽  
Author(s):  
Gajalakshmi Dakshinamoorthy ◽  
Gnanasekar Munirathinam ◽  
Kristen Stoicescu ◽  
Maryada Venkatarami Reddy ◽  
Ramaswamy Kalyanasundaram
Keyword(s):  
Vaccine Candidate ◽  
Extracellular Loop ◽  
Potential Vaccine Candidate ◽  
Large Extracellular Loop ◽  
Potential Vaccine

scholarly journals T-Cell Epitopes in Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Protein Elicit a Specific T-Cell Immune Response in Patients Who Recover from SARS

2004 ◽  
Vol 78 (11) ◽  
pp. 5612-5618 ◽  
Author(s):  
Yue-Dan Wang ◽  
Wan-Yee Fion Sin ◽  
Guo-Bing Xu ◽  
Huang-Hua Yang ◽  
Tin-yau Wong ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Epitope ◽  
T Cell Response ◽  
Cell Immune Response ◽  
T Cell Epitopes ◽  
Online Database ◽  
Database Analysis ◽  
S Protein ◽  
Healthy Donors ◽  
Ifn Γ

ABSTRACT The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-γ) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-γ-secreting T-cell response in HLA-A2+ donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2+ healthy donors or in HLA-A2− donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2+ SARS-CoV-infected patients.

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