Computational probing of Toxoplasma gondii major surface antigen 1 (SAG1) for enhanced vaccine design against toxoplasmosis

2020 ◽  
Vol 147 ◽  
pp. 104386 ◽  
Author(s):  
Hamidreza Majidiani ◽  
Abdolhossein Dalimi ◽  
Fatemeh Ghaffarifar ◽  
Majid Pirestani ◽  
Ali Dalir Ghaffari
Keyword(s):  
Toxoplasma Gondii ◽  
Surface Antigen ◽  
Vaccine Design ◽  
Major Surface

scholarly journals Immunoinformatic prediction about potential novel vaccine in surface antigen fragment protein of Toxoplasma gondii

2016 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Seyed Sajjad Hasheminasab ◽  
Hossein Maghsood ◽  
Sara Khalili
Keyword(s):  
Toxoplasma Gondii ◽  
B Cell ◽  
Surface Antigen ◽  
Cell Epitope ◽  
Epitope Prediction ◽  
Font Size ◽  
B Cell Epitope ◽  
Major Surface ◽  
Antigen Fragment

<p class="MsoNormal" style="text-align: justify; text-justify: inter-ideograph;"><span style="font-size: 9.0pt;">Toxoplasmosis is one of the most widespread infections in animals and humans. The <em>Toxoplasma gondii</em> major surface antigen, called SAG1 or p30, is a highly immunogenic protein which has generated great interest as a diagnostic reagent, as a potential subunit vaccine, and for its role in invasion. In this study, the epitopes of <em>Toxoplasma gondii</em> SAG1 were identified using bioinformatics. Through the analysis of the out¬put of both NetCTL and CTLPred, and B-cell epitope prediction, the position of all the epitopes were found and combined in four sequences. The different tasks including, T-cell and B-cell prediction, Antigenicity determination of the conserved peptides, Homology modeling, Allergenicity and epitope conservancy analysis were done on the conserved peptides. We predict that our proposed epitopes would also trigger an immune response in vitro.</span></p><span style="font-size: 18.0pt; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Batang; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Immunoinformatic prediction about potential novel vaccine in surface antigen fragment protein of <em>Toxoplasma gondii</em></span>

scholarly journals Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding

Parasitology ◽  
2004 ◽  
Vol 128 (4) ◽  
pp. 391-396 ◽  
Author(s):  
S. A. ROBINSON ◽  
J. E. SMITH ◽  
P. A. MILLNER
Keyword(s):  
Toxoplasma Gondii ◽  
Host Cell ◽  
Surface Antigen ◽  
Cell Binding ◽  
Major Surface ◽  
Vitro Analysis ◽  
In Vitro Analysis

Epitopic analysis of the Toxoplasma gondii major surface antigen SAG1

1994 ◽  
Vol 66 (1) ◽  
pp. 31-38 ◽  
Author(s):  
F. Velge-Roussel ◽  
T. Chardès ◽  
P. Mévélec ◽  
M. Brillard ◽  
J. Hoebeke ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Surface Antigen ◽  
Major Surface

Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens

1989 ◽  
Vol 9 (10) ◽  
pp. 4576-4580
Author(s):  
S Tomavo ◽  
R T Schwarz ◽  
J F Dubremetz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Toxoplasma Gondii ◽  
Phospholipase C ◽  
Surface Antigens ◽  
Water Soluble ◽  
Radioactive Label ◽  
Glycosyl Phosphatidylinositol ◽  
Major Surface

The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.

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